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The map label for this gene is sufB [H]

Identifier: 225861236

GI number: 225861236

Start: 1271966

End: 1273378

Strand: Reverse

Name: sufB [H]

Synonym: SPT_1330

Alternate gene names: 225861236

Gene position: 1273378-1271966 (Counterclockwise)

Preceding gene: 225861237

Following gene: 225861234

Centisome position: 60.29

GC content: 41.83

Gene sequence:

>1413_bases
ATGGCTGAAGAAAGAGTAGAACCAAAACCAATTGACCTTGGTGAATATAAATTTGGTTTCCATGACGATGTAGAGCCTGT
CTTATCGACAGGAAAAGGACTCAACGAAGGTGTTATTCGTGAATTATCTGCTGTTAAGGGTGAGCCTGAGTGGATGTTGG
AGTTCCGTTTGAAGTCTTATGAAACCTTCAAAAAAATGCCCATGCAAACTTGGGGAGCAGACTTGTCAGAGATTGACTTT
GATGACTTAATCTACTACCAAAAACCATCTGACAAACCAGCCCGTTCTTGGGATGATGTACCTGAAAAGATTAAAGAAAC
CTTTGAACGTATCGGGATTCCAGAAGCTGAACGTGCTTATTTAGCAGGGGCTTCTGCCCAGTACGAGTCAGAAGTGGTTT
ACCACAACATGAAGGAAGAGTTCCAAAAATTAGGTATTATCTTTACAGATACAGATTCCGCACTCAAGGAATACCCAGAC
TTATTTAAACAATACTTTGCGAAGTTGGTACCGCCGACAGATAACAAGTTGGCAGCCCTCAACTCAGCAGTATGGTCGGG
TGGAACTTTTATCTACGTGCCAAAAGGTGTCAAGGTAGATATTCCACTTCAAACTTATTTCCGTATCAATAACGAAAATA
TAGGTCAGTTCGAACGTACCTTGATTATCGTTGATGAGGGAGCAAGCGTCCACTACGTAGAAGGATGTACAGCACCAACA
TATTCAAGCAATAGCTTGCACGCTGCCATTGTAGAAATTTTTGCTTTGGACGGAGCTTATATGCGTTATACAACTATCCA
AAACTGGTCTGATAACGTCTATAACTTGGTAACAAAGCGTGCTAAGGCTCAAAAGGATGCCACTGTTGAGTGGATTGATG
GAAACTTGGGTGCCAAAACGACTATGAAATATCCATCTGTTTACCTTGATGGAGAAGGAGCGCGTGGTACCATGCTCTCT
ATCGCCTTTGCTAATGCAGGGCAACACCAAGACACGGGTGCTAAGATGATTCACAATGCTCCACATACCAGCTCGTCTAT
TGTGTCTAAATCCATCGCTAAAGGTGGAGGAAAGGTTGACTACCGTGGACAAGTCACCTTTAACAAGAACTCTAAGAAAT
CTGTTTCCCACATTGAATGTGATACCATTATCATGGATGACTTGTCAGCATCAGATACTATTCCATTTAATGAAATTCAC
AACTCGCAAGTGGCTTTGGAACACGAAGCCAAAGTATCTAAGATTTCAGAAGAGCAATTGTATTATCTCATGAGCCGTGG
ATTGTCAGAATCTGAGGCAACTGAAATGATTGTCATGGGATTTGTAGAACCCTTTACAAAAGAACTTCCAATGGAATACG
CAGTTGAGCTGAACCGCTTGATTAGCTATGAAATGGAGGGATCAGTTGGATAA

Upstream 100 bases:

>100_bases
CTTGGAATGCCCTTAAGAAAACAATTGAAAATCAAGAAAAACAGTAAGACAAGTTTCTTTTGTCTTATGAATTATTAGAA
ATGAAGAAAGAAAGGATACT

Downstream 100 bases:

>100_bases
AATTTGATTTTATACTCTTCGAAAATCTCTTCAAACCACGTCAGCATCGCCTTACCGTATGTATGGTTACTGACTTCGTC
AGTTTCATCTACAACCTCAA

Product: FeS assembly protein SufB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSAVKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDF
DDLIYYQKPSDKPARSWDDVPEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFERTLIIVDEGASVHYVEGCTAPT
YSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNLVTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLS
IAFANAGQHQDTGAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASDTIPFNEIH
NSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELPMEYAVELNRLISYEMEGSVG

Sequences:

>Translated_470_residues
MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSAVKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDF
DDLIYYQKPSDKPARSWDDVPEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFERTLIIVDEGASVHYVEGCTAPT
YSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNLVTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLS
IAFANAGQHQDTGAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASDTIPFNEIH
NSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELPMEYAVELNRLISYEMEGSVG
>Mature_469_residues
AEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSAVKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFD
DLIYYQKPSDKPARSWDDVPEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPDL
FKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFERTLIIVDEGASVHYVEGCTAPTY
SSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNLVTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSI
AFANAGQHQDTGAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASDTIPFNEIHN
SQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELPMEYAVELNRLISYEMEGSVG

Specific function: The sufBCD complex acts synergistically with sufE to stimulate the cysteine desulfurase activity of sufS. The sufBCD complex contributes to the assembly or repair of oxygen-labile iron-sulfur clusters under oxidative stress. May facilitate iron uptake fro

COG id: COG0719

COG function: function code O; ABC-type transport system involved in Fe-S cluster assembly, permease component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0051 (ycf24) family [H]

Homologues:

Organism=Escherichia coli, GI87081955, Length=480, Percent_Identity=39.1666666666667, Blast_Score=344, Evalue=6e-96,
Organism=Escherichia coli, GI1787971, Length=154, Percent_Identity=27.9220779220779, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010231
- InterPro:   IPR000825 [H]

Pfam domain/function: PF01458 UPF0051 [H]

EC number: NA

Molecular weight: Translated: 52705; Mature: 52574

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSAVKGEPEWMLEFRLKSY
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHH
ETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPEKIKETFERIGIPEAERAY
HHHHHCCHHHHCCCHHHCCHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
LAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPDLFKQYFAKLVPPTDNKLAAL
HCCCCCCCCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
NSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFERTLIIVDEGASVHYVEGCTAPT
HHHHCCCCEEEEECCCCEEEECHHEEEEECCCCCCCEEEEEEEEECCCCEEEEECCCCCC
YSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNLVTKRAKAQKDATVEWIDGNLGAKT
CCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCE
TMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGAKMIHNAPHTSSSIVSKSIAKGGGKVD
EEECCEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCCEE
YRGQVTFNKNSKKSVSHIECDTIIMDDLSASDTIPFNEIHNSQVALEHEAKVSKISEEQL
EEEEEEECCCCCCCCCEEEECEEEEECCCCCCCCCHHHHCCCEEEEEHHHHHHHHHHHHH
YYLMSRGLSESEATEMIVMGFVEPFTKELPMEYAVELNRLISYEMEGSVG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure 
AEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSAVKGEPEWMLEFRLKSY
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHH
ETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPEKIKETFERIGIPEAERAY
HHHHHCCHHHHCCCHHHCCHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
LAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPDLFKQYFAKLVPPTDNKLAAL
HCCCCCCCCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
NSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFERTLIIVDEGASVHYVEGCTAPT
HHHHCCCCEEEEECCCCEEEECHHEEEEECCCCCCCEEEEEEEEECCCCEEEEECCCCCC
YSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNLVTKRAKAQKDATVEWIDGNLGAKT
CCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCE
TMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGAKMIHNAPHTSSSIVSKSIAKGGGKVD
EEECCEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCCEE
YRGQVTFNKNSKKSVSHIECDTIIMDDLSASDTIPFNEIHNSQVALEHEAKVSKISEEQL
EEEEEEECCCCCCCCCEEEECEEEEECCCCCCCCCHHHHCCCEEEEEHHHHHHHHHHHHH
YYLMSRGLSESEATEMIVMGFVEPFTKELPMEYAVELNRLISYEMEGSVG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]