Click here to switch to the map view.

The map label for this gene is pfkA [H]

Identifier: 225861210

GI number: 225861210

Start: 1238794

End: 1239801

Strand: Reverse

Name: pfkA [H]

Synonym: SPT_1303

Alternate gene names: 225861210

Gene position: 1239801-1238794 (Counterclockwise)

Preceding gene: 225861211

Following gene: 225861209

Centisome position: 58.7

GC content: 44.44

Gene sequence:

>1008_bases
ATGAAACGTATTGCTGTTTTGACTAGTGGCGGAGACGCACCTGGTATGAACGCTGCCATCCGTGCAGTTGTTCGTCAAGC
AATTTCAGAAGGAATGGAAGTGTTTGGTATCTATGACGGATATGCTGGTATGGTTGCCGGTGAAATTCATCCCCTAGATG
CAGCTTCAGTAGGGGACATCATTTCTCGTGGTGGTACTTTTCTTCACTCAGCTCGTTATCCAGAGTTCGCTCAACTTGAA
GGGCAACTTAAAGGGATTGAGCAATTGAAAAAACACGGAATTGAAGGTGTAGTTGTTATCGGTGGTGACGGATCTTACCA
CGGCGCTATGCGTTTGACTGAACATGGCTTCCCAGCTATTGGTCTTCCAGGTACAATCGATAACGATATCGTTGGTACTG
ACTTTACAATCGGTTTTGACACAGCGGTTACTACTGCCATGGACGCTATCGATAAGATTCGTGATACATCATCAAGTCAC
CGTCGTACTTTTGTAATCGAAGTTATGGGACGTAACGCTGGTGATATCGCTCTTTGGGCTGGTATTGCAACTGGTGCTGA
TGAAATCATCATCCCTGAAGCAGGCTTCAAGATGGAAGATATCGTAGCAAGCATCAAAGCTGGTTATGAATGTGGTAAAA
AACACAATATCATCGTCTTAGCTGAAGGTGTGATGTCAGCGGCTGAATTTGGTCAAAAACTTAAAGAAGCTGGAGATACA
AGCGACCTTCGTGTAACAGAACTTGGACATATTCAACGTGGTGGTTCTCCAACTGCGCGTGACCGTGTTTTGGCGTCACG
TATGGGTGCACATGCTGTTAAACTTCTTAAAGAAGGTATCGGTGGTGTTGCGGTTGGTATTCGTAACGAAAAAATGGTTG
AAAATCCAATTCTTGGTACTGCAGAAGAAGGGGCATTGTTTAGCCTTACTGCAGAAGGTAAGATTGTGGTTAACAACCCG
CACAAAGCCGATATTGAGCTATCTAGCTTGAATAAGAGCTTGTCATAA

Upstream 100 bases:

>100_bases
AAAACGATTTATCGCTAAAAATACGGAAAATAGAAGAATTTTAAAATCAAATGTGATATAATCAAGAAGAATGTTAAAAG
AAAAAGGAGCATAAACCAAT

Downstream 100 bases:

>100_bases
TTTACAATTTAGTTAATAAGACCAAAAAGGTCATATATATAAAGGAGTCACAAAAATCATGAACAAACGTGTAAAGATCG
TTGCAACTTTGGGTCCTGCG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGMVAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLE
GQLKGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNIIVLAEGVMSAAEFGQKLKEAGDT
SDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLLKEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNP
HKADIELSSLNKSLS

Sequences:

>Translated_335_residues
MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGMVAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLE
GQLKGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNIIVLAEGVMSAAEFGQKLKEAGDT
SDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLLKEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNP
HKADIELSSLNKSLS
>Mature_335_residues
MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGMVAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLE
GQLKGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNIIVLAEGVMSAAEFGQKLKEAGDT
SDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLLKEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNP
HKADIELSSLNKSLS

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=312, Percent_Identity=43.5897435897436, Blast_Score=212, Evalue=3e-55,
Organism=Homo sapiens, GI48762920, Length=314, Percent_Identity=42.6751592356688, Blast_Score=204, Evalue=8e-53,
Organism=Homo sapiens, GI266453768, Length=312, Percent_Identity=41.9871794871795, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI266453748, Length=312, Percent_Identity=41.9871794871795, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI4505749, Length=312, Percent_Identity=41.9871794871795, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI266453619, Length=334, Percent_Identity=40.1197604790419, Blast_Score=203, Evalue=2e-52,
Organism=Escherichia coli, GI1790350, Length=303, Percent_Identity=58.4158415841584, Blast_Score=340, Evalue=8e-95,
Organism=Caenorhabditis elegans, GI25147584, Length=343, Percent_Identity=39.9416909620991, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17558788, Length=310, Percent_Identity=36.1290322580645, Blast_Score=177, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI72003601, Length=288, Percent_Identity=32.9861111111111, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321679, Length=337, Percent_Identity=39.4658753709199, Blast_Score=202, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6323861, Length=343, Percent_Identity=37.6093294460641, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI28573326, Length=338, Percent_Identity=40.8284023668639, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17647809, Length=339, Percent_Identity=40.7079646017699, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24652337, Length=338, Percent_Identity=40.8284023668639, Blast_Score=218, Evalue=3e-57,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35175; Mature: 35175

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGMVAGEIHPLDAASVGDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCCCHHHHHH
ISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAI
HHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSHRRTFVIEVMGRNAGDIALWA
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEE
GIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNIIVLAEGVMSAAEFGQKLKEAGDT
EECCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC
SDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLLKEGIGGVAVGIRNEKMVENPILGT
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCC
AEEGALFSLTAEGKIVVNNPHKADIELSSLNKSLS
CCCCCEEEEECCCEEEECCCCCCCEEHHHHCCCCC
>Mature Secondary Structure
MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGMVAGEIHPLDAASVGDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCCCHHHHHH
ISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGGDGSYHGAMRLTEHGFPAI
HHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSHRRTFVIEVMGRNAGDIALWA
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEE
GIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNIIVLAEGVMSAAEFGQKLKEAGDT
EECCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC
SDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLLKEGIGGVAVGIRNEKMVENPILGT
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCC
AEEGALFSLTAEGKIVVNNPHKADIELSSLNKSLS
CCCCCEEEEECCCEEEECCCCCCCEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA