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The map label for this gene is infC

Identifier: 225861154

GI number: 225861154

Start: 1185553

End: 1186110

Strand: Reverse

Name: infC

Synonym: SPT_1244

Alternate gene names: 225861154

Gene position: 1186110-1185553 (Counterclockwise)

Preceding gene: 225861155

Following gene: 225861153

Centisome position: 56.16

GC content: 39.25

Gene sequence:

>558_bases
TTGTTTTTCTCTAATAAAACAAAAGAGGTGAAAACCATAGCAAAGCAAGACTTATTCATCAATGATGAGATTCGTGTACG
TGAAGTTCGCTTGATTGGTCTTGAGGGAGAACAGCTAGGCATCAAGCCACTCAGTGAAGCGCAAGCTTTGGCTGATAACG
CTAATGTTGACCTAGTATTGATTCAACCCCAAGCCAAACCGCCTGTTGCAAAAATTATGGACTACGGTAAGTTCAAATTT
GAGTACCAGAAGAAGCAAAAAGAACAACGTAAAAAACAAAGCGTTGTTACTGTGAAAGAAGTTCGTCTAAGTCCAACAAT
TGACAAGGGTGACTTTGATACAAAACTTCGCAATGCACGTAAGTTCCTTGAAAAAGGAAATAAAGTTAAGGTATCTATTC
GCTTTAAGGGTCGTATGATTACCCATAAAGAGATTGGTGCAAAAGTTTTAGCCGAGTTTGCTGAAGCAACTCAAGATATT
GCCATCATCGAACAACGTGCTAAAATGGATGGACGTCAAATGTTCATGCAATTGGCGCCAGCGACTGACAAAAAATAA

Upstream 100 bases:

>100_bases
GCTTCTCGCCTTGTGACATTAAGTTGCCTGGCCCTACGGATGAAAAGTTTCGAAGAAACGCTATCATAACGTGCGGGCTT
GTATATTTACGAGTCCGCTA

Downstream 100 bases:

>100_bases
TTGTCAGAAAGTTAAATAAAGGAGAAAAAATCATGCCAAAACAAAAAACACACCGCGCATCAGCTAAACGTTTCAAACGT
ACAGGTTCTGGTGGACTTAA

Product: translation initiation factor IF-3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MFFSNKTKEVKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADNANVDLVLIQPQAKPPVAKIMDYGKFKF
EYQKKQKEQRKKQSVVTVKEVRLSPTIDKGDFDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDI
AIIEQRAKMDGRQMFMQLAPATDKK

Sequences:

>Translated_185_residues
MFFSNKTKEVKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADNANVDLVLIQPQAKPPVAKIMDYGKFKF
EYQKKQKEQRKKQSVVTVKEVRLSPTIDKGDFDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDI
AIIEQRAKMDGRQMFMQLAPATDKK
>Mature_185_residues
MFFSNKTKEVKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADNANVDLVLIQPQAKPPVAKIMDYGKFKF
EYQKKQKEQRKKQSVVTVKEVRLSPTIDKGDFDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDI
AIIEQRAKMDGRQMFMQLAPATDKK

Specific function: IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins

COG id: COG0290

COG function: function code J; Translation initiation factor 3 (IF-3)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-3 family

Homologues:

Organism=Escherichia coli, GI1788012, Length=162, Percent_Identity=53.0864197530864, Blast_Score=156, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IF3_STRP2 (Q04KW9)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816329.1
- ProteinModelPortal:   Q04KW9
- STRING:   Q04KW9
- EnsemblBacteria:   EBSTRT00000020542
- GeneID:   4441324
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0847
- eggNOG:   COG0290
- GeneTree:   EBGT00050000028388
- HOGENOM:   HBG744268
- OMA:   APKAPIN
- ProtClustDB:   PRK00028
- GO:   GO:0005737
- HAMAP:   MF_00080
- InterPro:   IPR019813
- InterPro:   IPR001288
- InterPro:   IPR019815
- InterPro:   IPR019814
- Gene3D:   G3DSA:3.10.20.80
- Gene3D:   G3DSA:3.30.110.10
- PANTHER:   PTHR10938
- TIGRFAMs:   TIGR00168

Pfam domain/function: PF00707 IF3_C; PF05198 IF3_N; SSF54364 IF3; SSF55200 IF3

EC number: NA

Molecular weight: Translated: 21165; Mature: 21165

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS00938 IF3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFSNKTKEVKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADNANVDLVL
CCCCCCHHHHHHHHHHCCEECCCEEEEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEE
IQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGDFDTKLRNAR
ECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCEEHHHEEECCCCCCCCCHHHHHHHH
KFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKMDGRQMFMQLAP
HHHHCCCEEEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
ATDKK
CCCCC
>Mature Secondary Structure
MFFSNKTKEVKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADNANVDLVL
CCCCCCHHHHHHHHHHCCEECCCEEEEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEE
IQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGDFDTKLRNAR
ECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCEEHHHEEECCCCCCCCCHHHHHHHH
KFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKMDGRQMFMQLAP
HHHHCCCEEEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
ATDKK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA