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The map label for this gene is nudF [H]

Identifier: 225861126

GI number: 225861126

Start: 1159103

End: 1159648

Strand: Reverse

Name: nudF [H]

Synonym: SPT_1215

Alternate gene names: 225861126

Gene position: 1159648-1159103 (Counterclockwise)

Preceding gene: 225861127

Following gene: 225861125

Centisome position: 54.9

GC content: 40.84

Gene sequence:

>546_bases
ATGGAGTTTGAAGAAAAAACGCTTAGCCGAAAAGAAATCTATCAAGGACCAATATTTAAACTGGTCCAAGATCAGGTTGA
ATTACCAGAAGGCAAGGGAACTGCCCAACGGGATTTGATTTTCCACAATGGGGCTGTCTGTGTTTTAGCAGTAACGGATG
AACAAAAACTTATCTTGGTCAAGCAGTACCGCAAAGCTATCGAGGCAGTCTCTTACGAAATTCCAGCCGGAAAATTGGAA
GTAGGAGAAAACACAGCCCCTGTGGCAGCTGCCCTTCGTGAATTAGAGGAAGAAACAGCCTATACAGGGAAATTAGAACT
CTTGTACGATTTTTATTCAGCTATTGGCTTTTGTAATGAGAAGTTAAAACTATATTTAGCAAGCGATTTAACAAAAGTGG
AAAATCCGCGTCCGCAGGATGAGGATGAAACCTTGGAAGTCCTTGAAGTGAGCTTAGAAGAAGCGAAAGAATTAATCCAA
TCAGGTCATATTTGTGATGCCAAGACAATTATGGCTGTTCAGTATTGGGAGTTGCAGAAAAAATAG

Upstream 100 bases:

>100_bases
GCCAGCAGATGCTATTGCTATTGGTCGCGGTCGTCAGATCAATAAAGACGAATATGCAACACGTCTTCCTCATCATCCTA
AGAACCAGTAGGAGCCTATC

Downstream 100 bases:

>100_bases
AGGAGGTCAGTATGGGTAAATCTTTATTAACGGATGAAATGATTGAAAGAGCTAATAGAGGCGAAAAAATTTCAGGTCCT
CCTTTGCTAGATGATAATGA

Product: ADP-ribose pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNGAVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLE
VGENTAPVAAALRELEEETAYTGKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
SGHICDAKTIMAVQYWELQKK

Sequences:

>Translated_181_residues
MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNGAVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLE
VGENTAPVAAALRELEEETAYTGKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
SGHICDAKTIMAVQYWELQKK
>Mature_181_residues
MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNGAVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLE
VGENTAPVAAALRELEEETAYTGKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
SGHICDAKTIMAVQYWELQKK

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6319587, Length=150, Percent_Identity=33.3333333333333, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 20515; Mature: 20515

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNGAVCVLAVTDEQKLILV
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHCEEEECCCEEEEEECCCCCCHHH
KQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYTGKLELLYDFYSAIGFCNE
HHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCH
KLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQSGHICDAKTIMAVQYWELQK
HHEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
K
C
>Mature Secondary Structure
MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNGAVCVLAVTDEQKLILV
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHCEEEECCCEEEEEECCCCCCHHH
KQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYTGKLELLYDFYSAIGFCNE
HHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCH
KLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQSGHICDAKTIMAVQYWELQK
HHEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]