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The map label for this gene is iscS1 [H]

Identifier: 225861059

GI number: 225861059

Start: 1073768

End: 1074883

Strand: Reverse

Name: iscS1 [H]

Synonym: SPT_1139

Alternate gene names: 225861059

Gene position: 1074883-1073768 (Counterclockwise)

Preceding gene: 225861060

Following gene: 225861058

Centisome position: 50.89

GC content: 42.65

Gene sequence:

>1116_bases
TTGATTTATTTGGACAATGCTGCAACGACTCCTATGTCAGCAGTTGCTATTTCAGCTATGACCAAGGTTATGCAAGAAAC
CCACGGAAATCCTTCTAGTATTCATGGTCATGGTCGTCAAGCTGGCAAACTCTTGCGAGAAGCCCGTCAGGAACTAGCCC
AGTTACTAAGGACAAAACCTCAACATATCTTTTTCACTTCTGGTGGGACTGAAGGCAATAATACTACCATCATTGGCTAC
TGTCTTCGTCACCAAGAACAAGGAAAACATATCATCACAACTGCCATCGAGCACCATGCTGTCCTTGAAACAATTGATTA
CTTGGTTCAACACTTTGGGTTTGAAGCAACCATTATCCAGCCAGAAAATCAAGAAATCACAGCCCAGCAAATTCAAAAGG
CTTTACGTGACGATACGATTTTGGTTTCTACTATGTTTGCCAATAATGAGACAGGAAACCTACTTCCCATCGCTGAAATT
GGCCAAATACTCAAGCAACACCCTGCTGCCTATCATGTTGATGCAGTTCAGGCTATTGGTAAAATCCCAATTCATTCAGA
AGAATTGGGCATTGATTTTCTCACTGCTTCTGCCCACAAATTCCATGGTCCTAAGGGAATCGGTTTTCTCTACGCATCTA
GCATGGACTTTGATTCCTATCTACATGGCGGAGATCAGGAACAGAAAAAACGTGCAGGAACTGAAAATCTGCCTGCCATC
GTAGGCATGGTTGCAGCCCTAAAAGAAGACCTAGAAAAACAAGAAGAACATTTTCAACATGTACAAAATCTAGAAACTGC
CTTTCTGGCAGAGCTGGAGGGCATTCAGTATTACTTGAATAGAGGAAAACATCATCTCCCTTATGTTCTCAATATTGGAT
TTCCTGGTCAGAAAAATGACCTCTTACTCCTTCGGCTAGATTTAGCTGGAATTTCAATCTCTACTGGCTCAGCCTGTACT
GCAGGCGTTGTCCAATCCAGCCATGTTCTTAAAGCCATGTATGGCGCAAATTCAGAACGCTTGAAGGAATCCCTTCGCAT
CAGTTTGTCGCCACAAAATACCGTTGAAGACCTACAAACCCTCGCAAAAACCTTAAAAGAAATTATCGGAGGTTAG

Upstream 100 bases:

>100_bases
CACTGCTAGTGAGTTAATTGGTGATGCTATCGTCCGTATTCACGAAAGAAAACCAGTCAGCCCACTCTTTGCCTACAATA
AAAAGAAATAAGGTGATTCT

Downstream 100 bases:

>100_bases
CCATATGGCATTTGAAAAAATCATTCAGTTAAAAAATTGTCGTTACGATTACACTCTTAGCCCTTCTGTTAAAAAATTCA
CCCTCAAAGATAACACCTTT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKLLREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGY
CLRHQEQGKHIITTAIEHHAVLETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFDSYLHGGDQEQKKRAGTENLPAI
VGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQYYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACT
AGVVQSSHVLKAMYGANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG

Sequences:

>Translated_371_residues
MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKLLREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGY
CLRHQEQGKHIITTAIEHHAVLETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFDSYLHGGDQEQKKRAGTENLPAI
VGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQYYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACT
AGVVQSSHVLKAMYGANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG
>Mature_371_residues
MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKLLREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGY
CLRHQEQGKHIITTAIEHHAVLETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFDSYLHGGDQEQKKRAGTENLPAI
VGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQYYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACT
AGVVQSSHVLKAMYGANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=376, Percent_Identity=32.9787234042553, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI156713448, Length=409, Percent_Identity=33.7408312958435, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI48994898, Length=381, Percent_Identity=38.5826771653543, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1787970, Length=406, Percent_Identity=26.3546798029557, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789175, Length=394, Percent_Identity=24.6192893401015, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=367, Percent_Identity=34.3324250681199, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17533177, Length=329, Percent_Identity=32.8267477203647, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6319831, Length=375, Percent_Identity=33.8666666666667, Blast_Score=205, Evalue=1e-53,
Organism=Drosophila melanogaster, GI20129463, Length=376, Percent_Identity=32.7127659574468, Blast_Score=197, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40735; Mature: 40735

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKLLREARQELAQLLRTKP
CEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
QHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVLETIDYLVQHFGFEATIIQ
CEEEEECCCCCCCCCEEEEEEEHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEC
PENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEIGQILKQHPAAYHVDAVQAIG
CCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHC
KIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFDSYLHGGDQEQKKRAGTENLPAI
CCCCCCHHCCCEEEECCHHHCCCCCCCEEEEECCCCHHHHHCCCCHHHHHHCCCCCCHHH
VGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQYYLNRGKHHLPYVLNIGFPGQKND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
LLLLRLDLAGISISTGSACTAGVVQSSHVLKAMYGANSERLKESLRISLSPQNTVEDLQT
EEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCHHHHHHHH
LAKTLKEIIGG
HHHHHHHHHCC
>Mature Secondary Structure
MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKLLREARQELAQLLRTKP
CEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
QHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVLETIDYLVQHFGFEATIIQ
CEEEEECCCCCCCCCEEEEEEEHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEC
PENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEIGQILKQHPAAYHVDAVQAIG
CCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHC
KIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFDSYLHGGDQEQKKRAGTENLPAI
CCCCCCHHCCCEEEECCHHHCCCCCCCEEEEECCCCHHHHHCCCCHHHHHHCCCCCCHHH
VGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQYYLNRGKHHLPYVLNIGFPGQKND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
LLLLRLDLAGISISTGSACTAGVVQSSHVLKAMYGANSERLKESLRISLSPQNTVEDLQT
EEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCHHHHHHHH
LAKTLKEIIGG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]