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The map label for this gene is trmE [H]

Identifier: 225861000

GI number: 225861000

Start: 1011984

End: 1013357

Strand: Reverse

Name: trmE [H]

Synonym: SPT_1069

Alternate gene names: 225861000

Gene position: 1013357-1011984 (Counterclockwise)

Preceding gene: 225861001

Following gene: 225860996

Centisome position: 47.98

GC content: 44.4

Gene sequence:

>1374_bases
ATGATTACACGTGAATTTGATACCATCGCTGCTATCTCTACTCCACTAGGTGAAGGAGCTATTGGTATTGTCCGCCTGAG
CGGAACAGACAGTTTTGCTATTGCGCAAAAGATTTTTAAAGGAAAAGACTTGAACAAGGTTGCCAGCCACACTCTCAACT
ACGGTCACATTATTGATCCTCTGACTGGTAAAGTCATGGACGAGGTTATGGTTGGGGCTATGAAGTCTCCAAAGACCTTC
ACTCGTGAGGATATTATCGAGATTAACACCCACGGTGGTATTGCGGTGACCAATGAGATTCTCCAGCTAGCTATCCGTGA
AGGAGCTCGGTTGGCAGAACCTGGTGAATTTACCAAACGTGCCTTTTTAAACGGTCGCGTAGATTTGACACAGGCCGAGG
CGGTGATGGATATCATCCGAGCCAAGACAGACAAGGCCATGAACATCGCGGTCAAACAACTTGATGGTTCCCTTTCTGAT
CTTATCAATAATATCCGTCAAGAAATCCTTAACACACTTGCCCAAGTTGAGGTCAATATCGACTATCCTGAGTATGACGA
TGTTGAGGAAGCCACTACTGCTGTTGTCCGAGAGAAGACAATGGAGTTTGAGCAATTACTAACCAAGCTCCTTAGGACAG
CACGTCGTGGTAAAATCCTTCGTGAAGGAATTTCAACGGCTATCATTGGACGTCCCAACGTTGGGAAATCAAGCCTTCTC
AACAACCTCTTGCGTGAGGACAAGGCTATCGTAACAGATATCGCTGGGACAACACGAGATGTCATCGAAGAGTACGTCAA
CATCAATGGTGTACCTCTCAAATTGATTGATACAGCCGGTATTCGTGAAACAGATGACATAGTGGAACAAATCGGTGTTG
AGCGTTCGAAAAAAGCCCTCAAGGAAGCCGACTTGGTTCTACTAGTGCTAAATGCCAGTGAACCACTGACTGCGCAAGAC
AGACAACTTCTTGAAATTAGCCAAGATACCAATCGCATTATTCTACTTAATAAAACCGACCTGCCAGAAACGATTGAAAC
TTCGAAACTACCTGAAGACGTTATCCGTATTTCAGTCCTTAAAAACCAAAACATCGATAAGATTGAAGAGCGAATCAACA
ACCTCTTCTTTGAAAATGCTGGTTTGGTTGAGCAAGATGCTACCTACTTGTCAAACGCCCGTCACATTTCCTTGATTGAG
AAGGCCGTTGAAAGCCTACAAGCTGTTAACCAAGGTCTTGAACTAGGGATGCCAGTTGACTTGCTTCAAGTTGACTTGAC
CCGTACTTGGGAAATTCTAGGAGAAATCACTGGAGATGCTGCTCCAGATGAACTCATCACCCAACTCTTTAGCCAATTCT
GTTTAGGAAAATAA

Upstream 100 bases:

>100_bases
CTCCAAGTAGCACCCATTGAAGAAATAGTCTAAATTTGTTACAATTTGAAAAGAGACTTGGAAAATTCCAAGAAAAGAGC
AATTAATTAAAGGAAATATT

Downstream 100 bases:

>100_bases
GAAAAATCCATGATCCTTCATTCGGTCATGGATTTTATTGTCTTTATTAGTAATCTGGTCTTAAGACCCCTGTTACAGTT
GCCTTAGTTGCTTCGTAGTC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDLNKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTF
TREDIIEINTHGGIAVTNEILQLAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGKILREGISTAIIGRPNVGKSSLL
NNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLIDTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQD
RQLLEISQDTNRIILLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSNARHISLIE
KAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK

Sequences:

>Translated_457_residues
MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDLNKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTF
TREDIIEINTHGGIAVTNEILQLAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGKILREGISTAIIGRPNVGKSSLL
NNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLIDTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQD
RQLLEISQDTNRIILLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSNARHISLIE
KAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
>Mature_457_residues
MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDLNKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTF
TREDIIEINTHGGIAVTNEILQLAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGKILREGISTAIIGRPNVGKSSLL
NNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLIDTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQD
RQLLEISQDTNRIILLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSNARHISLIE
KAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=34.468085106383, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=33.9869281045752, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=32.8657314629259, Blast_Score=229, Evalue=3e-60,
Organism=Escherichia coli, GI2367268, Length=460, Percent_Identity=34.1304347826087, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=41.8032786885246, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=463, Percent_Identity=31.317494600432, Blast_Score=193, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=31.0344827586207, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=30.6553911205074, Blast_Score=202, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50517; Mature: 50517

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDLNKVASHTLNYGHIIDP
CCCCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
LTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQLAIREGARLAEPGEFTKR
HHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSDLINNIRQEILNTLAQVEVNI
HHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEC
DYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGKILREGISTAIIGRPNVGKSSLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHH
NNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLIDTAGIRETDDIVEQIGVERSKKAL
HHHHHHCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHH
KEADLVLLVLNASEPLTAQDRQLLEISQDTNRIILLNKTDLPETIETSKLPEDVIRISVL
HHCCEEEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHH
KNQNIDKIEERINNLFFENAGLVEQDATYLSNARHISLIEKAVESLQAVNQGLELGMPVD
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
LLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDLNKVASHTLNYGHIIDP
CCCCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
LTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQLAIREGARLAEPGEFTKR
HHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSDLINNIRQEILNTLAQVEVNI
HHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEC
DYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGKILREGISTAIIGRPNVGKSSLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHH
NNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLIDTAGIRETDDIVEQIGVERSKKAL
HHHHHHCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHH
KEADLVLLVLNASEPLTAQDRQLLEISQDTNRIILLNKTDLPETIETSKLPEDVIRISVL
HHCCEEEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHH
KNQNIDKIEERINNLFFENAGLVEQDATYLSNARHISLIEKAVESLQAVNQGLELGMPVD
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
LLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA