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The map label for this gene is pepT [H]

Identifier: 225860987

GI number: 225860987

Start: 998743

End: 999966

Strand: Reverse

Name: pepT [H]

Synonym: SPT_1055

Alternate gene names: 225860987

Gene position: 999966-998743 (Counterclockwise)

Preceding gene: 225860995

Following gene: 225860982

Centisome position: 47.34

GC content: 42.48

Gene sequence:

>1224_bases
ATGACTTATCCAAATCTCTTGGACCACTTCTTAACCTACGTTAAGGTCAACACGCGCTCTGATGAACACTCTACTACTAC
TCCAAGTACACAGAGTCAGGTTGACTTCGCAACAAATGTCCTAATTCCTGAAATGAAACGTGTTGGACTGCAAAATGTTT
ACTATCTACCGAATGGTTTTGCTATTGGAACCTTGCCAGCCAACGATCCGTCTTTAACACGTAAGATTGGTTTTATATCG
CACATGGATACTGCTGATTTTAATGCTGAAGGAGTCAATCCACAGGTAATTGAAAACTACGATGGTGGTGTGATTGAACT
AGGGAATTCTGGTTTCAAACTCGATCCAGCTGACTTCAAGAGTCTTGAAAAATATCCAGGACAAACGCTCATCACAACAG
ATGGAACAACCTTGCTAGGTGCTGATGACAAGTCAGGAATTGCTGAAATTATGACAGCCATTGAATATCTAACTGCTCAT
CCTGAAATTAAGCACTGTGAGATTCGTGTTGGTTTTGGTCCAGATGAAGAAATCGGTGTTGGTGCCAATAAATTTGATGC
AGAAGATTTTGATGTGGATTTTGCCTACACTGTTGACGGTGGTCCACTAGGTGAACTTCAGTACGAGACTTTCTCAGCCG
CTGGTGCTGAATTGCATTTCCAAGGCCGTAATGTCCACCCTGGTACTGCCAAAGGGCAGATGGTCAATGCCCTTCAGCTA
GCAATTGATTTTCATAATCAACTTCCAGAAAATGACCGACCTGAGTTAACTGAAGGTTACCAAGGTTTTTACCATCTAAT
GGATGTGACAGGTAGTGTTGAGGAGACGCGTGCAAGCTACATCATTCGTGATTTTGAAAAAGATGCCTTTGAAGCGCGTA
AAGCATCCATGCAATCTATCGCTGATAAGATGAATGAAGAACTTGGGAGCGACCGTGTCACTCTCAACTTGACAGACCAG
TACTACAATATGAAAGAAGTCATTGAAAAAGATATGACTCCAATTACCATTGCTAAAGCCGTTATGGAAGATCTAGGTAT
CACGCCTATTATCGAACCAATCCGGGGTGGAACAGACGGCTCTAAGATTTCCTTTATGGGAATCCCAACTCCGAATATCT
TTGCAGGTGGCGAAAATATGCACGGACGTTTTGAATACGTTAGCCTTCAGACTATGGAACGTGCAGTTGATACCATCATT
GGCATCGTAGCTTATAAAGGCTAA

Upstream 100 bases:

>100_bases
GAATTTGAATTATTATATTTTTTCAAAATTCATAAAATTTCTCAAATCATATTTTTCCGAAGTTTTGTATTCTATTTTAT
GATAAAAAAGGAGTTTTTTT

Downstream 100 bases:

>100_bases
AAAGACGAGGTGGCTCAGCTACTTCGCCTTTCTTTTTATTCTACTGGTTTTTCTTGATTTCCAGTAGTTGTAGAAGATTC
TGTTGTTTCATTTTCTGAAG

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MTYPNLLDHFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPEMKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFIS
HMDTADFNAEGVNPQVIENYDGGVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAELHFQGRNVHPGTAKGQMVNALQL
AIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEETRASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQ
YYNMKEVIEKDMTPITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTMERAVDTII
GIVAYKG

Sequences:

>Translated_407_residues
MTYPNLLDHFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPEMKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFIS
HMDTADFNAEGVNPQVIENYDGGVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAELHFQGRNVHPGTAKGQMVNALQL
AIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEETRASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQ
YYNMKEVIEKDMTPITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTMERAVDTII
GIVAYKG
>Mature_406_residues
TYPNLLDHFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPEMKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISH
MDTADFNAEGVNPQVIENYDGGVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAHP
EIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAELHFQGRNVHPGTAKGQMVNALQLA
IDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEETRASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQY
YNMKEVIEKDMTPITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTMERAVDTIIG
IVAYKG

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=400, Percent_Identity=49.75, Blast_Score=380, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 44801; Mature: 44670

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYPNLLDHFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPEMKRVGLQNVYYLPNGF
CCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCE
AIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDGGVIELGNSGFKLDPADFK
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCEECHHHHH
SLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAHPEIKHCEIRVGFGPDEEIGV
HHHHCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
GANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAELHFQGRNVHPGTAKGQMVNALQL
CCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHH
AIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEETRASYIIRDFEKDAFEARKASMQSI
EEHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTPITIAKAVMEDLGITPIIEPIRGGTDG
HHHHHHHHCCCEEEEEECHHHCCHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHCCCCCC
SKISFMGIPTPNIFAGGENMHGRFEYVSLQTMERAVDTIIGIVAYKG
CEEEEEECCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
TYPNLLDHFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPEMKRVGLQNVYYLPNGF
CCHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCE
AIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDGGVIELGNSGFKLDPADFK
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCEECHHHHH
SLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAHPEIKHCEIRVGFGPDEEIGV
HHHHCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
GANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAELHFQGRNVHPGTAKGQMVNALQL
CCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHH
AIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEETRASYIIRDFEKDAFEARKASMQSI
EEHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTPITIAKAVMEDLGITPIIEPIRGGTDG
HHHHHHHHCCCEEEEEECHHHCCHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHCCCCCC
SKISFMGIPTPNIFAGGENMHGRFEYVSLQTMERAVDTIIGIVAYKG
CEEEEEECCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA