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The map label for this gene is yjfH [C]

Identifier: 225860774

GI number: 225860774

Start: 772809

End: 773537

Strand: Reverse

Name: yjfH [C]

Synonym: SPT_0818

Alternate gene names: 225860774

Gene position: 773537-772809 (Counterclockwise)

Preceding gene: 225860775

Following gene: 225860766

Centisome position: 36.62

GC content: 42.8

Gene sequence:

>729_bases
ATGAAAACAAATGATATTGTCTATGGCGTCCACGCCGTTACCGAAGCCCTCCTTGCAAATACAGGAAACAAACTCTACCT
CCAAGAAGATCTCCGAGGTAAGAATGTTGAGAAAGTCAAGGAACTAGCTACAGAAAAGAAGGTGTCCATTTCTTGGACAT
CAAAAAAATCTCTCTCTGAGATTACTGAAGGTGCTGTTCATCAAGGTTTTGTTCTACGAGTGTCTGAATTTGCCTATAGC
GAGCTAGATTACATCCTTGCAAAAACACGCCAAGAAGAAAATCCACTTCTATTGATTCTAGATGGTCTAACCGATCCCCA
TAATCTAGGTTCTATCTTGCGAACAGCCGATGCGACCAATGTTTCAGGTGTCATCATTCCCAAGCACCGTGCTGTCGGAG
TAACTCCTGTCGTTGCCAAAACAGCCACAGGTGCTATTGAACACGTTCCAATTGCCCGAGTGACCAACCTCAGTCAAACC
TTAGATAAACTTAAGGATGAAGGTTTCTGGACCTTTGGAACGGATATGAACGGTACTCCTTGCCACAAGTGGAATACAAA
AGGGAAAATCGCCCTCATCATTGGAAATGAAGGAAAAGGTATCTCTAGCAACATCAAAAAACAAGTCGATGAGATGATTA
CCATTCCGATGAATGGACATGTTCAAAGCCTTAATGCCAGTGTTGCTGCGGCCATTCTCATGTACGAAGTTTTCCGAAAT
AGACTATAA

Upstream 100 bases:

>100_bases
GCATCAATGAAAAAGACCCATTTGCAGTTAAAGATGGTTTACTGATTCTTGAATAAAGAAAATCCCGTTGCAAGACGGGG
TTTTGTGTTATAATAGAGGC

Downstream 100 bases:

>100_bases
AAAGTTTCCAGTCATCTGATTGGAAACTTTTTTATAATTAACTATGTTCTGTAATGAATTTATAGGCTTCTTGACCAGCG
ATAGCTCCATCTCCAACCGC

Product: RNA methyltransferase, TrmH family, group 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELATEKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYS
ELDYILAKTRQEENPLLLILDGLTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMNGHVQSLNASVAAAILMYEVFRN
RL

Sequences:

>Translated_242_residues
MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELATEKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYS
ELDYILAKTRQEENPLLLILDGLTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMNGHVQSLNASVAAAILMYEVFRN
RL
>Mature_242_residues
MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELATEKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYS
ELDYILAKTRQEENPLLLILDGLTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMNGHVQSLNASVAAAILMYEVFRN
RL

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=263, Percent_Identity=26.9961977186312, Blast_Score=91, Evalue=9e-19,
Organism=Escherichia coli, GI1790623, Length=247, Percent_Identity=40.080971659919, Blast_Score=171, Evalue=3e-44,
Organism=Escherichia coli, GI1790083, Length=167, Percent_Identity=30.5389221556886, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788935, Length=146, Percent_Identity=29.4520547945205, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17555908, Length=257, Percent_Identity=26.0700389105058, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324775, Length=286, Percent_Identity=28.3216783216783, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26491; Mature: 26491

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELATEKKVSISWTSKKSLSE
CCCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCEEEEEECCHHHHHH
ITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDGLTDPHNLGSILRTADATN
HHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCC
VSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQTLDKLKDEGFWTFGTDMNGTP
CCEEEEECCCCCCCCHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
CHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMNGHVQSLNASVAAAILMYEVFRN
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHH
RL
CC
>Mature Secondary Structure
MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELATEKKVSISWTSKKSLSE
CCCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCEEEEEECCHHHHHH
ITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDGLTDPHNLGSILRTADATN
HHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCC
VSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQTLDKLKDEGFWTFGTDMNGTP
CCEEEEECCCCCCCCHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
CHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMNGHVQSLNASVAAAILMYEVFRN
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHH
RL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA