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The map label for this gene is insK [H]

Identifier: 225860577

GI number: 225860577

Start: 562025

End: 562858

Strand: Reverse

Name: insK [H]

Synonym: SPT_0603

Alternate gene names: 225860577

Gene position: 562858-562025 (Counterclockwise)

Preceding gene: 225860585

Following gene: 225860566

Centisome position: 26.65

GC content: 35.97

Gene sequence:

>834_bases
ATGACTGAGTTTTCGTTAGTTCTTCTTCTAAAATCCATTAAACTAGCTCGTTGGACCTACTACTATCACTTGAAACAGCT
AGACAAAACAGATAAGGACCAAGAGCTTAAAGCTGAAATTCAATCCATTTTTATCGAACACAAGGGAAATTATGCTTATC
GTCGGATTTATTTAGAACTAAGAAATCGTGCTTATCTGGTAAATCATAAAAGAGTTCAAGGCTTGATGAAAGTACTCAAT
TTACAAGCTAAAATGCGACAGAAACGAAAATATTCTTCTCATAAAGGAGACGTTGGCAAGAAGGCAGAGAATCTCATTCA
AGGCCAATTTGAAGGCTCTAAAACAATGGAAAAGTGCTATACAGATGTGACAGAATTTGCCATTCCAGCAAGTACTCAAA
AGCTTTACTTATCACCAGTTTTAGATGGCTTTAACAGCGAAATTATTGCTTTTAATCTTTCTTGTTCGCCTAATTTAGAA
CAAGTACAAACAATGTTGGAACAGGCATTCACAGAGAAGCACTACGAAAATACGATTCTCCATAGTGACCAAGGCTGGCA
ATACCAACACGATTCTTATCATCAGTTTCTTGAGGGTAAGGGAATTCAAGCATCCATGTCACGTAAGGGAAACAGCCAAG
ACAACGGTATGATGGAGTCCTTCTTTGGGATTCTGAAATCGGAAATGTTTTATGGCTATGAGAAAACATTTAAATCACTT
AATCAATTGGAACAAGCTATTGTAGACTATATTGATTACTACAACAATAAACGAATTAAGGTAAAACTAAAAGGACTTAG
CCTTGTGCAATACAGAACTAAATCCTTCGGATAA

Upstream 100 bases:

>100_bases
AGTACCTGAGAGCGGAGAATGCCATCCTAAAAAAGTTAAGAGAACTCCGATTGAAGGAGGAAAAAGAGAAAGAAGAAAGA
CAGAAATTGTTCAAGAATTA

Downstream 100 bases:

>100_bases
ATTATTTGTCTAACTTTTTGGGGTCAGTACACCTAAAACTTTGATGATATACGTTTTCTTGTGAGAATATTTACTTCATT
TTTGCCTAAAATTCAATGTT

Product: integrase core domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTEFSLVLLLKSIKLARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRIYLELRNRAYLVNHKRVQGLMKVLN
LQAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAFNLSCSPNLE
QVQTMLEQAFTEKHYENTILHSDQGWQYQHDSYHQFLEGKGIQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKTFKSL
NQLEQAIVDYIDYYNNKRIKVKLKGLSLVQYRTKSFG

Sequences:

>Translated_277_residues
MTEFSLVLLLKSIKLARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRIYLELRNRAYLVNHKRVQGLMKVLN
LQAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAFNLSCSPNLE
QVQTMLEQAFTEKHYENTILHSDQGWQYQHDSYHQFLEGKGIQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKTFKSL
NQLEQAIVDYIDYYNNKRIKVKLKGLSLVQYRTKSFG
>Mature_276_residues
TEFSLVLLLKSIKLARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRIYLELRNRAYLVNHKRVQGLMKVLNL
QAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAFNLSCSPNLEQ
VQTMLEQAFTEKHYENTILHSDQGWQYQHDSYHQFLEGKGIQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKTFKSLN
QLEQAIVDYIDYYNNKRIKVKLKGLSLVQYRTKSFG

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=253, Percent_Identity=28.0632411067194, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1789981, Length=274, Percent_Identity=46.3503649635037, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1788405, Length=282, Percent_Identity=27.6595744680851, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787263, Length=282, Percent_Identity=27.6595744680851, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786753, Length=282, Percent_Identity=27.6595744680851, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786570, Length=282, Percent_Identity=27.6595744680851, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786490, Length=282, Percent_Identity=27.6595744680851, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32500; Mature: 32368

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCC
>Mature Secondary Structure 
TEFSLVLLLKSIKLARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRIYLEL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RNRAYLVNHKRVQGLMKVLNLQAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCY
HCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
TDVTEFAIPASTQKLYLSPVLDGFNSEIIAFNLSCSPNLEQVQTMLEQAFTEKHYENTIL
HHHHHHCCCCCCHHEEHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEE
HSDQGWQYQHDSYHQFLEGKGIQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKTFKSL
ECCCCCCCCCHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NQLEQAIVDYIDYYNNKRIKVKLKGLSLVQYRTKSFG
HHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]