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The map label for this gene is dinB [H]

Identifier: 225860481

GI number: 225860481

Start: 456214

End: 457275

Strand: Reverse

Name: dinB [H]

Synonym: SPT_0497

Alternate gene names: 225860481

Gene position: 457275-456214 (Counterclockwise)

Preceding gene: 225860483

Following gene: 225860478

Centisome position: 21.65

GC content: 40.96

Gene sequence:

>1062_bases
ATGTTGATTTTTCCTTTGTTAAATGATTTGTCAAGAAAAATCATCCATATTGACATGGATGCCTTTTTTGCTGCGGTGGA
AATCAAGGATAATCCTAAACTCAGAGGAAAACCTGTCATTATTGGAAGCGACCCTCGGCAAACAGGTGGACGGGGAGTCG
TTTCTACCTGTAGCTATGAGGCTCGAGCTTTTGGTGTCCATTCTGCCATGAGTTCCAAGGAAGCTTATGAACGTTGTCCC
CAGGCCGTCTTTATCTCAGGAAATTATGAGAAATACAAAGCTGTGGGACTCCAGATTCGAGCTATTTTTAAGCGCTATAC
AGATTTGATTGAACCCATGAGCATTGACGAAGCCTATTTGGATGTGACAGAAAATAAACTCGGTATCAAGTCAGCGGTCA
AAATTGCTCGCCTCATTCAAAAAGATATCTGGCAAGAACTCCATCTAACTGCTTCTGCCGGTATTTCCTACAACAAGTTC
TTAGCTAAAATGGCGAGTGATTATCAAAAACCTCATGGTTTGACAGTGATTCTACCTGAACAGGCTGAGAATTTTCTCAA
ACAAATGGATATTTCCAAATTTCATGGAGTAGGAAAAAAGACAGTAGAACGTCTTCATCAAATGGGCGTTTTTACTGGTG
CTGATTTACTTGAAGTTCCTGAGGTGACCCTAATAGACCGTTTTGGTAGACTAGGCTATGATCTGTATCGAAAGGCTCGT
GGCATTCACAACTCTCCAGTCAAATCCAATCGCATCCGTAAATCAATCGGCAAGGAGAAAACCTACGGGAAGATTCTCCG
TGCTGAGGAAGATATCAAAAAAGAGCTGACTATTCTATCAGAAAAAGTCGCTCTCAATCTACATCAACAAGAAAAAGCTG
GAAAAATTGTCATTTTGAAAATCCGCTACGAGGACTTTTCAACTCTTACCAAACGAAAAAGTCTTGCTCAAAAAACACAA
GATGCTAGTCAGATAAGCCAAATAGCCCTGCAACTCTATGAAGAATTAAGCGAGAAAGAAAGAGGTGTCCGCCTATTGGG
GATTACCCTGACTGGATTTTAA

Upstream 100 bases:

>100_bases
AGGAAGCTAGCCGCAGATTTCTCAAAACACTGTTTTGAGGTTGCAGATAGAGCTGACGTGGTTTGAAGAGATTTTCGAAG
AGTATAAAAAGGTGCTAGGC

Downstream 100 bases:

>100_bases
AGCTCAATGAAAATCAAAGAGCAAACTAGGAAGCTAGTCGCAGGCTGCTCAAAACACTGTTTTGAGGTTGCAGATAAAGC
TGACGCGGTTTGAAGAAATT

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSDPRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCP
QAVFISGNYEKYKAVGLQIRAIFKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
LAKMASDYQKPHGLTVILPEQAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLEVPEVTLIDRFGRLGYDLYRKAR
GIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKELTILSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQ
DASQISQIALQLYEELSEKERGVRLLGITLTGF

Sequences:

>Translated_353_residues
MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSDPRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCP
QAVFISGNYEKYKAVGLQIRAIFKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
LAKMASDYQKPHGLTVILPEQAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLEVPEVTLIDRFGRLGYDLYRKAR
GIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKELTILSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQ
DASQISQIALQLYEELSEKERGVRLLGITLTGF
>Mature_353_residues
MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSDPRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCP
QAVFISGNYEKYKAVGLQIRAIFKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
LAKMASDYQKPHGLTVILPEQAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLEVPEVTLIDRFGRLGYDLYRKAR
GIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKELTILSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQ
DASQISQIALQLYEELSEKERGVRLLGITLTGF

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI84043967, Length=338, Percent_Identity=28.9940828402367, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI7706681, Length=339, Percent_Identity=28.9085545722714, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI154350220, Length=322, Percent_Identity=27.3291925465839, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI7705344, Length=115, Percent_Identity=44.3478260869565, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI5729982, Length=396, Percent_Identity=26.5151515151515, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786425, Length=348, Percent_Identity=40.2298850574713, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1787432, Length=212, Percent_Identity=29.2452830188679, Blast_Score=88, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI193205700, Length=419, Percent_Identity=30.0715990453461, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17537959, Length=370, Percent_Identity=26.4864864864865, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI193205702, Length=349, Percent_Identity=27.2206303724928, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI115534089, Length=125, Percent_Identity=39.2, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19923006, Length=413, Percent_Identity=24.6973365617433, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI21355641, Length=407, Percent_Identity=27.027027027027, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24644984, Length=407, Percent_Identity=27.027027027027, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24668444, Length=127, Percent_Identity=34.6456692913386, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39876; Mature: 39876

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSDPRQTGGRGVVSTCSYE
CEEEHHHHHHHHHEEEEEHHHEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCHHCCCH
ARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAIFKRYTDLIEPMSIDEAYL
HHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
DVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKFLAKMASDYQKPHGLTVILPE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEHH
QAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLEVPEVTLIDRFGRLGYDLYRKAR
HHHHHHHHHCHHHHHCCCHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHC
GIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKELTILSEKVALNLHQQEKAGKIVILK
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IRYEDFSTLTKRKSLAQKTQDASQISQIALQLYEELSEKERGVRLLGITLTGF
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECC
>Mature Secondary Structure
MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSDPRQTGGRGVVSTCSYE
CEEEHHHHHHHHHEEEEEHHHEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCHHCCCH
ARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAIFKRYTDLIEPMSIDEAYL
HHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
DVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKFLAKMASDYQKPHGLTVILPE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEHH
QAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLEVPEVTLIDRFGRLGYDLYRKAR
HHHHHHHHHCHHHHHCCCHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHC
GIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKELTILSEKVALNLHQQEKAGKIVILK
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IRYEDFSTLTKRKSLAQKTQDASQISQIALQLYEELSEKERGVRLLGITLTGF
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA