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The map label for this gene is ponA [H]

Identifier: 225860399

GI number: 225860399

Start: 380476

End: 382635

Strand: Reverse

Name: ponA [H]

Synonym: SPT_0412

Alternate gene names: 225860399

Gene position: 382635-380476 (Counterclockwise)

Preceding gene: 225860400

Following gene: 225860396

Centisome position: 18.12

GC content: 41.39

Gene sequence:

>2160_bases
ATGAACAAACAAACTATCCTGCGAATAGCTAAGTATGTGAGTATCTGCTTCTTAACTTTATTTATCACAGCAGTTATGCT
GGGAGGAGGCATATTTCTCTATTACGTCAGCAAAGCCCCAGCCCTATCTGAGAGCAAATTAGTCGCAACCACGTCTAGTA
AGATTTTCGATAGTAAAAACGAACTGATTGCCGATCTGGGCTCTGAACGCCGAGTAAACGCTCAGGCCAACGAAATTCCT
ACTGATTTGGTCAAGGCTATCGTATCGATCGAAGACCATCGTTTTTTCGATCATCGCGGTGTCGATACCATCCGAATTAT
AGGAGCTTTTTTACGTAACTTACAAAACAACTCCCTCCAGGGAGGATCTACCCTTACCCAACAATTAATTAAGTTGACCT
ATTTCTCAACTTCCACTTCTGATCAGACCATTTCACGTAAAGCCCAGGAAGCTTGGTTGGCTGTTCAATTAGAACAAAAA
GCCACAAAACAAGAAATCCTGACCTACTATGTGAATAAAGTTTACATGTCTAACGGAAACTATGGAATGCAGACGGCTGC
CCAAAACTATTACGGCAAAGATTTGCGAGAACTATCATTACCTCAACTTGCCCTACTAGCAGGAATGCCACAGGCTCCGA
ATCAATACGATCCCTATTCTCATCCAGAAGCTGCCCTTGACCGTCGTAACTTGGTACTTTCAGAGATGAAAGGTCAAGGC
TACATTTCTGCCGAACAGTATGAGAAGGCTATTAATACCCCTATTACTGATGGACTCCAAAGTTTGAAATCGGTCAATAG
CTATCCAGCATATATGGACAATTATCTCAAAGAGGTTATCGACCAAGTAGAACAAGAAACTGGCTATAACCTTCTAACTA
CTGGGATGGATGTTTACACAAATGTAGACCAAGAAGCTCAAAAACATCTGTGGGATATCTACAACTCCGATCAATACGTC
TCTTACCCTGACGATGATTTGCAAGTCGCATCTACGGTCGTAGATGTTTCAAATGGTAAAGTCATCGCACAACTTGGTGC
TCGTCATCAAGCAAGTAATGTTTCATTCGGTACCAACCAGGCCGTAGAAACCAATCGTGACTGGGGATCATCAATGAAAC
CAATCACTGACTATGCTCCCGCTTTAGAATATGGAGTCTATGACTCTACTGCTTCTATTGTACATGATGTCCCTTATAAC
TATCCTGGCACTGATACTCCACTCTACAACTGGGATCATGTCTACTTTGGAAACATTACAATCCAGTATGCTCTTCAACA
ATCACGAAATGTCACAGCCGTTGAGACTTTGAATAAGGTCGGTCTAGATAGAGCTAAAACCTTCCTTAATGGTCTTGGTA
TCGACTATCCAAGCATGCATTATGCAAACGCCATTTCAAGTAACACAACTGAATCCAACAAAAAATATGGTGCAAGTAGT
GAAAAAATGGCTGCTGCCTACGCTGCTTTTGCTAATGGTGGTATTTATCACAAACCAATGTATATCAATAAAATCGTCTT
TAGTGATGGTAGCGAAAAAGAATTTTCTGATGCTGGTACACGAGCTATGAAAGAGACTACTGCCTATATGATGACTGAAA
TGATGAAAACTGTTTTAACTTACGGAACAGGACGTGGAGCCTACCTACCATGGCTTCCACAAGCAGGTAAGACAGGTACT
TCTAACTATACTGACGAAGAAATTGAAAAGTATATCAAGAACACTGGCTACGTAGCTCCAGATGAAATGTTTGTGGGTTA
TACTCGTAAGTATTCTATGGCTGTATGGACAGGTTATTCGAATCGTTTAACTCCTATCGTTGGAGATGGTTTCCTAGTTG
CAGCTAAAGTTTATCGCTCTATGATGACCTACCTGTCTGAAGGAAGCAATCCAGAGGATTGGAATATACCAGAGGGGCTC
TACAGAAATGGAGAATTCGTATTTAAAAATGGTGCTCGTTCTACGTGGAGCTCACCTGCTCCACAACAACCCCCATCAAC
TGAAAGTTCAAGCTCATCATCAGATAGTTCAACTTCACAGTCTAGCTCAACCACTCCAAGCACAAATAATAGTACGACTA
CCAATCCTAACAATAATACGCAACAATCAAATACAACCCCTGATCAACAAAATCAGAATCCTCAACCAGCACAACCATAA

Upstream 100 bases:

>100_bases
GATATATTCGAGAATATGGATATGAAATCAAGGCTGGTGCCTTCCCTCAAATTCCCTATCTCAATGTTATCAAAGAACAT
TTATTAGGTGGTAAAACAAG

Downstream 100 bases:

>100_bases
CATTTATCACCCAGATTTTCTGGGTGATTTTGTCATAGCACTATTGTATTTATGTGCTTAACAAAAGTCTGAGAAGACAT
CTTCTCAGATTTTTTCTGTA

Product: penicillin-binding protein 1a

Products: NA

Alternate protein names: PBP-1A [H]

Number of amino acids: Translated: 719; Mature: 719

Protein sequence:

>719_residues
MNKQTILRIAKYVSICFLTLFITAVMLGGGIFLYYVSKAPALSESKLVATTSSKIFDSKNELIADLGSERRVNAQANEIP
TDLVKAIVSIEDHRFFDHRGVDTIRIIGAFLRNLQNNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAVQLEQK
ATKQEILTYYVNKVYMSNGNYGMQTAAQNYYGKDLRELSLPQLALLAGMPQAPNQYDPYSHPEAALDRRNLVLSEMKGQG
YISAEQYEKAINTPITDGLQSLKSVNSYPAYMDNYLKEVIDQVEQETGYNLLTTGMDVYTNVDQEAQKHLWDIYNSDQYV
SYPDDDLQVASTVVDVSNGKVIAQLGARHQASNVSFGTNQAVETNRDWGSSMKPITDYAPALEYGVYDSTASIVHDVPYN
YPGTDTPLYNWDHVYFGNITIQYALQQSRNVTAVETLNKVGLDRAKTFLNGLGIDYPSMHYANAISSNTTESNKKYGASS
EKMAAAYAAFANGGIYHKPMYINKIVFSDGSEKEFSDAGTRAMKETTAYMMTEMMKTVLTYGTGRGAYLPWLPQAGKTGT
SNYTDEEIEKYIKNTGYVAPDEMFVGYTRKYSMAVWTGYSNRLTPIVGDGFLVAAKVYRSMMTYLSEGSNPEDWNIPEGL
YRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSSSTTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP

Sequences:

>Translated_719_residues
MNKQTILRIAKYVSICFLTLFITAVMLGGGIFLYYVSKAPALSESKLVATTSSKIFDSKNELIADLGSERRVNAQANEIP
TDLVKAIVSIEDHRFFDHRGVDTIRIIGAFLRNLQNNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAVQLEQK
ATKQEILTYYVNKVYMSNGNYGMQTAAQNYYGKDLRELSLPQLALLAGMPQAPNQYDPYSHPEAALDRRNLVLSEMKGQG
YISAEQYEKAINTPITDGLQSLKSVNSYPAYMDNYLKEVIDQVEQETGYNLLTTGMDVYTNVDQEAQKHLWDIYNSDQYV
SYPDDDLQVASTVVDVSNGKVIAQLGARHQASNVSFGTNQAVETNRDWGSSMKPITDYAPALEYGVYDSTASIVHDVPYN
YPGTDTPLYNWDHVYFGNITIQYALQQSRNVTAVETLNKVGLDRAKTFLNGLGIDYPSMHYANAISSNTTESNKKYGASS
EKMAAAYAAFANGGIYHKPMYINKIVFSDGSEKEFSDAGTRAMKETTAYMMTEMMKTVLTYGTGRGAYLPWLPQAGKTGT
SNYTDEEIEKYIKNTGYVAPDEMFVGYTRKYSMAVWTGYSNRLTPIVGDGFLVAAKVYRSMMTYLSEGSNPEDWNIPEGL
YRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSSSTTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP
>Mature_719_residues
MNKQTILRIAKYVSICFLTLFITAVMLGGGIFLYYVSKAPALSESKLVATTSSKIFDSKNELIADLGSERRVNAQANEIP
TDLVKAIVSIEDHRFFDHRGVDTIRIIGAFLRNLQNNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAVQLEQK
ATKQEILTYYVNKVYMSNGNYGMQTAAQNYYGKDLRELSLPQLALLAGMPQAPNQYDPYSHPEAALDRRNLVLSEMKGQG
YISAEQYEKAINTPITDGLQSLKSVNSYPAYMDNYLKEVIDQVEQETGYNLLTTGMDVYTNVDQEAQKHLWDIYNSDQYV
SYPDDDLQVASTVVDVSNGKVIAQLGARHQASNVSFGTNQAVETNRDWGSSMKPITDYAPALEYGVYDSTASIVHDVPYN
YPGTDTPLYNWDHVYFGNITIQYALQQSRNVTAVETLNKVGLDRAKTFLNGLGIDYPSMHYANAISSNTTESNKKYGASS
EKMAAAYAAFANGGIYHKPMYINKIVFSDGSEKEFSDAGTRAMKETTAYMMTEMMKTVLTYGTGRGAYLPWLPQAGKTGT
SNYTDEEIEKYIKNTGYVAPDEMFVGYTRKYSMAVWTGYSNRLTPIVGDGFLVAAKVYRSMMTYLSEGSNPEDWNIPEGL
YRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSSSTTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP

Specific function: Cell wall formation [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=309, Percent_Identity=33.0097087378641, Blast_Score=175, Evalue=9e-45,
Organism=Escherichia coli, GI1786343, Length=521, Percent_Identity=30.7101727447217, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1788867, Length=565, Percent_Identity=27.787610619469, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1789601, Length=237, Percent_Identity=28.2700421940928, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 79750; Mature: 79750

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQTILRIAKYVSICFLTLFITAVMLGGGIFLYYVSKAPALSESKLVATTSSKIFDSKN
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCHHHHCCHH
ELIADLGSERRVNAQANEIPTDLVKAIVSIEDHRFFDHRGVDTIRIIGAFLRNLQNNSLQ
HHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCC
GGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAVQLEQKATKQEILTYYVNKVYMSNGN
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHEECCCC
YGMQTAAQNYYGKDLRELSLPQLALLAGMPQAPNQYDPYSHPEAALDRRNLVLSEMKGQG
CCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
YISAEQYEKAINTPITDGLQSLKSVNSYPAYMDNYLKEVIDQVEQETGYNLLTTGMDVYT
CCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHC
NVDQEAQKHLWDIYNSDQYVSYPDDDLQVASTVVDVSNGKVIAQLGARHQASNVSFGTNQ
CCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHEECCCCEEEEECCCCCCCCCCCCCCCC
AVETNRDWGSSMKPITDYAPALEYGVYDSTASIVHDVPYNYPGTDTPLYNWDHVYFGNIT
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEECCEE
IQYALQQSRNVTAVETLNKVGLDRAKTFLNGLGIDYPSMHYANAISSNTTESNKKYGASS
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCH
EKMAAAYAAFANGGIYHKPMYINKIVFSDGSEKEFSDAGTRAMKETTAYMMTEMMKTVLT
HHHHHHHHHHHCCCEEECCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGTGRGAYLPWLPQAGKTGTSNYTDEEIEKYIKNTGYVAPDEMFVGYTRKYSMAVWTGYS
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEEEECCC
NRLTPIVGDGFLVAAKVYRSMMTYLSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPA
CCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCCCC
PQQPPSTESSSSSSDSSTSQSSSTTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKQTILRIAKYVSICFLTLFITAVMLGGGIFLYYVSKAPALSESKLVATTSSKIFDSKN
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCHHHHCCHH
ELIADLGSERRVNAQANEIPTDLVKAIVSIEDHRFFDHRGVDTIRIIGAFLRNLQNNSLQ
HHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCC
GGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAVQLEQKATKQEILTYYVNKVYMSNGN
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHEECCCC
YGMQTAAQNYYGKDLRELSLPQLALLAGMPQAPNQYDPYSHPEAALDRRNLVLSEMKGQG
CCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
YISAEQYEKAINTPITDGLQSLKSVNSYPAYMDNYLKEVIDQVEQETGYNLLTTGMDVYT
CCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHC
NVDQEAQKHLWDIYNSDQYVSYPDDDLQVASTVVDVSNGKVIAQLGARHQASNVSFGTNQ
CCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHEECCCCEEEEECCCCCCCCCCCCCCCC
AVETNRDWGSSMKPITDYAPALEYGVYDSTASIVHDVPYNYPGTDTPLYNWDHVYFGNIT
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEECCEE
IQYALQQSRNVTAVETLNKVGLDRAKTFLNGLGIDYPSMHYANAISSNTTESNKKYGASS
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCH
EKMAAAYAAFANGGIYHKPMYINKIVFSDGSEKEFSDAGTRAMKETTAYMMTEMMKTVLT
HHHHHHHHHHHCCCEEECCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGTGRGAYLPWLPQAGKTGTSNYTDEEIEKYIKNTGYVAPDEMFVGYTRKYSMAVWTGYS
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEEEECCC
NRLTPIVGDGFLVAAKVYRSMMTYLSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPA
CCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCCCC
PQQPPSTESSSSSSDSSTSQSSSTTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1396576; 11463916 [H]