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The map label for this gene is int [H]

Identifier: 225860217

GI number: 225860217

Start: 198096

End: 199262

Strand: Reverse

Name: int [H]

Synonym: SPT_0222

Alternate gene names: 225860217

Gene position: 199262-198096 (Counterclockwise)

Preceding gene: 225860218

Following gene: 225860216

Centisome position: 9.43

GC content: 37.87

Gene sequence:

>1167_bases
ATGAAAATAACTGAAGTTATAAAAAAAGACGGTAGCAAAGTTTATCGTGCTAATGTATATCTAGGAGTTGACCAGGTAAC
AGGAAAGAAAGTAAAAACCAAAGTAACAGGTCGGACACAAAAGGAAGTTAAGCAGAAAGCCAATCAAGAAAAAATAGCTT
TTCAAAAAGCAGGATCTACCAGACAAAAGGCTGTTACCATAAAAAACTATCAAGAATTGGCAACTCTCTGGTTGGAAAGT
TATAAGAATACGGTTAAGCCAAACACTCAAGGCAATGTTAAAAAGCTAATCGATAATCATATATTGCCTATTTTTGGGGC
TTACAAGCTCGATAAGCTCACTACTCCACTTATCCAGTCAATTATCAACGAACTTGCTGACAAGACTAATAGAGGGGAAA
AAGCGGCTTTTCTACATTATGACAAGATACACGCTTTAAACAAACGTATCTTACAGTATGGCGTAACCATGCAGGCTATA
CCGTCCAATCCTGCGCGTGATGTTGTTTTACCTCGTAACACTCAGAAAGCAAAGCGGAAAAAAGTAAAGCACTTTGAAAA
TCAAGAACTAAAAAAATTTCTTGGTTATCTCGATAATTTAGATACTCATAGATATCGTTACTACTATGAGACAACACTCT
ATAAGTTCCTATTGGCTACTGGTTGCCGTATCAATGAAGCTTTAGCTCTTTCCTGGTCTGATATTGACTTAGATAATGCC
GTTGTGCATATTACCAAAACACTAAATCGGGATATAGAAATCAATAGCCCAAAGTCTAAGGCTAGTTATCGGGATATAGA
TATAGATCAAGCGACTGTTAGCATGCTGAAGCAGTACAAACTACGCCAAACTAAAGAGGCCTGGAAGATAGGGCAACGTG
AACGCGTGGTATTTTCTGATTTCATTCATGAGTATCCTAGCAGTTCAAGACTTAAAAGAAGATTGCAAACACATTTTAAG
CGTGCTGATGTTCCTAACATTGGATTTCACGGATTCCGTCACACTCACGCTAGTCTCTTGCTTAACTCTGGAATACCATA
CAAGGAACTCCAGTACCGCCTAGGTCATTCCACTTTATCAATGACCATGGATATATATAGTCACCTCTCAAAAGAGAACG
CAAAAAAAGCAGTATCATTCTACGAAACTGCACTAAAAGCACTATAG

Upstream 100 bases:

>100_bases
AATAAAACGAAAATAAGGGGGTTCTCGTAGCTCCTAGCATGATATAAAACCTTTTTGATAATGGCTTGCCTGCTGATGTA
TTTTAGAAAGGTTTATCATC

Downstream 100 bases:

>100_bases
GGGGAGCAAAAAGGGGAGCAAATTTAAAAATCAACATTTCAAGACAAAGTAAAAATCCAATAACAACGGTGTCTTTGTAA
GATTTTATTTGAAAAAAGCC

Product: prophage Sa05, site-specific recombinase, phage integrase family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKITEVIKKDGSKVYRANVYLGVDQVTGKKVKTKVTGRTQKEVKQKANQEKIAFQKAGSTRQKAVTIKNYQELATLWLES
YKNTVKPNTQGNVKKLIDNHILPIFGAYKLDKLTTPLIQSIINELADKTNRGEKAAFLHYDKIHALNKRILQYGVTMQAI
PSNPARDVVLPRNTQKAKRKKVKHFENQELKKFLGYLDNLDTHRYRYYYETTLYKFLLATGCRINEALALSWSDIDLDNA
VVHITKTLNRDIEINSPKSKASYRDIDIDQATVSMLKQYKLRQTKEAWKIGQRERVVFSDFIHEYPSSSRLKRRLQTHFK
RADVPNIGFHGFRHTHASLLLNSGIPYKELQYRLGHSTLSMTMDIYSHLSKENAKKAVSFYETALKAL

Sequences:

>Translated_388_residues
MKITEVIKKDGSKVYRANVYLGVDQVTGKKVKTKVTGRTQKEVKQKANQEKIAFQKAGSTRQKAVTIKNYQELATLWLES
YKNTVKPNTQGNVKKLIDNHILPIFGAYKLDKLTTPLIQSIINELADKTNRGEKAAFLHYDKIHALNKRILQYGVTMQAI
PSNPARDVVLPRNTQKAKRKKVKHFENQELKKFLGYLDNLDTHRYRYYYETTLYKFLLATGCRINEALALSWSDIDLDNA
VVHITKTLNRDIEINSPKSKASYRDIDIDQATVSMLKQYKLRQTKEAWKIGQRERVVFSDFIHEYPSSSRLKRRLQTHFK
RADVPNIGFHGFRHTHASLLLNSGIPYKELQYRLGHSTLSMTMDIYSHLSKENAKKAVSFYETALKAL
>Mature_388_residues
MKITEVIKKDGSKVYRANVYLGVDQVTGKKVKTKVTGRTQKEVKQKANQEKIAFQKAGSTRQKAVTIKNYQELATLWLES
YKNTVKPNTQGNVKKLIDNHILPIFGAYKLDKLTTPLIQSIINELADKTNRGEKAAFLHYDKIHALNKRILQYGVTMQAI
PSNPARDVVLPRNTQKAKRKKVKHFENQELKKFLGYLDNLDTHRYRYYYETTLYKFLLATGCRINEALALSWSDIDLDNA
VVHITKTLNRDIEINSPKSKASYRDIDIDQATVSMLKQYKLRQTKEAWKIGQRERVVFSDFIHEYPSSSRLKRRLQTHFK
RADVPNIGFHGFRHTHASLLLNSGIPYKELQYRLGHSTLSMTMDIYSHLSKENAKKAVSFYETALKAL

Specific function: Putative integrase that is involved in the insertion of the integrative and conjugative element ICEBs1. Required for the excision of ICEBs1 from the donor cell genome and subsequent integration in the recipient cell genome. Appears not to be transferred t

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1787607, Length=336, Percent_Identity=25.297619047619, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 44810; Mature: 44810

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITEVIKKDGSKVYRANVYLGVDQVTGKKVKTKVTGRTQKEVKQKANQEKIAFQKAGST
CCHHHHHHCCCCEEEEEEEEEECHHHCCCCCEEEECCCHHHHHHHHCCHHHHHHHHCCCC
RQKAVTIKNYQELATLWLESYKNTVKPNTQGNVKKLIDNHILPIFGAYKLDKLTTPLIQS
HHHEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHCCHHHHHHHHHHHHH
IINELADKTNRGEKAAFLHYDKIHALNKRILQYGVTMQAIPSNPARDVVLPRNTQKAKRK
HHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCEEEECCCCCCCCEECCCCCHHHHHH
KVKHFENQELKKFLGYLDNLDTHRYRYYYETTLYKFLLATGCRINEALALSWSDIDLDNA
HHHHCCHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCH
VVHITKTLNRDIEINSPKSKASYRDIDIDQATVSMLKQYKLRQTKEAWKIGQRERVVFSD
HEEEEHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FIHEYPSSSRLKRRLQTHFKRADVPNIGFHGFRHTHASLLLNSGIPYKELQYRLGHSTLS
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHH
MTMDIYSHLSKENAKKAVSFYETALKAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKITEVIKKDGSKVYRANVYLGVDQVTGKKVKTKVTGRTQKEVKQKANQEKIAFQKAGST
CCHHHHHHCCCCEEEEEEEEEECHHHCCCCCEEEECCCHHHHHHHHCCHHHHHHHHCCCC
RQKAVTIKNYQELATLWLESYKNTVKPNTQGNVKKLIDNHILPIFGAYKLDKLTTPLIQS
HHHEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHCCHHHHHHHHHHHHH
IINELADKTNRGEKAAFLHYDKIHALNKRILQYGVTMQAIPSNPARDVVLPRNTQKAKRK
HHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCEEEECCCCCCCCEECCCCCHHHHHH
KVKHFENQELKKFLGYLDNLDTHRYRYYYETTLYKFLLATGCRINEALALSWSDIDLDNA
HHHHCCHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCH
VVHITKTLNRDIEINSPKSKASYRDIDIDQATVSMLKQYKLRQTKEAWKIGQRERVVFSD
HEEEEHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FIHEYPSSSRLKRRLQTHFKRADVPNIGFHGFRHTHASLLLNSGIPYKELQYRLGHSTLS
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHH
MTMDIYSHLSKENAKKAVSFYETALKAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]