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The map label for this gene is sacA [H]

Identifier: 225859555

GI number: 225859555

Start: 1715107

End: 1716426

Strand: Reverse

Name: sacA [H]

Synonym: SP70585_1846

Alternate gene names: 225859555

Gene position: 1716426-1715107 (Counterclockwise)

Preceding gene: 225859556

Following gene: 225859554

Centisome position: 78.57

GC content: 40.0

Gene sequence:

>1320_bases
ATGATAAATAAAACATTTACAGTAGAAAAAGCCAATCGTTTTATAGCAGAAAATAAACATCTCGTTAATACTCAATATAA
GCCTGAAGAACATTTTTCAGCTGAGATTGGTTGGATCAATGATCCAAATGGATTTGTTTATTTTCGTGGAGAATACCATC
TCTTTTATCAATTCTATCCATATGATAGTGTTTGGGGACCTATGCACTGGGGACATGCTAAAAGTAAGGACTTGGTGACT
TGGGAGCACTTGCCAGTGGCACTTGCTCCTGATCAAGATTATGACCGAAATGGTTGTTTCTCAGGCTCTGCCATTGTCAA
GGATGATCGCCTCTGGCTCATGTACACTGGGCATATCGAAGAAAAAACCGGTGTCCGCCAAGTGCAAAATATGGCATTTT
CAGACGACGGGATTCACTTTGAAAAGATTGAACAAAATCCAGTTGCAACTGGATCAGACTTGCCAGATGAGTTGATTGCT
GCTGATTTCCGTGATCCAAAACTCTTTGAAAAAGATGGACGCTATTACTCCGTAGTAGCTGCCAAACACAAGAATAATGT
GGGCTGTATCGTTCTACTAGGGTCCGATAACCTAGTAGAATGGCAGTTCGAATCCATCTTTTTAAAAGGGGGAGAACACC
AAGGCTTTATGTGGGAATGCCCAGATTACTTCGAGTTAGATGGGAAAGATTGCCTTATTATGTCACCCATGCGTTATCAG
CGTGAGGGAGACTCATATCATAACATCAACTCATCGCTTTTGTTCACGGGTAAGGTAGATTGGAGAGAAAAACGTTTTAT
CCCAGAATCAGTTCAAGAAATTGATCATGGCCAAGACTTCTATGCGCCTCAAACATTGTTGGACGATCAAAATCGTCGTA
TCCTGATTGCTTGGATGCAGACATGGGGGCGTACCCTTCCAACCCATGACCAAGAACACAAATGGGCATGTGCCATGACT
TTACCTAGAATTCTAAGATTGGAAGATGGCAAACTAAGACAATTCCCTGTTAAAAAAGGCCAATATCAAATCCAAATAGA
TAAAGATTGTCATTACCACTTAGGAAATGATACAGATTATCTTGAATTTGGTTATGACAGTAATGCGCAGCAAGTTTACA
TTGATCGTAGCCATCTTATTCAAAAAATTCTAGGTGAAGAAGAACAGGACACTAGTCGACGGTATGTAGATATTGAAGCT
AAAGAATTGGAAGTTGTTCTAGATAAAAATTCCATCGAGATTTTTGTCAATCAAGGTGAAGCAAGCTTGACTGCAACTTA
TTACTTAACGGTGCCAGCTGAGCTATCACGAATTGATTAA

Upstream 100 bases:

>100_bases
TATTTCAACGATTCCGTTGATTTTGGTCTATGTAGTCGCTCAGAAATATATTGTTCAAGGTCTGGGTGGTTCCGGAACTG
GAATAAAATAGGATATACTT

Downstream 100 bases:

>100_bases
AAATTAAGTTATTTCTCCTAAAGAAAAAGTTCTCTTTCTAAAATAGTGGAAAGAGGACTTTTTGTGTTTTGGGTATATAA
GCTTAGTTTATGGTATTTGT

Product: sucrose-6-phosphate hydrolase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNGFVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVT
WEHLPVALAPDQDYDRNGCFSGSAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIA
ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMWECPDYFELDGKDCLIMSPMRYQ
REGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDHGQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMT
LPRILRLEDGKLRQFPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTSRRYVDIEA
KELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID

Sequences:

>Translated_439_residues
MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNGFVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVT
WEHLPVALAPDQDYDRNGCFSGSAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIA
ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMWECPDYFELDGKDCLIMSPMRYQ
REGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDHGQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMT
LPRILRLEDGKLRQFPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTSRRYVDIEA
KELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID
>Mature_439_residues
MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNGFVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVT
WEHLPVALAPDQDYDRNGCFSGSAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIA
ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMWECPDYFELDGKDCLIMSPMRYQ
REGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDHGQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMT
LPRILRLEDGKLRQFPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTSRRYVDIEA
KELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID

Specific function: Unknown

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=509, Percent_Identity=25.147347740668, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 51257; Mature: 51257

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNGFVYFRGEYHLFYQFYP
CCCCEEEHHHHHHHHHCCCEEECCCCCCCHHCCEEECEEECCCCEEEEECCEEEEEEEEC
YDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSGSAIVKDDRLWLMYTGHIE
CCCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCCCCCCCCCCEEEECCCEEEEEECCCC
EKTGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIAADFRDPKLFEKDGRYYSVVA
CHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEE
AKHKNNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMWECPDYFELDGKDCLIMSPMRYQ
EECCCCCEEEEEECCCCEEEEEEEEEEEECCCCCCEEECCCCCEECCCCCEEEECCHHHH
REGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDHGQDFYAPQTLLDDQNRRILIAWMQ
CCCCCCCCCCCEEEEEECCCCHHHCCCHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEH
TWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQFPVKKGQYQIQIDKDCHYHLGNDTDY
HHCCCCCCCCCCCCEEEEECCCHHEEECCCCEEECCCCCCCEEEEECCCCEEECCCCCCE
LEFGYDSNAQQVYIDRSHLIQKILGEEEQDTSRRYVDIEAKELEVVLDKNSIEIFVNQGE
EEECCCCCCCEEEEEHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEEECCCEEEEEECCC
ASLTATYYLTVPAELSRID
CEEEEEEEEEECHHHCCCC
>Mature Secondary Structure
MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNGFVYFRGEYHLFYQFYP
CCCCEEEHHHHHHHHHCCCEEECCCCCCCHHCCEEECEEECCCCEEEEECCEEEEEEEEC
YDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSGSAIVKDDRLWLMYTGHIE
CCCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCCCCCCCCCCEEEECCCEEEEEECCCC
EKTGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIAADFRDPKLFEKDGRYYSVVA
CHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEE
AKHKNNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMWECPDYFELDGKDCLIMSPMRYQ
EECCCCCEEEEEECCCCEEEEEEEEEEEECCCCCCEEECCCCCEECCCCCEEEECCHHHH
REGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDHGQDFYAPQTLLDDQNRRILIAWMQ
CCCCCCCCCCCEEEEEECCCCHHHCCCHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEH
TWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQFPVKKGQYQIQIDKDCHYHLGNDTDY
HHCCCCCCCCCCCCEEEEECCCHHEEECCCCEEECCCCCCCEEEEECCCCEEECCCCCCE
LEFGYDSNAQQVYIDRSHLIQKILGEEEQDTSRRYVDIEAKELEVVLDKNSIEIFVNQGE
EEECCCCCCCEEEEEHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEEECCCEEEEEECCC
ASLTATYYLTVPAELSRID
CEEEEEEEEEECHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2254250; 1368686 [H]