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The map label for this gene is rfaI [H]

Identifier: 225859518

GI number: 225859518

Start: 1678156

End: 1679205

Strand: Reverse

Name: rfaI [H]

Synonym: SP70585_1807

Alternate gene names: 225859518

Gene position: 1679205-1678156 (Counterclockwise)

Preceding gene: 225859519

Following gene: 225859517

Centisome position: 76.86

GC content: 34.67

Gene sequence:

>1050_bases
ATGGGGGGCGACTTGATTGATTGCAAGTTGATTGGCTCACAGTATACAATGAACTGGTCTAATAAACTGCCTCATATCAA
TCATATGACCTTTGCACGCTATTTTATTCCAGATTTTGTAACAGAAGATAAAGTTCTCTATCTAGATAGTGATTTGATTG
TGACTGGTGATTTGACCGATTTGTTTGAATTAGACTTAGGTGAAAATTATTTGGCAGCAGCTCGTTCTTGCTTTGGAGCA
GGAGTCGGCTTCAATGCTGGTGTTCTCTTGATTAACAACAAAAAATGGGGATCTGAAACTATTCGACAAAAATTGATTGA
CTTAACAGAAAAAGAACATGAGAATGTGGAAGAAGGAGACCAGTCAATTTTGAATATGTTGTTTAAAGATCAATATAGTT
CCCTTGAAGATCAATATAATTTTCAAATAGGATATGATTATGGGGCGGCAGCCTTTAAACATCAATTCATTTTTGATATT
CCGCTCGAACCACTGCCACTAATTTTACACTATATTTCTCAGGATAAGCCTTGGAATCAATTTTCTGTTGGACGTCTAAG
AGAAGTTTGGTGGGAATACTCTTTGATGGATTGGTCTGTTATTTTAAATGAATGGTTTTCAAAGAGTGTGAAGTATCCTA
GTAAATCACAAATATTTAAGTTGCAATGTGTTAATTTAACGAATTCTTGGTGTGTCGAGAAAATCGATTATTTGGCGGAG
CAATTGCCAGAAGTTCATTTTCATATTGTTGCTTATACAAATATGGCAAATGAACTACTAGCTTTAACGCGTTTTCCTAA
TGTTACCGTATATCCAAATTCCTTACCAATGTTATTGGAACAAATAGTAATAGCTTCAGATTTGTATTTGGATTTGAATC
ATGATCGAAAATTAGAAGATGCATATGAGTTTGTGCTTAAGTACAAAAAACCAATGATAGCTTTCGACAATACTTGCTCT
GAAAATCTTTCTGAGATTTCATATGAAGGTATCTATCCAAGCTCCATTCCGAAAAAAATGGTTGCAGCAATCAGATCTTA
CATGAGGTAG

Upstream 100 bases:

>100_bases
GAAGTCACTCTGTAGACACAATAGTCATTTGAAAATTTATTTGCTCAATCAGGACATTCCCCAGGAATGGTTTAGTCAAA
TAAGAATATATTTACAAGAG

Downstream 100 bases:

>100_bases
AGAACAGTATGAGAAAATCAATAGTATTAGCGGCAGATAATGCCTATCTTATTCCTTTAGAGACGACTATAAAGTCTGTA
TTGTATCACAATAGAGATGT

Product: glycosyl transferase, family 8

Products: NA

Alternate protein names: Lipopolysaccharide 3-alpha-galactosyltransferase [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MGGDLIDCKLIGSQYTMNWSNKLPHINHMTFARYFIPDFVTEDKVLYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGA
GVGFNAGVLLINNKKWGSETIRQKLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDI
PLEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIFKLQCVNLTNSWCVEKIDYLAE
QLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPMLLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCS
ENLSEISYEGIYPSSIPKKMVAAIRSYMR

Sequences:

>Translated_349_residues
MGGDLIDCKLIGSQYTMNWSNKLPHINHMTFARYFIPDFVTEDKVLYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGA
GVGFNAGVLLINNKKWGSETIRQKLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDI
PLEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIFKLQCVNLTNSWCVEKIDYLAE
QLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPMLLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCS
ENLSEISYEGIYPSSIPKKMVAAIRSYMR
>Mature_348_residues
GGDLIDCKLIGSQYTMNWSNKLPHINHMTFARYFIPDFVTEDKVLYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAG
VGFNAGVLLINNKKWGSETIRQKLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIFKLQCVNLTNSWCVEKIDYLAEQ
LPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPMLLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSE
NLSEISYEGIYPSSIPKKMVAAIRSYMR

Specific function: Adds the galactose(I) group on the glucose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

Organism=Escherichia coli, GI1790057, Length=187, Percent_Identity=27.807486631016, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.44 [H]

Molecular weight: Translated: 40488; Mature: 40356

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGDLIDCKLIGSQYTMNWSNKLPHINHMTFARYFIPDFVTEDKVLYLDSDLIVTGDLTD
CCCCEEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCHH
LFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQKLIDLTEKEHENVEEGD
HHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHH
QSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIPLEPLPLILHYISQDKPWNQ
HHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCEEEECCCCHHHHHHHHHHCCCCCCC
FSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIFKLQCVNLTNSWCVEKIDYLAE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHH
QLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPMLLEQIVIASDLYLDLNHDRKLED
HCCCCEEEEEEEHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHEEEECCCCCCHHH
AYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSSIPKKMVAAIRSYMR
HHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
GGDLIDCKLIGSQYTMNWSNKLPHINHMTFARYFIPDFVTEDKVLYLDSDLIVTGDLTD
CCCEEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCHH
LFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQKLIDLTEKEHENVEEGD
HHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHH
QSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIPLEPLPLILHYISQDKPWNQ
HHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCEEEECCCCHHHHHHHHHHCCCCCCC
FSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIFKLQCVNLTNSWCVEKIDYLAE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHH
QLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPMLLEQIVIASDLYLDLNHDRKLED
HCCCCEEEEEEEHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHEEEECCCCCCHHH
AYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSSIPKKMVAAIRSYMR
HHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]