Click here to switch to the map view.

The map label for this gene is nanA [H]

Identifier: 225859431

GI number: 225859431

Start: 1587354

End: 1588271

Strand: Reverse

Name: nanA [H]

Synonym: SP70585_1716

Alternate gene names: 225859431

Gene position: 1588271-1587354 (Counterclockwise)

Preceding gene: 225859432

Following gene: 225859430

Centisome position: 72.7

GC content: 43.14

Gene sequence:

>918_bases
ATGTCAGATTTGAAAAAATACGAAGGTGTCATTCCAGCCTTCTACGCATGTTATGATGAGCAAGGAGAAGTAAGTCCAGA
GCGTACGCGTGCCTTGGTTCAATACTTCATTGATAAAGGTGTTCAAGGTCTTTATGTCAATGGTTCTTCTGGTGAATGTA
TCTACCAAAGCGTTGAAGATCGCAAGTTGATTTTGGAAGAAGTCATGGCGGTAGCCAAAGGTAAATTGACCATTATTGCC
CATGTTGCTTGCAATAATACTAAAGATAGTATGGAACTTGCTCGCCATGCTGAAAGCTTGGGAGTAGATGCTATTGCAAC
GATTCCACCAATTTACTTCCGCTTGCCAGAATACTCAGTTGCCAAATACTGGAACGATATTAGTTCTGCAGCTCCAAACA
CAGACTACGTGATTTACAACATTCCTCAATTGGCAGGGGTTGCTTTGACTCCAAGCCTTTACACAGAAATGTTGAAAAAT
CCTCGTGTTATCGGTGTTAAGAACTCTTCTATGCCAGTTCAAGATATCCAAACCTTTGTCAGCCTTGGCGGGGAAGACCA
TATTGTCTTTAACGGTCCAGATGAACAGTTCCTAGGTGGCCGCCTCATGGGTGCTAAGGCTGGTATCGGTGGTACTTATG
GTGCTATGCCAGAACTCTTCTTGAAACTCAATCAGTTGATTGCGGATAAGGACCTAGAAACAGCGCGTGAATTGCAGTAT
GCTATCAACGCAATCATTGGTAAACTCACTTCTGCTCATGGAAATATGTACGGTGTCATCAAAGAAGTCTTGAAAATCAA
TGAAGGCTTGAATATTGGATCTGTTCGTTCACCATTGACACCAGTAACTGAAGAAGATCGTCCAGTTGTAGAAGCGGCTG
CAGCCTTGATTCGTGAAACCAAGGAGCGCTTCCTCTAA

Upstream 100 bases:

>100_bases
AGTGTCTATAAGATATTGAAAGCCTTTTCGGTAAAGTGTATACTAAAGAAGTAAAGAAAAATCTGCTTTAGCAGAAAATA
AAAATAATAGGAGAAAATCT

Downstream 100 bases:

>100_bases
TCTAAAAGGAGGTATTTATGACATACTACGTTGCAATTGATATCGGTGGAACTAACATCAAGTATGGTTTGGTTGATCAA
GAGGGGCAACTTCTTGAATC

Product: N-acetylneuraminate lyase

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialic acid aldolase; Sialic acid lyase [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGLYVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIA
HVACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPELFLKLNQLIADKDLETARELQY
AINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVRSPLTPVTEEDRPVVEAAAALIRETKERFL

Sequences:

>Translated_305_residues
MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGLYVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIA
HVACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPELFLKLNQLIADKDLETARELQY
AINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVRSPLTPVTEEDRPVVEAAAALIRETKERFL
>Mature_304_residues
SDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGLYVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAH
VACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKNP
RVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPELFLKLNQLIADKDLETARELQYA
INAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVRSPLTPVTEEDRPVVEAAAALIRETKERFL

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate [H]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13540533, Length=302, Percent_Identity=29.8013245033113, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1789620, Length=293, Percent_Identity=32.4232081911263, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87082415, Length=240, Percent_Identity=27.0833333333333, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786463, Length=234, Percent_Identity=26.0683760683761, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788823, Length=289, Percent_Identity=22.1453287197232, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- InterPro:   IPR005264 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.1.3.3 [H]

Molecular weight: Translated: 33412; Mature: 33281

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGLYVNGSSGECIYQSVED
CCCHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
RKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSV
HHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCHHHHCCCCEEECCCHHH
AKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKNPRVIGVKNSSMPVQDIQTFV
HHHHHHHHHCCCCCCEEEEECHHHCCEEECHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
SLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPELFLKLNQLIADKDLETARELQY
HCCCCCEEEECCCCHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVRSPLTPVTEEDRPVVEAAAALIRET
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KERFL
HHHCC
>Mature Secondary Structure 
SDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGLYVNGSSGECIYQSVED
CCHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
RKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGVDAIATIPPIYFRLPEYSV
HHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCHHHHCCCCEEECCCHHH
AKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKNPRVIGVKNSSMPVQDIQTFV
HHHHHHHHHCCCCCCEEEEECHHHCCEEECHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
SLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPELFLKLNQLIADKDLETARELQY
HCCCCCEEEECCCCHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVRSPLTPVTEEDRPVVEAAAALIRET
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KERFL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA