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The map label for this gene is truA

Identifier: 225859353

GI number: 225859353

Start: 1514827

End: 1515576

Strand: Reverse

Name: truA

Synonym: SP70585_1638

Alternate gene names: 225859353

Gene position: 1515576-1514827 (Counterclockwise)

Preceding gene: 225859354

Following gene: 225859352

Centisome position: 69.37

GC content: 44.53

Gene sequence:

>750_bases
ATGACGAGATATAAAGCAACTATTTCCTATGATGGTTATGCCTTTGCTGGCTTTCAGCGCCAGCCTCATGCGCGTAGCGT
TCAGGAAGAAATTGAAAAAACCTTGACCAGATTAAATAAAGGGCAAGCCATTACTGTTCACGGTGCTGGTAGGACAGATA
GTGGGGTTCATGCCCTGGGACAGGTCATTCATTTTGACCTGCCTTATCAGATGGATGAGGAGAAACTCCGTTTTGCCTTG
GATACCCAGTCTCCTGAAGATATTGATGTGATTTCGATTGAGCTTGTGGCAGATGATTTTCATTGCCGTTATGCCAAGCA
TAGCAAGACCTATGAGTTTACTGTGGATAGAGGACGTCCCAAAAATCCGATGCGCCGTCACTATGCCACCCACTTTCCCT
ACCCACTCGATGTGGAACGAATGCAGATTGCAATCAAAAAGCTAGAGGGAACCCATGATTTTACCGGTTTTACAGCCTCT
GGGACTAGTGTAGAGGATAAGGTTCGCACCATCACAGAAGCTAGTTTAATAGTCGATGAGACAGGACAATTTTTGACCTT
TACCTTTTCCGGAAATGGTTTCTTGTATAAACAGATTCGCAATATGGTGGGGACTCTTCTCAAAATCGGTAACAACCGCA
TGCCAGTAGAGCAGATTGATCTCATCTTGGAGAAGAAGGACAGGCAACTTGCAGGTCCCACTGCAGCACCAAATGGTTTG
TATTTAAAGGAGATTCGTTATGAAGAATAA

Upstream 100 bases:

>100_bases
GTGGTTTGAAGAGATTTTCAAGAGTATAAGATTTTTATACCCATACAATATTTGTTTTAGGTCCTTGTTTACAGACTAAT
TTCTTGATAAAATAGAGGTT

Downstream 100 bases:

>100_bases
TCGTATTTTAGCACTTTCTGGAAATGATATTTTTAGTGGTGGTGGACTGTCAGCTGATTTGGCTACCTATACCTTGAACG
GCTTGCATGGGTTTGTAGCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAITVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFAL
DTQSPEDIDVISIELVADDFHCRYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGL
YLKEIRYEE

Sequences:

>Translated_249_residues
MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAITVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFAL
DTQSPEDIDVISIELVADDFHCRYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGL
YLKEIRYEE
>Mature_248_residues
TRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAITVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALD
TQSPEDIDVISIELVADDFHCRYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTASG
TSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGLY
LKEIRYEE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=276, Percent_Identity=31.8840579710145, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI199559785, Length=278, Percent_Identity=27.3381294964029, Blast_Score=78, Evalue=7e-15,
Organism=Escherichia coli, GI1788657, Length=242, Percent_Identity=35.9504132231405, Blast_Score=160, Evalue=8e-41,
Organism=Drosophila melanogaster, GI116007752, Length=311, Percent_Identity=27.0096463022508, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648455, Length=259, Percent_Identity=25.4826254826255, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281362146, Length=257, Percent_Identity=24.5136186770428, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28381; Mature: 28249

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAITVHGAGRTDSGVHALG
CCEEEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
QVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHCRYAKHSKTYEFTVDRGRP
HHHEECCCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCEEECCCCCEEEEEECCCCC
KNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTASGTSVEDKVRTITEASLIVDE
CCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHEEEEC
TGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGL
CCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCE
YLKEIRYEE
EEEEEECCC
>Mature Secondary Structure 
TRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAITVHGAGRTDSGVHALG
CEEEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
QVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHCRYAKHSKTYEFTVDRGRP
HHHEECCCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCEEECCCCCEEEEEECCCCC
KNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTASGTSVEDKVRTITEASLIVDE
CCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHEEEEC
TGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGL
CCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCE
YLKEIRYEE
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA