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The map label for this gene is glyS

Identifier: 225859236

GI number: 225859236

Start: 1395751

End: 1397787

Strand: Reverse

Name: glyS

Synonym: SP70585_1516

Alternate gene names: 225859236

Gene position: 1397787-1395751 (Counterclockwise)

Preceding gene: 225859237

Following gene: 225859235

Centisome position: 63.98

GC content: 41.68

Gene sequence:

>2037_bases
ATGACAAAAAACTTATTAGTAGAACTCGGTCTTGAAGAATTACCAGCCTATGTTGTCACACCAAGTGAAAAACAACTAGG
CGAAAAAATGGCAGCCTTCCTCAAGGGAAAACGCCTGTCTTTTGAAGCCATTCAAACCTTCTCAACACCACGTCGTTTGG
CTGTTCGTGTGACTGGTTTATCAGACAAACAGTCTGATTTGACAGAAGATTTCAAGGGACCAGCAAAGAAAATTGCCTTG
GATAGTGATGGAAACTTCACCAAAGCAGCTCAAGGATTTGTCCGTGGAAAAGGCTTGACTGTTGAAGATATCGAATTCCG
TGAAATCAAGGGTGAAGAATATGTCTATGTCACTAAGGAAGAAATTGGTCAAGCAGTTGAAGCCATTGTTCCAGGCATTG
TGGATGTATTGAAGTCATTGACTTTCCCTGTCAGCATGCACTGGGCGGGAAATAGCTTTGAATACATCCGCCCTGTTCAC
ACTTTAACTGTTCTCTTGGATGAGCAAGAGTTTGACTTGGATTTCCTTGATATCAAGGGAAGTCGTGTGAGCCGTGGCCA
TCGTTTCTTGGGGAAAGAAACCAAAATTCAGTCAGCATTGAGCTATGAAGAAGACCTTCGTAAGCAGTTTGTAATCGCAG
ATCCATGTGAACGTGAGCAAATGATTGTTGACCAAATCAAGGAAATTGAGGCAAAACATGATGTACGTATCGAAATTGAT
GCGGATTTGCTGAATGAAGTCTTGAATTTGGTTGAATACCCAACTGCCTTCATGGGAAGTTTTGATGCTAAATACCTTGA
AGTTCCAGAAGAAGTCTTGGTGACTTCTATGAAGGAACACCAGCGTTACTTTGTTGTTCGTGATCAAGATGGAAAACTCT
TGCCAAACTTCATTTCTGTTCGTAACGGAAACGCAGAGCGTTTGAAAAATGTCATTAAAGGAAATGAAAAAGTCTTGGTA
GCCCGCTTGGAAGACGGAGAATTCTTCTGGCGTGAAGACCAAAAATTGGTGATTTCAGATCTTGTTGAAAAATTAAACAA
TGTCACCTTCCATGAGAAGATTGGTTCCCTTCGTGAACACATGATTCGTACAGGTCAAATCACTGTACTTTTGGCAGAAA
AAGCTGATTTGTCAGTGGATGAAACAGTTGACCTTGCTCGTGCAGCAGCCATTTACAAGTTTGACTTATTGACAGGTATG
GTTGGTGAATTTGACGAACTCCAAGGAATTATGGGTGAAAAATACACCCTTCTTGCTGGTGAAACTCCAGCGGTGGCAGC
TGCTATTCGTGAACACTACATGCCTACATCAGCTGAAGGAGAACTTCCAGAGAGCAAGGTCGGCGCAGTTCTAGCCATTG
CAGACAAATTGGATACGATTTTGAGTTTCTTCTCAGTAGGATTGATTCCATCAGGTTCTAATGACCCTTATGCCCTTCGT
CGTGCAACTCAAGGTGTGGTTCGTATCTTGGATGCCTTTGGTTGGCACATTGCTATGGATGAGCTGATTGATAGCCTTTA
TGCATTGAAATTTGACAGTTTGACTTATGAAAATAAAGCAGAGGTTATGGACTTTATCAAGGCTCGTGTTGATAAGATGA
TGGGCTCTACTCCAAAAGATATCAAGGAAGCAGTTCTTGCAGGTTCAAACTTTGTTGTGGCAGATATGTTGGAAGCAGCA
AGTGCTCTCGTAGAAGTAAGCAAGGAAGAAGATTTTAAACCATCTGTTGAATCACTTTCTCGTGCCTTTAACCTGGCTGA
GAAGGCAGAAGGGGTTGCTACAGTTGATTCAGCACTATTTGAGAATGACCAAGAAAAAGCTTTGGCAGAAGCAGTAGAAA
CACTCGTTTTATCAGGACCTGCAAGTCAGCAATTGAAACAACTCTTTGCGCTTAGCCCAGTCATTGATGCTTTCTTTGAA
AATACTATGGTAATGGCTGAAGATCAGGCTGTCCGTCAAAATCGTTTGGCAATCTTGTCACAACTAACCAAGAAAGCAGC
TAAGTTTGCTTGTTTTAACCAAATTAACACTAAATAA

Upstream 100 bases:

>100_bases
AGCATTGGATTTCCATACAGACTCACAGAGTTAGTTAGCGACGTCCCCAGTACCTAAGGTGCATATCAAAAAAGATTGAA
GAAAATGTAAAGGAAAAAAC

Downstream 100 bases:

>100_bases
AATTTGATAAACGGACTTTATCTTATTACAAAGGAGAAGAAATGGATCCGAAAAAAATTGCTCGTATCAATGAGCTTGCT
AAAAAGAAAAAAACAGAAGG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAIQTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIAL
DSDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKEIEAKHDVRIEID
ADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLV
ARLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKADLSVDETVDLARAAAIYKFDLLTGM
VGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALR
RATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAA
SALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQLKQLFALSPVIDAFFE
NTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK

Sequences:

>Translated_678_residues
MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAIQTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIAL
DSDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKEIEAKHDVRIEID
ADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLV
ARLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKADLSVDETVDLARAAAIYKFDLLTGM
VGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALR
RATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAA
SALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQLKQLFALSPVIDAFFE
NTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK
>Mature_677_residues
TKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAIQTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALD
SDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVHT
LTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKEIEAKHDVRIEIDA
DLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVA
RLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKADLSVDETVDLARAAAIYKFDLLTGMV
GEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALRR
ATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAAS
ALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQLKQLFALSPVIDAFFEN
TMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=676, Percent_Identity=35.3550295857988, Blast_Score=357, Evalue=2e-99,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- InterPro:   IPR003607 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75528; Mature: 75397

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAIQTFSTPRRLAVRVTGL
CCCCCEEECCHHHCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEECC
SDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKE
CCCHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHCCCCCEEECCHHHCCCCCCEEEEEHH
EIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVHTLTVLLDEQEFDLDFLDIKG
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHEEEEECCCCCCCEEEECCC
SRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKEIEAKHDVRIEID
CHHHCCHHHHCCHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
ADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISV
HHHHHHHHHHHHCCHHHHCCCCCHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCCHHHHC
RNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREH
CCCCHHHHHHHHCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
MIRTGQITVLLAEKADLSVDETVDLARAAAIYKFDLLTGMVGEFDELQGIMGEKYTLLAG
HHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEC
ETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALR
CCHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
IKEAVLAGSNFVVADMLEAASALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALF
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
ENDQEKALAEAVETLVLSGPASQQLKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILS
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
QLTKKAAKFACFNQINTK
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAIQTFSTPRRLAVRVTGL
CCCCEEECCHHHCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEECC
SDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKE
CCCHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHCCCCCEEECCHHHCCCCCCEEEEEHH
EIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVHTLTVLLDEQEFDLDFLDIKG
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHEEEEECCCCCCCEEEECCC
SRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKEIEAKHDVRIEID
CHHHCCHHHHCCHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
ADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISV
HHHHHHHHHHHHCCHHHHCCCCCHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCCHHHHC
RNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREH
CCCCHHHHHHHHCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
MIRTGQITVLLAEKADLSVDETVDLARAAAIYKFDLLTGMVGEFDELQGIMGEKYTLLAG
HHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEC
ETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALR
CCHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
IKEAVLAGSNFVVADMLEAASALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALF
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
ENDQEKALAEAVETLVLSGPASQQLKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILS
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
QLTKKAAKFACFNQINTK
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA