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The map label for this gene is nadE

Identifier: 225859184

GI number: 225859184

Start: 1351905

End: 1352729

Strand: Reverse

Name: nadE

Synonym: SP70585_1458

Alternate gene names: 225859184

Gene position: 1352729-1351905 (Counterclockwise)

Preceding gene: 225859185

Following gene: 225859183

Centisome position: 61.92

GC content: 47.27

Gene sequence:

>825_bases
ATGAGTTTGCAAGAAACGATTATCCAAGAGCTGGGTGTCAAACCAGTGATTGATGCCCAGGAAGAAATCCGTCGTTCTAT
TGATTTCTTAAAAAGATATCTGAAAAAACATCCCTTCCTAAAAACCTTTGTACTAGGGATTTCTGGGGGACAAGACTCAA
CCTTGGCAGGGCGTTTGGCGCAATTAGCTATGGAAGAACTGCGAGCTGAAACGGGAGACGACAGCTACAAATTTATCGCT
GTCCGCCTGCCATACGGAGTGCAAGCTGATGAAGCAGATGCTCAAAAAGCCCTAGCCTTCATCCAGCCAGATGTCAGCTT
GGTTGTGAATATCAAGGAATCAGCTGATGCCATGACAGCTGCAGTTGAAGCGACAGGTAGTCCTGTTTCAGACTTCAACA
AGGGGAATATCAAGGCACGTTGCCGTATGATTGCTCAGTATGCCCTTGCTGGTTCCCATAGCGGAGCGGTCATTGGAACA
GACCACGCCGCGGAAAATATCACAGGCTTTTTTACCAAGTTTGGTGACGGTGGTGCGGATATTCTCCCTCTTTACCGCCT
CAATAAACGTCAAGGAAAACAACTCTTACAGGAACTTGGTGCAGACCCAGCCCTTTATGAAAAAATCCCAACGGCAGACC
TAGAAGAAGACAAACCAGGCCTAGCTGACGAAGTCGCACTTGGAGTCACCTATGCAGAGATTGACGATTACCTAGAAGGC
AAAACAATCAGCCCAGAAGCTCAAGCGACCATTGAAAACTGGTGGCACAAAGGCCAACACAAACGCCACTTACCCATCAC
CGTATTTGATGACTTTTGGGAGTAA

Upstream 100 bases:

>100_bases
CGCAGCACTATCCAGTGGATTTGGCGCGTGATGTATGGCAAGATAAGATGGACTTGATTGATAAGATGCGCAAGGAAGCC
CTTGGTGAAGGAGAAGAAGA

Downstream 100 bases:

>100_bases
AAACCTCCATTGGAGGTTTTTACCCTGAGCCTAGAAATGAGAAAGCGAAGAAGGTCCGGGGGACCTTTTTAGCTTCTTGC
CCTGAAATTAAAAAGCAAGA

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIA
VRLPYGVQADEADAQKALAFIQPDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQELGADPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEG
KTISPEAQATIENWWHKGQHKRHLPITVFDDFWE

Sequences:

>Translated_274_residues
MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIA
VRLPYGVQADEADAQKALAFIQPDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQELGADPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEG
KTISPEAQATIENWWHKGQHKRHLPITVFDDFWE
>Mature_273_residues
SLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAV
RLPYGVQADEADAQKALAFIQPDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGTD
HAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQELGADPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEGK
TISPEAQATIENWWHKGQHKRHLPITVFDDFWE

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=274, Percent_Identity=66.0583941605839, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30122; Mature: 29991

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLA
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCHHHHHHHH
QLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQPDVSLVVNIKESADAMTA
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHH
AVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGTDHAAENITGFFTKFGDGGAD
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCC
ILPLYRLNKRQGKQLLQELGADPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEG
EEHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHCC
KTISPEAQATIENWWHKGQHKRHLPITVFDDFWE
CCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCC
>Mature Secondary Structure 
SLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLA
CHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCHHHHHHHH
QLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQPDVSLVVNIKESADAMTA
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHH
AVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGTDHAAENITGFFTKFGDGGAD
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCC
ILPLYRLNKRQGKQLLQELGADPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEG
EEHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHCC
KTISPEAQATIENWWHKGQHKRHLPITVFDDFWE
CCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA