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The map label for this gene is pyrB

Identifier: 225859071

GI number: 225859071

Start: 1246284

End: 1247207

Strand: Reverse

Name: pyrB

Synonym: SP70585_1340

Alternate gene names: 225859071

Gene position: 1247207-1246284 (Counterclockwise)

Preceding gene: 225859072

Following gene: 225859070

Centisome position: 57.09

GC content: 43.07

Gene sequence:

>924_bases
ATGTCAGAAAATCAACAAGCATTGAACCATGTGGTGTCCATGGAAGACCTCACTGTCGATCAAGTAATGAAATTGATCAA
GCGAGGAATTGAGTTTAAAAATGGAGCCCAGCTTCCCTATGAAGACCATCCGATTGTTTCCAATCTCTTCTTTGAGGATT
CTACACGGACACATAAGTCCTTTGAAGTCGCAGAGATTAAACTTGGATTGGAACGACTTGACTTTGATGTGAAGACTAGT
TCGGTTAATAAGGGTGAGACACTTTATGACACCATTTTGACTCTGTCTGCTTTAGGAGTGGATGTCTGTGTGATTCGCCA
TCCAGAGGTCGACTACTATAGAGAATTGATTGCTAGTCCAACGATTACGACTTCCATCATCAATGGTGGAGACGGTTCGG
GTCAACACCCTAGCCAGAGCTTGCTTGATTTGATGACCATTTATGAGGAATTTGGCCACTTTGAGGGTCTTAAAGTTGCT
ATTGCAGGTGACTTGGACCACTCACGCGTTGCCAAATCCAATATGCAGATTTTGAAACGCTTGGGAGCTGAACTCTTTTT
CGCTGGACCTGAGGAATGGAGAAGTCAAGAGTTTGCAGACTATGGAAAGTTTGTAACCATTGATGAAATCATTGATCAGG
TTGATGTCATGATGTTTCTCCGTGTGCAACACGAACGCCATGATAGTGGAGCTGTATTTTCAAAAGAAGACTACCATGCC
CAACATGGCTTGACTCAAGAACGTTACGATCGTTTGAAAGAAACAGCAATCCTCATGCACCCAGCTCCAATCAATCGTGA
TGTAGAAATAGCAGACCACTTGGTTGAAGCACCAAAATCACGGATTGTCCAACAAATGACCAATGGTGTCTTTGTTCGAA
TGGCAATCTTAGAATCCGTACTAGCGAGTAGAAACGCCAACTAA

Upstream 100 bases:

>100_bases
AACCAGTCGTTCTGAAGAAATCATCGTAGAGATGACAGAACTTGATGACCAAGACAGAGTTCTGATTACTGAAGAAGCTT
AGAAAGTTAAAGGAGTAGCC

Downstream 100 bases:

>100_bases
AACACAAGACGTTAAAAGACACCAGTGAGCGTCCACGAGAGGTGAAAATATGACAAAAAGAATTCTAGTATTAGAAGATG
GCACAGTTTTTGAAGGCAAG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHPIVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTS
SVNKGETLYDTILTLSALGVDVCVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHA
QHGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILESVLASRNAN

Sequences:

>Translated_307_residues
MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHPIVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTS
SVNKGETLYDTILTLSALGVDVCVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHA
QHGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILESVLASRNAN
>Mature_306_residues
SENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHPIVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSS
VNKGETLYDTILTLSALGVDVCVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVAI
AGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHAQ
HGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILESVLASRNAN

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=310, Percent_Identity=33.2258064516129, Blast_Score=153, Evalue=2e-37,
Organism=Escherichia coli, GI2367364, Length=304, Percent_Identity=33.5526315789474, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI193204318, Length=326, Percent_Identity=32.8220858895706, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=273, Percent_Identity=35.1648351648352, Blast_Score=129, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24642586, Length=275, Percent_Identity=34.1818181818182, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34691; Mature: 34560

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHPIVSNLFFEDSTRTHKS
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCC
FEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDVCVIRHPEVDYYRELIASP
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHCCC
TITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVAIAGDLDHSRVAKSNMQILKR
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
LGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHA
HCHHEEECCHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
QHGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILESV
HHCCCHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHH
LASRNAN
HHHCCCC
>Mature Secondary Structure 
SENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHPIVSNLFFEDSTRTHKS
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCC
FEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDVCVIRHPEVDYYRELIASP
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHCCC
TITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVAIAGDLDHSRVAKSNMQILKR
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
LGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHA
HCHHEEECCHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
QHGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILESV
HHCCCHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHH
LASRNAN
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA