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The map label for this gene is carA

Identifier: 225859070

GI number: 225859070

Start: 1245155

End: 1246234

Strand: Reverse

Name: carA

Synonym: SP70585_1339

Alternate gene names: 225859070

Gene position: 1246234-1245155 (Counterclockwise)

Preceding gene: 225859071

Following gene: 225859069

Centisome position: 57.04

GC content: 43.89

Gene sequence:

>1080_bases
ATGACAAAAAGAATTCTAGTATTAGAAGATGGCACAGTTTTTGAAGGCAAGGCCTTCGGAGCAGATATTGATGTAACAGG
CGAAATCGTCTTTAATACAGGGATGACCGGCTACCAAGAATCCATTACAGACCAGTCTTATAATGGACAAATCTTGACCT
TTACTTATCCTTTGGTAGGAAATTATGGTATTAATCGTGATGATTATGAATCCATTATTCCAACTTGTAAGGGAGTCGTT
GTTTTTGAAGAAGCGCGTAGAGCTAGCAACTGGCGCAACCAAATGACCTTGGATGAATTTTTGAAAGCCAAGAAAATTCC
AGGGATTTCAGGAATTGATACGCGTGCTCTTACCAAGATTATCCGTAAGCATGGTACTATGCGTGCAACCTTGACCCATG
TTGGGGACAGTATGGACCATGTGACGGACCAGCTCCAAGCAACAGTTTTACCGACAGACAATATCAAACAGGTTTCTACT
AAAACGTCTTATCCAGCTCCAGGAGTTGGTTTGAGCGTGGTGCTAGTGGACTTTGGTCTCAAGCACTCAATCCTACGTGA
ACTTTCTAAACGCAACTGTAACGTGACAGTTGTTCCTTATTCGACAACGGCTGAAGAAATTCTCCATCTCAATCCTGATG
GAGTTATGTTGTCAAATGGTCCAGGTAACCCAGAAGACGTTCCACAAGCACTCGACATGATTCGCGGTGTACAAGGAAAA
ATTCCAATCTTTGGTATTTGTATGGGACACCAACTCTTTGCAATGGCAAACGGGGCTAAGACCTATAAGATGAAGTTTGG
CCACCGTGGATTCAACCATGCGGTACGTGAAATCGCAACAGGACGAGTAGACTTCACCAGCCAGAACCATGGTTATGCAG
TCAGCCGCGAGGATTTGCCAGAGCACTTGATTATTACCCACGAAGAAATCAATGACAAGTCAGTGGAAGGTGTGCGCCAC
AGATACCAACCTGCTTTCTCTGTTCAATACCACCCAGATGCAGCTCCTGGGCCACACGACGCTAGCTACCTATTTGACGA
GTTTATCGAGATGATGGAAGTTTTTAAACAATCAAACTAA

Upstream 100 bases:

>100_bases
GTTCGAATGGCAATCTTAGAATCCGTACTAGCGAGTAGAAACGCCAACTAAAACACAAGACGTTAAAAGACACCAGTGAG
CGTCCACGAGAGGTGAAAAT

Downstream 100 bases:

>100_bases
GAACGAGTAAAAGCTCGTCGGAGAATTTATGTAACTGAACACGGACGGAAAGCTCAGAATGTAGAGAAACCAACTTGGAT
TGTCAATCCTTCCTTGATTT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVV
VFEEARRASNWRNQMTLDEFLKAKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLSNGPGNPEDVPQALDMIRGVQGK
IPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVREIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRH
RYQPAFSVQYHPDAAPGPHDASYLFDEFIEMMEVFKQSN

Sequences:

>Translated_359_residues
MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVV
VFEEARRASNWRNQMTLDEFLKAKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLSNGPGNPEDVPQALDMIRGVQGK
IPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVREIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRH
RYQPAFSVQYHPDAAPGPHDASYLFDEFIEMMEVFKQSN
>Mature_358_residues
TKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVV
FEEARRASNWRNQMTLDEFLKAKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVSTK
TSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLSNGPGNPEDVPQALDMIRGVQGKI
PIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVREIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHR
YQPAFSVQYHPDAAPGPHDASYLFDEFIEMMEVFKQSN

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=362, Percent_Identity=40.3314917127072, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI169790915, Length=362, Percent_Identity=38.121546961326, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI21361331, Length=362, Percent_Identity=38.121546961326, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1786215, Length=376, Percent_Identity=43.8829787234042, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1789760, Length=140, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=369, Percent_Identity=40.9214092140921, Blast_Score=253, Evalue=9e-68,
Organism=Saccharomyces cerevisiae, GI6324878, Length=376, Percent_Identity=40.4255319148936, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=37.5634517766497, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322638, Length=147, Percent_Identity=34.0136054421769, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45555749, Length=375, Percent_Identity=42.4, Blast_Score=272, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24642586, Length=375, Percent_Identity=42.4, Blast_Score=272, Evalue=2e-73,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_STRPN (P63733)

Other databases:

- EMBL:   AE005672
- PIR:   C95148
- RefSeq:   NP_345740.1
- ProteinModelPortal:   P63733
- SMR:   P63733
- EnsemblBacteria:   EBSTRT00000025348
- GeneID:   931790
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_1276
- TIGR:   SP_1276
- GeneTree:   EBGT00050000027164
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   SPNE170187-1:SP_1276-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 39864; Mature: 39733

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 247-247 ACT_SITE 331-331 ACT_SITE 333-333

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLVG
CCCEEEEEECCCEEECCCCCCCCEEEEEEEEECCCCCHHHHHCCCCCCCEEEEEEECCCC
NYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLKAKKIPGISGIDTRALTKI
CCCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHH
IRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVSTKTSYPAPGVGLSVVLVDFGL
HHHCCCEEEEHHHHCCHHHHHHHHHEEEECCCCCCHHHHCCCCCCCCCCCCEEEEEHHCH
KHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLSNGPGNPEDVPQALDMIRGVQGK
HHHHHHHHHHCCCCEEEEECCCCHHHHEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCC
IPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVREIATGRVDFTSQNHGYAVSREDLP
CCEEEEECCCEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCEEECCCCCCEEECCCCCC
EHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDAAPGPHDASYLFDEFIEMMEVFKQSN
CEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLVG
CCEEEEEECCCEEECCCCCCCCEEEEEEEEECCCCCHHHHHCCCCCCCEEEEEEECCCC
NYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLKAKKIPGISGIDTRALTKI
CCCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHH
IRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVSTKTSYPAPGVGLSVVLVDFGL
HHHCCCEEEEHHHHCCHHHHHHHHHEEEECCCCCCHHHHCCCCCCCCCCCCEEEEEHHCH
KHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLSNGPGNPEDVPQALDMIRGVQGK
HHHHHHHHHHCCCCEEEEECCCCHHHHEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCC
IPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVREIATGRVDFTSQNHGYAVSREDLP
CCEEEEECCCEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCEEECCCCCCEEECCCCCC
EHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDAAPGPHDASYLFDEFIEMMEVFKQSN
CEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916