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The map label for this gene is topA

Identifier: 225859057

GI number: 225859057

Start: 1228631

End: 1230718

Strand: Reverse

Name: topA

Synonym: SP70585_1326

Alternate gene names: 225859057

Gene position: 1230718-1228631 (Counterclockwise)

Preceding gene: 225859058

Following gene: 225859056

Centisome position: 56.33

GC content: 42.72

Gene sequence:

>2088_bases
GTGGCTACGGCAACAAAAAAGAAAAAATCAACAGTTAAAAAAAATCTAGTCATCGTGGAGTCGCCTGCTAAGGCCAAGAC
GATTGAAAAATATCTAGGCAGAAACTACAAGGTTTTAGCCAGTGTTGGGCATATCCGTGATTTGAAGAAATCCAGTATGT
CCGTCGATATTGAAAATAATTATGAACCGCAATATATTAATATCCGAGGAAAAGGCCCTCTTATCAATGACTTGAAAAAA
GAAGCTAAAAAAGCTAATAAAGTTTTTCTCGCGAGTGACCCGGACCGTGAAGGAGAAGCGATTTCTTGGCATTTGGCCCA
TATTCTCAACTTGGATGAAAATGATGCCAACCGTGTGGTCTTCAATGAAATCACCAAGGATGCAGTCAAAAATGCTTTTA
AAGAACCTCGTAAGATCGATATGGACTTGGTCGATGCCCAACAAGCTCGTCGGATCTTGGACCGCTTGGTAGGGTATTCG
ATTTCGCCTATTTTGTGGAAGAAGGTCAAGAAGGGCTTGTCAGCAGGTCGCGTTCAGTCCATTGCCCTTAAACTCATCAT
TGACCGTGAAAATGAAATCAATGCCTTCCAGCCAGAAGAATACTGGACAGTTGATGCTGTCTTTAAAAAGGGAACCAAAC
AATTTCATGCTTCCTTCTATGGAGTAGATGGTAAAAAGATGAAACTGACCAGCAATGACGAAGTCAAGGAAGTCTTGTCT
CGTCTGACGAGTAAAGACTTTTCAGTAGATCAGGTGGATAAGAAAGAGCGCAAGCGCAATGCTCCTTTACCCTATACCAC
TTCATCTATGCAGATGGATGCTGCCAATAAAATCAATTTCCGTACTCGAAAAACCATGATGGTTGCCCAACAGCTCTATG
AAGGAATTAATATCGGTTCTGGTGTTCAAGGTTTGATTACCTATATGCGTACCGATTCGACTCGTATCAGTCCTGTAGCG
CAAAATGAGGCGGCAAGCTTCATTACGGATCGTTTTGGTAGTAAGTATTCTAAGCACGGTAGCAAGGTCAAAAACGCATC
AGGTGCTCAGGATGCCCATGAGGCTATTCGTCCGTCAAGTGTCTTTAATACACCAGAAAGCATCGCTAAGTATCTGGACA
AGGATCAGCTCAAGCTATATACCCTTATCTGGAATCGTTTTGTGGCTAGCCAGATGACAGCGGCCGTTTTTGATACCATG
GCTGTTAAATTGTCTCAAAAAGGGGTTCAATTTGCTGCCAATGGTAGTCAGGTTAAGTTTGATGGTTATCTTGCCATTTA
TAATGATTCTGACAAGAATAAGATGTTACCGGACATGGTTGTTGGAAATGTGGTCAAACAGGTCAATAGCAAACCAGAGC
AACATTTCACCCAACCGCCTGCCCGTTATTCTGAAGCAACACTGATTAAAACCTTGGAGGAAAATGGGGTTGGACGTCCA
TCAACCTACGCGCCAACCATTGAAACCATTCAGAAACGTTATTATGTTCGCCTGGCAGCCAAACGTTTTGAACCGACAGA
GTTGGGAGAAATTGTCAATAAGCTCATCGTTGAATATTTCCCAGATATCGTAAACGTGACCTTCACAGCTGAAATGGAAG
GTAAACTGGATGATGTCGAAGTTGGAAAAGAGCAGTGGCGACGGGTCATTGATGCCTTTTACAAACCATTCTCTAAAGAA
GTTGCCAAGGCTGAAGAAGAAATGGAAAAAATCCAGATTAAGGATGAACCAGCTGGATTTGACTGTGAAGTGTGTGGCAG
TCCAATGGTCATTAAACTTGGTCGTTTTGGTAAATTCTACGCTTGTAGCAATTTCCCAGATTGCCGTCATACCCAAGCAA
TCGTGAAAGAGATTGGTGTTGAGTGTCCAAGCTGTCATCAGGGACAAATTATTGAGCGAAAAACCAAGCGTAATCGCCTA
TTCTATGGTTGCAATCGCTATCCAGAATGTGAATTTACCTCTTGGGACAAGCCTGTTGGTCGTGACTGTCCAAAATGTGG
CAACTTCCTCATGGAGAAAAAAGTCCGTGGTGGTGGCAAGCAGGTTGTTTGTAGCAAAGGCGACTACGAGGAAGAAAAGA
TTAAATAA

Upstream 100 bases:

>100_bases
AGTATAAAACTTTTCTTCTATTATAGTAGCTAAGTCTTGACAGGGTTCAAAAAATGGTTTACACTTTATAAAGTTTATTA
CTTTGAAAAGGTGTGATACT

Downstream 100 bases:

>100_bases
GAGGAGGGGCCCGAAAGTAAATTTCAGGCTCCTTTTGGTTAGAACTTGACAAAATACATCATTTTATGCGAAACTAGAAG
AAGATTATTTTAACTAGGAG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGHIRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKK
EAKKANKVFLASDPDREGEAISWHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHASFYGVDGKKMKLTSNDEVKEVLS
RLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAANKINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVA
QNEAASFITDRFGSKYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQMTAAVFDTM
AVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGNVVKQVNSKPEQHFTQPPARYSEATLIKTLEENGVGRP
STYAPTIETIQKRYYVRLAAKRFEPTELGEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKE
VAKAEEEMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPSCHQGQIIERKTKRNRL
FYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQVVCSKGDYEEEKIK

Sequences:

>Translated_695_residues
MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGHIRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKK
EAKKANKVFLASDPDREGEAISWHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHASFYGVDGKKMKLTSNDEVKEVLS
RLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAANKINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVA
QNEAASFITDRFGSKYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQMTAAVFDTM
AVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGNVVKQVNSKPEQHFTQPPARYSEATLIKTLEENGVGRP
STYAPTIETIQKRYYVRLAAKRFEPTELGEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKE
VAKAEEEMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPSCHQGQIIERKTKRNRL
FYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQVVCSKGDYEEEKIK
>Mature_694_residues
ATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGHIRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKE
AKKANKVFLASDPDREGEAISWHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYSI
SPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHASFYGVDGKKMKLTSNDEVKEVLSR
LTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAANKINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQ
NEAASFITDRFGSKYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQMTAAVFDTMA
VKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGNVVKQVNSKPEQHFTQPPARYSEATLIKTLEENGVGRPS
TYAPTIETIQKRYYVRLAAKRFEPTELGEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEV
AKAEEEMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPSCHQGQIIERKTKRNRLF
YGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQVVCSKGDYEEEKIK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=617, Percent_Identity=26.0940032414911, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI4507635, Length=530, Percent_Identity=22.8301886792453, Blast_Score=106, Evalue=7e-23,
Organism=Escherichia coli, GI1787529, Length=745, Percent_Identity=38.3892617449664, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI48994931, Length=108, Percent_Identity=40.7407407407407, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1788061, Length=514, Percent_Identity=22.568093385214, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32563869, Length=649, Percent_Identity=25.4237288135593, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17555378, Length=452, Percent_Identity=25.4424778761062, Blast_Score=122, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=493, Percent_Identity=23.3265720081136, Blast_Score=113, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24585251, Length=632, Percent_Identity=22.9430379746835, Blast_Score=132, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640096, Length=505, Percent_Identity=22.5742574257426, Blast_Score=100, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 78683; Mature: 78552

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGHIRDLKKSSMSVDIENN
CCCCCHHHHHHHHHCEEEEECCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCEEEECCC
YEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAISWHLAHILNLDENDANRVV
CCCCEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEHHHCCCCCCCCCCHH
FNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYSISPILWKKVKKGLSAGRVQS
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHH
IALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHASFYGVDGKKMKLTSNDEVKEVLS
EEEEEEEECCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHH
RLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAANKINFRTRKTMMVAQQLYEGINIGS
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCC
GVQGLITYMRTDSTRISPVAQNEAASFITDRFGSKYSKHGSKVKNASGAQDAHEAIRPSS
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHCCCHH
VFNTPESIAKYLDKDQLKLYTLIWNRFVASQMTAAVFDTMAVKLSQKGVQFAANGSQVKF
HCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEE
DGYLAIYNDSDKNKMLPDMVVGNVVKQVNSKPEQHFTQPPARYSEATLIKTLEENGVGRP
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCC
STYAPTIETIQKRYYVRLAAKRFEPTELGEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVE
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHEEEEEEECCCCCCHHH
VGKEQWRRVIDAFYKPFSKEVAKAEEEMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFY
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEECCCCCCCCHHCCCCCEEEEECCCCCEE
ACSNFPDCRHTQAIVKEIGVECPSCHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVG
EECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCC
RDCPKCGNFLMEKKVRGGGKQVVCSKGDYEEEKIK
CCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHCCC
>Mature Secondary Structure 
ATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGHIRDLKKSSMSVDIENN
CCCCHHHHHHHHHCEEEEECCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCEEEECCC
YEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAISWHLAHILNLDENDANRVV
CCCCEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEHHHCCCCCCCCCCHH
FNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYSISPILWKKVKKGLSAGRVQS
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHH
IALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHASFYGVDGKKMKLTSNDEVKEVLS
EEEEEEEECCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHH
RLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAANKINFRTRKTMMVAQQLYEGINIGS
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCC
GVQGLITYMRTDSTRISPVAQNEAASFITDRFGSKYSKHGSKVKNASGAQDAHEAIRPSS
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHCCCHH
VFNTPESIAKYLDKDQLKLYTLIWNRFVASQMTAAVFDTMAVKLSQKGVQFAANGSQVKF
HCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEE
DGYLAIYNDSDKNKMLPDMVVGNVVKQVNSKPEQHFTQPPARYSEATLIKTLEENGVGRP
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCC
STYAPTIETIQKRYYVRLAAKRFEPTELGEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVE
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHEEEEEEECCCCCCHHH
VGKEQWRRVIDAFYKPFSKEVAKAEEEMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFY
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEECCCCCCCCHHCCCCCEEEEECCCCCEE
ACSNFPDCRHTQAIVKEIGVECPSCHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVG
EECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCC
RDCPKCGNFLMEKKVRGGGKQVVCSKGDYEEEKIK
CCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]