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The map label for this gene is truB

Identifier: 225858995

GI number: 225858995

Start: 1169240

End: 1170118

Strand: Reverse

Name: truB

Synonym: SP70585_1260

Alternate gene names: 225858995

Gene position: 1170118-1169240 (Counterclockwise)

Preceding gene: 225858996

Following gene: 225858994

Centisome position: 53.56

GC content: 45.16

Gene sequence:

>879_bases
ATGAACGGTATTATCAACTTAAAAAAAGAAGCAGGAATGACCTCGCATGATGCGGTTTTTAAACTGCGTAAGATTTTGGG
AACCAAGAAAATTGGTCATGGCGGAACCTTGGATCCGGATGTGGTGGGTGTTTTGCCGATTGCGGTTGGCAAGGCGACAC
GCATGGTCGAGTTTATGCAGGACGAGGGTAAGATCTATGAGGGGGAAATCACTCTGGGCTATTCCACGACGACTGAGGAT
GCTAGTGGGGAAGTGGTCGCAGAAACCCCTGTTTTGTCTCCCTTGGATGAAAAGCTTGTTGATGAAGCGATTGCTAGCTT
GACTGGGCCTATTACCCAGATTCCCCCTATGTATTCGGCAGTTAAGGTTAATGGTCGCAAGCTCTATGAGTATGCGCGTG
CTGGTCAGGAAGTGGAGCGTTCAGAACGTCAGGTGACCATTTATCAATTTGAGCGAACAAGTCCGATTTCTTATGATGGC
CAACTTGCCCGATTCACTTTTCGTGTAAAATGCAGTAAAGGGACTTACATCCGTACTTTGTCAGTTGATTTGGGTGAAAA
GCTTGGTTATGCGGCTCATATGTCCCATTTGACTCGTACTAGTGCTGCTGGCTTACAATTAGAAGATGCTCTTACCTTGG
AGGAAATTGCTGAAAAAGTAGAGGCTGGGCAATTAGATTTTCTCCATCCTTTAGAGATTGGGACAGGTGACCTTGTCAAA
GTTTTCCTAACTCCAGAAGATGCTACAGAAGTTCGCTTTGGTCGTTTTATTGAGCTAGACCAAACGGACAAAGAACTGGC
TGCCTTTGAAGATGATAAATTGTTAGCCATTCTAGAAAAACGGGGCAATCTCTATAAGCCAAGGAAGGTTTTTAGCTAG

Upstream 100 bases:

>100_bases
TAACAACAAATTGGAAGATGTCTCTGTTAAAAAATTGACCCGGAAAAATCCAACAATGAAAGCGAAGTTCGAAGCACTGA
AGGGGGAGTAGAAAGCAAAA

Downstream 100 bases:

>100_bases
ATCGTTTAGGAATAAAAATCGGGTGATAGATAACAATTGCTTGATAAAACCCCATACTAATAGTAGAATGGTTTTGGGAA
TTATAATATTCCAATTGTTG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGVLPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTED
ASGEVVAETPVLSPLDEKLVDEAIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERSERQVTIYQFERTSPISYDG
QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAEKVEAGQLDFLHPLEIGTGDLVK
VFLTPEDATEVRFGRFIELDQTDKELAAFEDDKLLAILEKRGNLYKPRKVFS

Sequences:

>Translated_292_residues
MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGVLPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTED
ASGEVVAETPVLSPLDEKLVDEAIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERSERQVTIYQFERTSPISYDG
QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAEKVEAGQLDFLHPLEIGTGDLVK
VFLTPEDATEVRFGRFIELDQTDKELAAFEDDKLLAILEKRGNLYKPRKVFS
>Mature_292_residues
MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGVLPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTED
ASGEVVAETPVLSPLDEKLVDEAIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERSERQVTIYQFERTSPISYDG
QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAEKVEAGQLDFLHPLEIGTGDLVK
VFLTPEDATEVRFGRFIELDQTDKELAAFEDDKLLAILEKRGNLYKPRKVFS

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=264, Percent_Identity=34.0909090909091, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI215599015, Length=210, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4503337, Length=210, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI2367200, Length=248, Percent_Identity=39.5161290322581, Blast_Score=170, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17553978, Length=240, Percent_Identity=28.75, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=218, Percent_Identity=33.4862385321101, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324037, Length=193, Percent_Identity=34.1968911917098, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI281364189, Length=291, Percent_Identity=28.8659793814433, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364187, Length=291, Percent_Identity=28.8659793814433, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364185, Length=291, Percent_Identity=28.8659793814433, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364183, Length=291, Percent_Identity=28.8659793814433, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI62471759, Length=291, Percent_Identity=28.8659793814433, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17975520, Length=291, Percent_Identity=28.8659793814433, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015225
- InterPro:   IPR014780 [H]

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32248; Mature: 32248

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGVLPIAVGKATRMVEFMQ
CCCCEECHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEHHHHCHHHHHHHHHH
DEGKIYEGEITLGYSTTTEDASGEVVAETPVLSPLDEKLVDEAIASLTGPITQIPPMYSA
CCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCE
VKVNGRKLYEYARAGQEVERSERQVTIYQFERTSPISYDGQLARFTFRVKCSKGTYIRTL
EEECCHHHHHHHHCCHHHHHCCCEEEEEEEECCCCCCCCCCEEEEEEEEEECCCCEEEEE
SVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAEKVEAGQLDFLHPLEIGTGDLVK
EECHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
VFLTPEDATEVRFGRFIELDQTDKELAAFEDDKLLAILEKRGNLYKPRKVFS
EEECCCCCCHHCCCEEEECCCCCHHHHHCCCCCEEEEEHHCCCCCCCHHCCC
>Mature Secondary Structure
MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGVLPIAVGKATRMVEFMQ
CCCCEECHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEHHHHCHHHHHHHHHH
DEGKIYEGEITLGYSTTTEDASGEVVAETPVLSPLDEKLVDEAIASLTGPITQIPPMYSA
CCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCE
VKVNGRKLYEYARAGQEVERSERQVTIYQFERTSPISYDGQLARFTFRVKCSKGTYIRTL
EEECCHHHHHHHHCCHHHHHCCCEEEEEEEECCCCCCCCCCEEEEEEEEEECCCCEEEEE
SVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAEKVEAGQLDFLHPLEIGTGDLVK
EECHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
VFLTPEDATEVRFGRFIELDQTDKELAAFEDDKLLAILEKRGNLYKPRKVFS
EEECCCCCCHHCCCEEEECCCCCHHHHHCCCCCEEEEEHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA