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The map label for this gene is lacF [H]

Identifier: 225858973

GI number: 225858973

Start: 1152695

End: 1153012

Strand: Reverse

Name: lacF [H]

Synonym: SP70585_1238

Alternate gene names: 225858973

Gene position: 1153012-1152695 (Counterclockwise)

Preceding gene: 225858974

Following gene: 225858972

Centisome position: 52.78

GC content: 42.77

Gene sequence:

>318_bases
ATGAATAGAGAAGAAGTAACATTGTTAGGTTTTGAAATCGTAGCCTATGCTGGCGATGCTCGTTCAAAACTATTGGAAGC
CTTGAAGGCTGCTGAAGTTGGTGATTTTGAAAAAGCGGACGCTCTGGTAGAGGAAGCTGGTAGCTGTATTGCAGAGGCTC
ACCACGCGCAAACAAGTCTATTGACTAAGGAAGCTTCTGGTGAGGACTTAGCGTATAGTGTGACCATGATGCATGGCCAA
GACCACTTGATGACAACTATCTTGTTAAAGGATTTGATGCACCATTTGATCGAACTTTACAAGAGAGGAGTTAAATAA

Upstream 100 bases:

>100_bases
GTCTTGATTTGTATAAAAGTGAACGAGTTTATCTAGTTCTACATATCCAACGTTTATTGTCATAAAAATTTATTTAAAAC
TATATAAGGAGAATTCTATC

Downstream 100 bases:

>100_bases
TGAACAAACTAATTGCATTTATCGAGAAAGGAAAGCCTTTCTTTGAGAAACTATCTCGTAATATCTATCTTCGTGCTATT
CGTGATGGTTTCATTGCAGG

Product: lactose-specific phosphotransferase enzyme iia component (pts system lactose-specific eiia component) (eiia-lac) (eiii-lac)

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Lac; EIII-Lac; PTS system lactose-specific EIIA component [H]

Number of amino acids: Translated: 105; Mature: 105

Protein sequence:

>105_residues
MNREEVTLLGFEIVAYAGDARSKLLEALKAAEVGDFEKADALVEEAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQ
DHLMTTILLKDLMHHLIELYKRGVK

Sequences:

>Translated_105_residues
MNREEVTLLGFEIVAYAGDARSKLLEALKAAEVGDFEKADALVEEAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQ
DHLMTTILLKDLMHHLIELYKRGVK
>Mature_105_residues
MNREEVTLLGFEIVAYAGDARSKLLEALKAAEVGDFEKADALVEEAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQ
DHLMTTILLKDLMHHLIELYKRGVK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1447

COG function: function code G; Phosphotransferase system cellobiose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788031, Length=90, Percent_Identity=37.7777777777778, Blast_Score=65, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003188 [H]

Pfam domain/function: PF02255 PTS_IIA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 11541; Mature: 11541

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS51095 PTS_EIIA_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREEVTLLGFEIVAYAGDARSKLLEALKAAEVGDFEKADALVEEAGSCIAEAHHAQTSL
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKRGVK
HHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNREEVTLLGFEIVAYAGDARSKLLEALKAAEVGDFEKADALVEEAGSCIAEAHHAQTSL
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKRGVK
HHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2125052; 1901863; 9261069 [H]