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The map label for this gene is lacE [H]

Identifier: 225858972

GI number: 225858972

Start: 1151004

End: 1152695

Strand: Reverse

Name: lacE [H]

Synonym: SP70585_1237

Alternate gene names: 225858972

Gene position: 1152695-1151004 (Counterclockwise)

Preceding gene: 225858973

Following gene: 225858971

Centisome position: 52.76

GC content: 41.02

Gene sequence:

>1692_bases
ATGAACAAACTAATTGCATTTATCGAGAAAGGAAAGCCTTTCTTTGAGAAACTATCTCGTAATATCTATCTTCGTGCTAT
TCGTGATGGTTTCATTGCAGGTATGCCTGTTATTCTCTTCTCAAGTATCTTTATCTTGATTGCCTTTGTACCAAACTCAT
GGGGCTTTAAATGGTCTGATGAAGTTGTAGCCTTTCTGATGAAACCTTATAGCTATTCTATGGGTATTCTGGCTCTCTTG
GTAGCTGGTACAACAGCTAAGTCATTGACCGACTCAGTAAACCGTAGCATGGAGAAAACCAATCAAATCAACTATATGTC
AACGCTGTTAGCAGCGATTGTTGGTTTGTTGATGTTGGCAGCTGATCCTATCGAAAATGGTCTAGCTACTGGATTCTTGG
GGACAAAAGGTTTGCTTTCAGCCTTCCTTGCTGCCTTTGTTACTGTAGCCATCTATAAGGTTTGTGTTAAGAACAACGTC
ACTATTCGTATGCCTGACGAAGTTCCACCAAATATCTCACAAGTCTTTAAAGATGTGATTCCATTCACTCTATCTGTTGT
TTCTCTTTATGCTCTTGACTTATTAGCACGTCATTTTGTTGGTTCTAGTGTGGCAGAATCAATCGGTAAATTCTTCGCAC
CACTCTTCTCAGCAGCAGACGGATACCTTGGTATTACCATTATCTTTGGTGCCTTTGCCTTCTTCTGGTTTGTTGGGATT
CATGGTCCATCTATCGTTGAACCAGCTATCGCAGCTATTACCTATGCCAATGCCGAAGTCAACTTGAACCTTCTCCAACA
AGGTATGCACGCTGACAAGATTCTTACTTCTGGTACACAAATGTTTATCGTTACCATGGGTGGTACTGGTGCGACACTGG
TTGTTCCATTCATGTTCATGTGGTTAACAAAATCAAAACGTAACCGTGCAATCGGACGTGCTTCAGTAGTTCCTACCTTC
TTCGGTGTAAATGAGCCAATCTTGTTTGGTGCACCTCTTGTTTTGAATCCAATCTTCTTCATTCCATTTATCTTTGCTCC
AATTGCAAACGTATGGATTTTCAAATTCTTTATTGAAACTCTTGGAATGAACTCATTCACTGCTAATCTACCATGGACAA
CTCCAGCTCCACTAGGTCTAGTTCTTGGAACTAACTTCCAAGTGCTATCATTCATTCTTGCTGCCCTTCTAATCGTGGTT
GACGTTGTCATTTACTATCCATTCCTTAAGGTCTATGATGAACAAATTCTTGAAGAAGAACGTTCAGGTAAGTCTAATGA
TGAATTGAAAGAAAAAGTTGCTGCAAACTTCAACACTGCAAAAGCGGATGCTATTCTTGAAAAAGCGGGTGTCGATGCAG
CACAAAATACCATCACTGAAGAAACAAATGTCCTCGTTCTCTGTGCTGGTGGAGGTACAAGTGGACTCCTTGCAAATGCT
TTGAATAAGGCAGCAGCAGAATACAATGTCCCTGTGAAAGCAGCAGCAGGCGGCTATGGTGCTCACCGTGAAATGTTGCC
AGAGTTTGATTTGGTTATCCTTGCTCCTCAAGTTGCTTCAAACTTTGAAGATATGAAGGCAGAAACAGATAAGCTCGGTA
TTAAACTTGCTAAGACAGAAGGCGCTCAATACATCAAATTAACTCGTGATGGAAAAGGTGCCCTAGCATTCGTACAAGAG
CAATTCGATTAA

Upstream 100 bases:

>100_bases
GTGTGACCATGATGCATGGCCAAGACCACTTGATGACAACTATCTTGTTAAAGGATTTGATGCACCATTTGATCGAACTT
TACAAGAGAGGAGTTAAATA

Downstream 100 bases:

>100_bases
GGCTAGGAACTGTGAAACAGTCTCCCATCGTTACGGAAATCGCTATGGCGAATTTCCTAATCGCCTTATTAATTCGTCGG
TAAAAAGATATCGTTTTTAC

Product: PTS system, lactose-specific IIC component

Products: NA

Alternate protein names: EIICB-Lac; EII-Lac; Lactose permease IIC component; PTS system lactose-specific EIIC component; Lactose-specific phosphotransferase enzyme IIB component; PTS system lactose-specific EIIB component [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIFILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALL
VAGTTAKSLTDSVNRSMEKTNQINYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSAADGYLGITIIFGAFAFFWFVGI
HGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILTSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTF
FGVNEPILFGAPLVLNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFILAALLIVV
DVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLANA
LNKAAAEYNVPVKAAAGGYGAHREMLPEFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQE
QFD

Sequences:

>Translated_563_residues
MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIFILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALL
VAGTTAKSLTDSVNRSMEKTNQINYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSAADGYLGITIIFGAFAFFWFVGI
HGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILTSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTF
FGVNEPILFGAPLVLNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFILAALLIVV
DVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLANA
LNKAAAEYNVPVKAAAGGYGAHREMLPEFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQE
QFD
>Mature_563_residues
MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIFILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALL
VAGTTAKSLTDSVNRSMEKTNQINYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSAADGYLGITIIFGAFAFFWFVGI
HGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILTSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTF
FGVNEPILFGAPLVLNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFILAALLIVV
DVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLANA
LNKAAAEYNVPVKAAAGGYGAHREMLPEFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQE
QFD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=460, Percent_Identity=25.6521739130435, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014350
- InterPro:   IPR003501
- InterPro:   IPR013012
- InterPro:   IPR003352
- InterPro:   IPR004501
- InterPro:   IPR004801 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 61079; Mature: 61079

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS51100 PTS_EIIB_TYPE_3 ; PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIFILIAFVPNSWGFKWSD
CCHHHHHHHCCCHHHHHHCCCEEEEEHHCCHHCCCHHHHHHHHHHHHHCCCCCCCCCCHH
EVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQINYMSTLLAAIVGLLMLA
HHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNVTIRMPDEVPPNISQVFKDVI
CCHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHH
PFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSAADGYLGITIIFGAFAFFWFVGI
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
HGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILTSGTQMFIVTMGGTGATLVVPFMFM
CCCHHHHHHHHHHEECCCCEEHHHHHCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHH
WLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLVLNPIFFIPFIFAPIANVWIFKFFIET
HHHHCHHHCCCCCHHHCHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGMNSFTANLPWTTPAPLGLVLGTNFQVLSFILAALLIVVDVVIYYPFLKVYDEQILEEE
HCCCCEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSGKSNDELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLANA
HCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCHHHHHHH
LNKAAAEYNVPVKAAAGGYGAHREMLPEFDLVILAPQVASNFEDMKAETDKLGIKLAKTE
HHHHHHHCCCCEEECCCCCCHHHHHCCCCCEEEECCHHHCCHHHHHHHHHHHCEEEEECC
GAQYIKLTRDGKGALAFVQEQFD
CCEEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIFILIAFVPNSWGFKWSD
CCHHHHHHHCCCHHHHHHCCCEEEEEHHCCHHCCCHHHHHHHHHHHHHCCCCCCCCCCHH
EVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQINYMSTLLAAIVGLLMLA
HHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNVTIRMPDEVPPNISQVFKDVI
CCHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHH
PFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSAADGYLGITIIFGAFAFFWFVGI
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
HGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILTSGTQMFIVTMGGTGATLVVPFMFM
CCCHHHHHHHHHHEECCCCEEHHHHHCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHH
WLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLVLNPIFFIPFIFAPIANVWIFKFFIET
HHHHCHHHCCCCCHHHCHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGMNSFTANLPWTTPAPLGLVLGTNFQVLSFILAALLIVVDVVIYYPFLKVYDEQILEEE
HCCCCEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSGKSNDELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLANA
HCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCHHHHHHH
LNKAAAEYNVPVKAAAGGYGAHREMLPEFDLVILAPQVASNFEDMKAETDKLGIKLAKTE
HHHHHHHCCCCEEECCCCCCHHHHHCCCCCEEEECCHHHCCHHHHHHHHHHHCEEEEECC
GAQYIKLTRDGKGALAFVQEQFD
CCEEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2125052 [H]