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The map label for this gene is mscL

Identifier: 225858802

GI number: 225858802

Start: 967801

End: 968178

Strand: Reverse

Name: mscL

Synonym: SP70585_1049

Alternate gene names: 225858802

Gene position: 968178-967801 (Counterclockwise)

Preceding gene: 225858803

Following gene: 225858800

Centisome position: 44.32

GC content: 37.3

Gene sequence:

>378_bases
ATGTTAAAAAATCTAAAATCGTTCTTGCTTCGAGGAAATGTTATTGACCTTGCTGTCGGTGTTGTCATTGCCTCTGCTTT
TGGTGCTATCGTTACTTCACTTGTAAACGACATTATCACTCCTCTTATTTTAAATCCCGCTTTGAAAGCTGCTAAAGTTG
AACGTATCGCTCAACTTTCTTGGCATGGAGTCGGCTATGGTAACTTCTTAAGTGCTATTATCAATTTTATCTTTGTGGGT
ACCGCCCTCTTCTTTATTATCAAGGGCATTGAAAAAGCACAGAAACTGACTGGCATAAAGAAAGAAAAAACTGCCGAAAA
AAAACCAACCGAATTGGAAGTCCTTCAAGAAATAAAAGCTCTCCTTGAGAAAAAATAA

Upstream 100 bases:

>100_bases
CTATATTTTTAGAAAATAATGCTCCATTTATGAGCCTTTTAATTGCCTTCTATAATGATTTAGGATACAATGAAACACGA
AACATTGAAAGGAAAACAGT

Downstream 100 bases:

>100_bases
TCGATTAAAAGGATCTAGCGCAAAGCTAAATCCTTTTTTCTTTACTCTTTGGGTAACTTGCCTGCTTTTTCAAGCATTTT
TTTGATAAGATAAGGCATCT

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLILNPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVG
TALFFIIKGIEKAQKLTGIKKEKTAEKKPTELEVLQEIKALLEKK

Sequences:

>Translated_125_residues
MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLILNPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVG
TALFFIIKGIEKAQKLTGIKKEKTAEKKPTELEVLQEIKALLEKK
>Mature_125_residues
MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLILNPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVG
TALFFIIKGIEKAQKLTGIKKEKTAEKKPTELEVLQEIKALLEKK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=133, Percent_Identity=42.8571428571429, Blast_Score=75, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 13617; Mature: 13617

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLILNPALKAAKVERIAQLS
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKEKTAEKKPTELEVLQEIKA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHH
LLEKK
HHCCC
>Mature Secondary Structure
MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLILNPALKAAKVERIAQLS
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKEKTAEKKPTELEVLQEIKA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHH
LLEKK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA