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The map label for this gene is pepT

Identifier: 225858800

GI number: 225858800

Start: 965288

End: 966511

Strand: Reverse

Name: pepT

Synonym: SP70585_1047

Alternate gene names: 225858800

Gene position: 966511-965288 (Counterclockwise)

Preceding gene: 225858802

Following gene: 225858781

Centisome position: 44.24

GC content: 42.48

Gene sequence:

>1224_bases
ATGACTTATCCAAATCTCTTGGACCGCTTCTTAACCTACGTTAAGGTCAACACGCGCTCTGATGAACACTCTACTACTAC
TCCAAGTACACAGAGTCAGGTTGACTTCGCAACAAATGTCCTAATTCCTGAAATGAAACGTGTTGGACTGCAAAATGTTT
ACTATCTACCGAATGGTTTTGCTATTGGAACCTTGCCAGCCAACGATCCGTCTTTAACACGTAAGATTGGTTTTATATCG
CACATGGATACTGCTGATTTTAATGCTGAAGGAGTCAATCCACAGGTAATTGAAAACTACGATGGTGGTGTGATTGAACT
AGGGAATTCTGGTTTCAAACTCGATCCAGCTGACTTCAAGAGTCTTGAAAAATATCCAGGACAAACGCTCATCACAACAG
ATGGAACAAGCTTGCTAGGTGCTGATGACAAGTCAGGAATTGCTGAAATTATGACAGCCATTGAATATCTAACTGCTCAT
CCTGAAATTAAGCACTGTGAGATTCGTGTTGGTTTTGGTCCAGATGAAGAAATCGGTGTTGGTGCCAATAAATTTGATGC
AGAAGATTTTGATGTGGATTTTGCCTACACTGTTGATGGTGGTCCACTAGGTGAACTTCAGTACGAGACTTTCTCAGCCG
CTGGTGCTGAATTGCATTTCCAAGGTCGTAATGTCCACCCTGGTACTGCCAAAGGGCAGATGGTCAATGCCCTTCAGCTA
GCAATTGATTTTCATAATCAACTTCCAGAAAATGACCGACCTGAGTTAACTGAAGGTTACCAAGGTTTTTACCATCTAAT
GGATGTGACAGGTAGTGTTGAGGAGGCGCGTGCAAGCTACATCATTCGTGATTTTGAAAAAGATGCCTTTGAAGCGCGTA
AAGCATCCATGCAATCTATCGCTGATAAGATGAATGAAGAACTTGGGAGCGACCGTGTCACTCTCAACTTGACAGACCAG
TACTACAATATGAAAGAAGTCATTGAAAAAGATATGACTCCAATTACCATTGCTAAAGCCGTTATGGAAGATCTAGGTAT
CACGCCTATTATCGAACCAATCCGGGGTGGAACAGACGGCTCTAAGATTTCCTTTATGGGAATCCCAACTCCGAATATCT
TTGCAGGTGGCGAAAATATGCACGGACGTTTTGAATACGTTAGCCTTCAGACTATGGAACGTGCAGTTGATACCATCATT
GGCATCGTAGCTTATAAAGGCTAA

Upstream 100 bases:

>100_bases
TAAGTAAACGCTAACATAGGGACATTCTCAATCTTCTGTCTTTTGTTTTTCTCTTCTTTCTATGATACAATGGAAAAAAT
AAATTCAAAAGGAGTTTTTT

Downstream 100 bases:

>100_bases
AAAGACGAGGTAGCTCAGCTACTTCGCCTTTCTTTTTATTCTACTGGTTTTTCTTGATTTCCAGTAGTTGTAGAAGATTC
TGTTGTTTCATTTTCTGAAG

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPEMKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFIS
HMDTADFNAEGVNPQVIENYDGGVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAELHFQGRNVHPGTAKGQMVNALQL
AIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEARASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQ
YYNMKEVIEKDMTPITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTMERAVDTII
GIVAYKG

Sequences:

>Translated_407_residues
MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPEMKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFIS
HMDTADFNAEGVNPQVIENYDGGVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAELHFQGRNVHPGTAKGQMVNALQL
AIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEARASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQ
YYNMKEVIEKDMTPITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTMERAVDTII
GIVAYKG
>Mature_406_residues
TYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPEMKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISH
MDTADFNAEGVNPQVIENYDGGVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAHP
EIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAELHFQGRNVHPGTAKGQMVNALQLA
IDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEARASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQY
YNMKEVIEKDMTPITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTMERAVDTIIG
IVAYKG

Specific function: Cleaves the N-terminal amino acid of tripeptides

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family

Homologues:

Organism=Escherichia coli, GI1787372, Length=400, Percent_Identity=50, Blast_Score=384, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPT_STRP7 (C1C6Y4)

Other databases:

- EMBL:   CP000918
- RefSeq:   YP_002740310.1
- ProteinModelPortal:   C1C6Y4
- SMR:   C1C6Y4
- EnsemblBacteria:   EBSTRT00000074953
- GeneID:   7683767
- GenomeReviews:   CP000918_GR
- KEGG:   snm:SP70585_1047
- GeneTree:   EBGT00050000027629
- ProtClustDB:   PRK05469
- BioCyc:   SPNE488221:SP70585_1047-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00550
- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161
- PIRSF:   PIRSF037215
- TIGRFAMs:   TIGR01882

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.4.11.4

Molecular weight: Translated: 44776; Mature: 44645

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 83-83 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPEMKRVGLQNVYYLPNGF
CCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCE
AIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDGGVIELGNSGFKLDPADFK
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCEECHHHHH
SLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAHPEIKHCEIRVGFGPDEEIGV
HHHHCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
GANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAELHFQGRNVHPGTAKGQMVNALQL
CCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHH
AIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEARASYIIRDFEKDAFEARKASMQSI
EEHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTPITIAKAVMEDLGITPIIEPIRGGTDG
HHHHHHHHCCCEEEEEECHHHCCHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHCCCCCC
SKISFMGIPTPNIFAGGENMHGRFEYVSLQTMERAVDTIIGIVAYKG
CEEEEEECCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
TYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPEMKRVGLQNVYYLPNGF
CCHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCE
AIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDGGVIELGNSGFKLDPADFK
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCEECHHHHH
SLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAHPEIKHCEIRVGFGPDEEIGV
HHHHCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
GANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAELHFQGRNVHPGTAKGQMVNALQL
CCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHH
AIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEARASYIIRDFEKDAFEARKASMQSI
EEHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTPITIAKAVMEDLGITPIIEPIRGGTDG
HHHHHHHHCCCEEEEEECHHHCCHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHCCCCCC
SKISFMGIPTPNIFAGGENMHGRFEYVSLQTMERAVDTIIGIVAYKG
CEEEEEECCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA