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The map label for this gene is tcyJ [H]

Identifier: 225858454

GI number: 225858454

Start: 613953

End: 614753

Strand: Reverse

Name: tcyJ [H]

Synonym: SP70585_0681

Alternate gene names: 225858454

Gene position: 614753-613953 (Counterclockwise)

Preceding gene: 225858466

Following gene: 225858445

Centisome position: 28.14

GC content: 41.32

Gene sequence:

>801_bases
ATGAAAAAATTTAGCCTATTACTAGCTATCCTACCATTTTTGGTTGCCTGTGGGAATCAAGCTACACCCAAAGAGTCCAG
CTCTCAAAAGACAATCGTCGTTGCTACAGCTGGCGATGTGCCACCGTTTGACTACGAAGATAAAGGTAATCTGACAGGCT
TCGATATCGAAGTTCTAAAGGCAGTAGATGAAAAACTCAGCGACTACGAGATTCAATTTCAAAGAACCGCCTGGGAAAGT
ATCTTCCCAGGACTTGATTCTGGTCACTATCAAGCTGCGGCCAATAACTTGAGTTACACAAAAGAGCGCGCTGAAAAATA
CCTCTACTCACTTCCGATTTCCAACAATCCCCTCGTCCTTGTCAGCAACAAGAAAAATCCTTTGACTTCTCTTGACCAGA
TCGCTGGTAAAACAACACAAGAGGATACCGGAACTTCTAACGCTCAATTCATCAATAACTGGAATCAGAAACACACTGAT
AATCCCGCTACAATTGATTTTTCTGGTGAGGATATTGGTAAACGAATCCTAGACCTTGCTAACGGAGAGTTTGATTTCCT
AGTTTTTGACAAGGTATCCGTTCAAAAGATTATCAAGGACCGTGGTTTAGACCTCTCAGTCGTTGATTTACCTTCTACAG
ATAGCCCCAGCAACTATATCGTTTTCTCAAGTGACCAAAAAGAGTTTAAAGAGAAATTTGATAAAGCGCTCAAAGAACTC
TATCAAGACGGAACCCTTGAAAAACTCAGCAATACCTATCTAGGTGGTTCTTACCTCCCAGATCAATCTCAGTTACAATA
A

Upstream 100 bases:

>100_bases
ATGATTAAGTTTTTCTATCACAGACATAAAAGATATATATTGGTTTATCAGGAAAGTTTCTGATAAACTAGCAAGTACTT
TACATTTGAATGGAGACAGT

Downstream 100 bases:

>100_bases
CACTCTTCGAAAATCTCTTCAAACCACGTCAGCTTTATCTGCAACCTCAAAGCTGTGCTTTGAGCAACCTACGGCTAGCT
TCCTAGTTTGCTCTTTGATT

Product: ABC transporter substrate-binding protein - glutamine transport/Major cell binding factor

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKKFSLLLAILPFLVACGNQATPKESSSQKTIVVATAGDVPPFDYEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWES
IFPGLDSGHYQAAANNLSYTKERAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSTDSPSNYIVFSSDQKEFKEKFDKALKEL
YQDGTLEKLSNTYLGGSYLPDQSQLQ

Sequences:

>Translated_266_residues
MKKFSLLLAILPFLVACGNQATPKESSSQKTIVVATAGDVPPFDYEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWES
IFPGLDSGHYQAAANNLSYTKERAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSTDSPSNYIVFSSDQKEFKEKFDKALKEL
YQDGTLEKLSNTYLGGSYLPDQSQLQ
>Mature_266_residues
MKKFSLLLAILPFLVACGNQATPKESSSQKTIVVATAGDVPPFDYEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWES
IFPGLDSGHYQAAANNLSYTKERAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSTDSPSNYIVFSSDQKEFKEKFDKALKEL
YQDGTLEKLSNTYLGGSYLPDQSQLQ

Specific function: Part of the ABC transporter complex tcyJKLMN involved in L-cystine import. Is also involved in cystathionine, djenkolate, and S-methylcysteine transport [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=234, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787085, Length=233, Percent_Identity=24.8927038626609, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788228, Length=229, Percent_Identity=24.0174672489083, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788649, Length=263, Percent_Identity=25.0950570342205, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29615; Mature: 29615

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFSLLLAILPFLVACGNQATPKESSSQKTIVVATAGDVPPFDYEDKGNLTGFDIEVLK
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
AVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKERAEKYLYSLPISNNPLVL
HHHHHCCCEEEEEHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHEEECCCCCCCEEE
VSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTDNPATIDFSGEDIGKRILDLA
EECCCCCCHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHC
NGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSTDSPSNYIVFSSDQKEFKEKFDKALKEL
CCCEEEEEECHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
YQDGTLEKLSNTYLGGSYLPDQSQLQ
HCCCCHHHHHHCCCCCCCCCCHHCCC
>Mature Secondary Structure
MKKFSLLLAILPFLVACGNQATPKESSSQKTIVVATAGDVPPFDYEDKGNLTGFDIEVLK
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
AVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKERAEKYLYSLPISNNPLVL
HHHHHCCCEEEEEHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHEEECCCCCCCEEE
VSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTDNPATIDFSGEDIGKRILDLA
EECCCCCCHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHC
NGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSTDSPSNYIVFSSDQKEFKEKFDKALKEL
CCCEEEEEECHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
YQDGTLEKLSNTYLGGSYLPDQSQLQ
HCCCCHHHHHHCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]