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The map label for this gene is mepA [H]

Identifier: 225686801

GI number: 225686801

Start: 1009887

End: 1010909

Strand: Direct

Name: mepA [H]

Synonym: BMEA_B1032

Alternate gene names: 225686801

Gene position: 1009887-1010909 (Clockwise)

Preceding gene: 225686800

Following gene: 225686802

Centisome position: 85.19

GC content: 61.09

Gene sequence:

>1023_bases
TTGCGGTTTGGCACATCACACCGCATAATTTCACAGTCGCGCTCGATTGATTCGGGCAGGAATTCGGGAAATCGATCCAT
GGCTCAGAAATCCGGCTCCAAATTCTGCTCTCGTCTGGTTCTCGCCGCTTCGCTGGCGGCAATGATCGCGCCCGGCTTCT
TGCCGGTGGCTATGGCGGAAGACATGCCCGCCAAACAGGCTTTCGGTGCGGCGCAATTGCCTATGCTGGCGCAGCAGCCG
CAATCCATCGGCTTTTATGCCAAGGGCTGCCTTGCCGGTGCCGTGGCGCTGCCGACCGACGGCCCCAACTGGCAGGTCAT
GCGCCTTTCGCGCAACCGCCGCTGGGGCCACCCGCGCATGATCGCGCTTCTGGAAAAGCTGTCGCACGACGCGGCCAGGG
ACGGATGGTCGGGCTTGCTGGTCGGGGATATTTCCCAGCCGCGCGGCGGGCCGATGCTGACTGGCCACGCCTCGCATCAG
GTGGGCCTTGATGCCGATATCTGGCTGACGCCCATGCCCAAAAAACGCTTCACCAATGCCGAACGTGAGAGCGTTTCCGC
CGTTTCCATGCTGAAACCGGATTCGCTCTATGTGGACCCGAAAAAGTGGACACCTGCCCGCACCGCGCTTTTGAAACATG
CCGCCAGCTATCCGGAAGTGGAACGCATCTTCGTGCATCCCGGCATCAAGAAACAACTCTGCGACACGGTGACAGGCGAC
AGAAGCTGGCTCGGCAAGGTTCGCCCCTATTGGGGCCACTTCTACCATTTCCATGTGCGCCTCACCTGCCAGCCGGGTTC
GCCCGAATGCAAGCCCCAACCCAAGGTCGCGCCCGGCGACGGTTGCGACAAATCGCTTGCATGGTGGTTTACGGATGAGC
CGTGGAAGCCCGCCAAGCCATCCGGCAAACCTGCAAAGAAGCCAAAGCCGGTGATGGTTTCCGATTTGCCGAAAGCCTGT
GCCGCCGTGTTGAACGGGCCGGCGCCGAACTCTATCGCAGACGTCACCTACGGCACAAAATAG

Upstream 100 bases:

>100_bases
CGTTTCCAATGAGCGCATCAAGACTTTGGGCTACGAATTTACCTATCCCGACTACAAGACCGCCTTCTCGGCCATGTGGG
CTGCGGACAACTGGCGCTGA

Downstream 100 bases:

>100_bases
AGCTTTTCGCGCAAGCATGTCCGTCCAGCGCAATTACAGGGCTTGACGCGCACTGACACAATCTGCTTTTCAGCAGCGCG
TGGCTCCGTTATAGATTTAA

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MRFGTSHRIISQSRSIDSGRNSGNRSMAQKSGSKFCSRLVLAASLAAMIAPGFLPVAMAEDMPAKQAFGAAQLPMLAQQP
QSIGFYAKGCLAGAVALPTDGPNWQVMRLSRNRRWGHPRMIALLEKLSHDAARDGWSGLLVGDISQPRGGPMLTGHASHQ
VGLDADIWLTPMPKKRFTNAERESVSAVSMLKPDSLYVDPKKWTPARTALLKHAASYPEVERIFVHPGIKKQLCDTVTGD
RSWLGKVRPYWGHFYHFHVRLTCQPGSPECKPQPKVAPGDGCDKSLAWWFTDEPWKPAKPSGKPAKKPKPVMVSDLPKAC
AAVLNGPAPNSIADVTYGTK

Sequences:

>Translated_340_residues
MRFGTSHRIISQSRSIDSGRNSGNRSMAQKSGSKFCSRLVLAASLAAMIAPGFLPVAMAEDMPAKQAFGAAQLPMLAQQP
QSIGFYAKGCLAGAVALPTDGPNWQVMRLSRNRRWGHPRMIALLEKLSHDAARDGWSGLLVGDISQPRGGPMLTGHASHQ
VGLDADIWLTPMPKKRFTNAERESVSAVSMLKPDSLYVDPKKWTPARTALLKHAASYPEVERIFVHPGIKKQLCDTVTGD
RSWLGKVRPYWGHFYHFHVRLTCQPGSPECKPQPKVAPGDGCDKSLAWWFTDEPWKPAKPSGKPAKKPKPVMVSDLPKAC
AAVLNGPAPNSIADVTYGTK
>Mature_340_residues
MRFGTSHRIISQSRSIDSGRNSGNRSMAQKSGSKFCSRLVLAASLAAMIAPGFLPVAMAEDMPAKQAFGAAQLPMLAQQP
QSIGFYAKGCLAGAVALPTDGPNWQVMRLSRNRRWGHPRMIALLEKLSHDAARDGWSGLLVGDISQPRGGPMLTGHASHQ
VGLDADIWLTPMPKKRFTNAERESVSAVSMLKPDSLYVDPKKWTPARTALLKHAASYPEVERIFVHPGIKKQLCDTVTGD
RSWLGKVRPYWGHFYHFHVRLTCQPGSPECKPQPKVAPGDGCDKSLAWWFTDEPWKPAKPSGKPAKKPKPVMVSDLPKAC
AAVLNGPAPNSIADVTYGTK

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=245, Percent_Identity=42.8571428571429, Blast_Score=188, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 37052; Mature: 37052

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFGTSHRIISQSRSIDSGRNSGNRSMAQKSGSKFCSRLVLAASLAAMIAPGFLPVAMAE
CCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHC
DMPAKQAFGAAQLPMLAQQPQSIGFYAKGCLAGAVALPTDGPNWQVMRLSRNRRWGHPRM
CCCHHHHCCCHHCCHHHCCCCCCCHHHHHHHHCEEECCCCCCCEEEEEEECCCCCCCHHH
IALLEKLSHDAARDGWSGLLVGDISQPRGGPMLTGHASHQVGLDADIWLTPMPKKRFTNA
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCEEECCCCHHHHCCC
ERESVSAVSMLKPDSLYVDPKKWTPARTALLKHAASYPEVERIFVHPGIKKQLCDTVTGD
HHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCC
RSWLGKVRPYWGHFYHFHVRLTCQPGSPECKPQPKVAPGDGCDKSLAWWFTDEPWKPAKP
HHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
SGKPAKKPKPVMVSDLPKACAAVLNGPAPNSIADVTYGTK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCC
>Mature Secondary Structure
MRFGTSHRIISQSRSIDSGRNSGNRSMAQKSGSKFCSRLVLAASLAAMIAPGFLPVAMAE
CCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHC
DMPAKQAFGAAQLPMLAQQPQSIGFYAKGCLAGAVALPTDGPNWQVMRLSRNRRWGHPRM
CCCHHHHCCCHHCCHHHCCCCCCCHHHHHHHHCEEECCCCCCCEEEEEEECCCCCCCHHH
IALLEKLSHDAARDGWSGLLVGDISQPRGGPMLTGHASHQVGLDADIWLTPMPKKRFTNA
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCEEECCCCHHHHCCC
ERESVSAVSMLKPDSLYVDPKKWTPARTALLKHAASYPEVERIFVHPGIKKQLCDTVTGD
HHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCC
RSWLGKVRPYWGHFYHFHVRLTCQPGSPECKPQPKVAPGDGCDKSLAWWFTDEPWKPAKP
HHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
SGKPAKKPKPVMVSDLPKACAAVLNGPAPNSIADVTYGTK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]