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The map label for this gene is 225686782

Identifier: 225686782

GI number: 225686782

Start: 993415

End: 994245

Strand: Reverse

Name: 225686782

Synonym: BMEA_B1013

Alternate gene names: NA

Gene position: 994245-993415 (Counterclockwise)

Preceding gene: 225686784

Following gene: 225686774

Centisome position: 83.87

GC content: 57.04

Gene sequence:

>831_bases
ATGGAGAAGTCCGGCTGGACGCCGTTCCCGAATTCAGAGGAAGCGGTCAAGCAGGCGCGAACCGTACCGCAGACACCCCA
GACCGAGGCACCCGCCTACCGTCTTGCCTTCATGGACGATGAGTTCATGACCCGTCGCGACCTGCGCCCGATCCGGTTGC
AGCTTGAGCTGCTCAAGCCGGAAATGATTCTTGCCGATCGCGGTATCAAATCCACCGTCGTCATGTTTGGTGGCGCGCGC
ATTCCCGAGCCGGGCGGCGAGGCATGGGCTGCCAAAAATGAGGTTCAGAAGAAGAACCTTCAGGCCAATTCGCATTATTA
TGAAGAAGCGCGCAAATTCGCGCGGCTCTGCTCGGAATATTCCGCAACCACCTATTACCGCGAATTCATCGTGGTGACGG
GCGGGGGGCCGGGCGTCATGGAGGCGGGCAATCGCGGGGCTGCCGATGTGGGTGCGCCGACCATCGGTCTCAATATCGTA
TTGCCGCATGAACAGGCCCCCAATGCCTATGTCACGCCGGATCTCTGCTTCAACTTCCATTATTTCGCCATCCGCAAAAT
GCATTTTCTGATGCGCGCGAAGGCGGTCTGCGTCTTCCCCGGCGGGTTTGGCACGATGGATGAATTGTTTGAGGCGATGA
CGCTGATCCAGACCAACCGCATGGAGCGGATGCCGCTTATCCTGTTTGGCAAGGAATTCTGGACCAAGGCGATCAATATC
GAGTTTCTGGCCGAGCAGGGCACGATTTCGCCAGCCGATATAGAACTCTTGAACTTTGTCGAAACCGCCGATGAAGCCTG
GGGCCATATCAAGGATTTTTATAAGCTCTGA

Upstream 100 bases:

>100_bases
GTTCTCGGACAATGTTTGCCGTGTTTGAGACAGATTAAGCATGGCGCCAGAATAAGCTTGGTATTGAAATTGTAAGGAGC
AGGTGAGAAGCATGAACCCG

Downstream 100 bases:

>100_bases
TTGCCCGGAATATTCCACTTGATTTGCCCAGCCGAAAGGTTGGGCATTTCTATTCTGTTGCTTATCGGTCCGGGCTTATT
TGTTCCAGAAACCGGGTCAG

Product: hypothetical protein

Products: (2S)-2-isopropyl-3-oxosuccinate; NADH; H+

Alternate protein names: None

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MEKSGWTPFPNSEEAVKQARTVPQTPQTEAPAYRLAFMDDEFMTRRDLRPIRLQLELLKPEMILADRGIKSTVVMFGGAR
IPEPGGEAWAAKNEVQKKNLQANSHYYEEARKFARLCSEYSATTYYREFIVVTGGGPGVMEAGNRGAADVGAPTIGLNIV
LPHEQAPNAYVTPDLCFNFHYFAIRKMHFLMRAKAVCVFPGGFGTMDELFEAMTLIQTNRMERMPLILFGKEFWTKAINI
EFLAEQGTISPADIELLNFVETADEAWGHIKDFYKL

Sequences:

>Translated_276_residues
MEKSGWTPFPNSEEAVKQARTVPQTPQTEAPAYRLAFMDDEFMTRRDLRPIRLQLELLKPEMILADRGIKSTVVMFGGAR
IPEPGGEAWAAKNEVQKKNLQANSHYYEEARKFARLCSEYSATTYYREFIVVTGGGPGVMEAGNRGAADVGAPTIGLNIV
LPHEQAPNAYVTPDLCFNFHYFAIRKMHFLMRAKAVCVFPGGFGTMDELFEAMTLIQTNRMERMPLILFGKEFWTKAINI
EFLAEQGTISPADIELLNFVETADEAWGHIKDFYKL
>Mature_276_residues
MEKSGWTPFPNSEEAVKQARTVPQTPQTEAPAYRLAFMDDEFMTRRDLRPIRLQLELLKPEMILADRGIKSTVVMFGGAR
IPEPGGEAWAAKNEVQKKNLQANSHYYEEARKFARLCSEYSATTYYREFIVVTGGGPGVMEAGNRGAADVGAPTIGLNIV
LPHEQAPNAYVTPDLCFNFHYFAIRKMHFLMRAKAVCVFPGGFGTMDELFEAMTLIQTNRMERMPLILFGKEFWTKAINI
EFLAEQGTISPADIELLNFVETADEAWGHIKDFYKL

Specific function: Unknown

COG id: COG1611

COG function: function code R; Predicted Rossmann fold nucleotide-binding protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.1.1.85

Molecular weight: Translated: 31174; Mature: 31174

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSGWTPFPNSEEAVKQARTVPQTPQTEAPAYRLAFMDDEFMTRRDLRPIRLQLELLKP
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHCCCCEEEEEECCCC
EMILADRGIKSTVVMFGGARIPEPGGEAWAAKNEVQKKNLQANSHYYEEARKFARLCSEY
EEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SATTYYREFIVVTGGGPGVMEAGNRGAADVGAPTIGLNIVLPHEQAPNAYVTPDLCFNFH
HHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEECHHHHHHHH
YFAIRKMHFLMRAKAVCVFPGGFGTMDELFEAMTLIQTNRMERMPLILFGKEFWTKAINI
HHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCCHHCCCEEEECHHHHHHHCCE
EFLAEQGTISPADIELLNFVETADEAWGHIKDFYKL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKSGWTPFPNSEEAVKQARTVPQTPQTEAPAYRLAFMDDEFMTRRDLRPIRLQLELLKP
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHCCCCEEEEEECCCC
EMILADRGIKSTVVMFGGARIPEPGGEAWAAKNEVQKKNLQANSHYYEEARKFARLCSEY
EEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SATTYYREFIVVTGGGPGVMEAGNRGAADVGAPTIGLNIVLPHEQAPNAYVTPDLCFNFH
HHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEECHHHHHHHH
YFAIRKMHFLMRAKAVCVFPGGFGTMDELFEAMTLIQTNRMERMPLILFGKEFWTKAINI
HHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCCHHCCCEEEECHHHHHHHCCE
EFLAEQGTISPADIELLNFVETADEAWGHIKDFYKL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (2R,3S)-3-isopropylmalate; NAD+

Specific reaction: (2R,3S)-3-isopropylmalate + NAD+ = (2S)-2-isopropyl-3-oxosuccinate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA