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The map label for this gene is gatA [H]

Identifier: 225686762

GI number: 225686762

Start: 974694

End: 976082

Strand: Reverse

Name: gatA [H]

Synonym: BMEA_B0992

Alternate gene names: 225686762

Gene position: 976082-974694 (Counterclockwise)

Preceding gene: 225686763

Following gene: 225686760

Centisome position: 82.33

GC content: 60.48

Gene sequence:

>1389_bases
ATGTCACATCTTGCTGATCTGTCGGCTGTGGAACTTGTCTCGTCCTATAAGGCAAAGGCGCTTTCGCCGGTGGAAGTCAC
GGAAGCCGTTATCGGGCGTATCGAAGCTTATGAGCCAAAGCTGAATGCGCTCTGGGCCTATGATCCTGAAGCAGCGCGCC
TGGCTGCGAAAGCTTCGGAAGCGCGTTGGGCAAAGGGCGAACCGGCTGGTCCCATCGACGGTGTGCCGCTGACGATCAAG
GAAAATATTGCAACGAAAGGAACGCCAGTGCCGCTTGGCTGTGCGGCCCTGCCGTTAAAGCCTGCCGCCGCCGATGCACC
GCCTGCGGCGCGCACGCGCGAGGCGGGGGGCGTGCTGCTTGCCAAGACCACCATGCCCGATCTCGGCATGTTGTCGTCGG
GCCTGTCGAGTTTTCACAAGCTGGCCCGCAATCCGTGGGATATTGCCACCAATCCCGGCGGTTCAAGCGCTGGCGCGGGA
AGTGCCGCCGCCGCCGGTTACGGCCCGCTCCATATCGGCACGGATATTGGCGGCTCCGTCCGCCTGCCTGCCGGCTGGTG
CGGTCTGGTCGGCCTGAAGCCATCCTTCGGGCGCATTCCCATCGATCCGCCGTTTCTGGGGCGCGTTGCCGGGCCTATGA
CGCGCACTGTGGCCGATAACGCGCTTTATATGTCGGTGCTGTCGCGGCCGGATCGGCGTGATGGCATGAGCCTGCCCTAT
CAGGATATCGACTGGATGAACCTCGACATCGAAGTGAAGGGCTTGAAGATCGGCCTTTGGCTCGATGCCGGTTTTGACGA
GCCGGTGGGGGGGGGAACCCGTGCCGCCGTGGAAGCCGCAGCCAAACTGTTTGCCGATGCAGGCGCGATCATCGAGCCGG
TGGCGCCATTCCTCAATCGCACCATGATCGATGGTCTGGATCGTTTCTGGCGCGCGCGTTCCTGGTCGGATATCAAAAAG
ATGACGCCGGAAAATCAGGCAAAGATATTGCCCTATATCTACCAGTGGACGGAAACGGCCGAAGGGCTTTCTGGCGAGGA
AGCCTATCTTGGTTTTGCCCAGATGGACGCCATGCGCAATGCGGCGCTTGCCGCATCGAAGGACTTCGATTTCATCATTT
CGCCAACCGCGCCGATGCCGACCTATCCGGCGGAATGGGCCTCTCCGGTCAATGATCCGCAACGCCCGTTCGAGCATATT
GCCTTCACCGTTGCGATGAATATGTCGGAACAGCCGGCCATTGCGATCAATTGTGGTTATACGGCCAAGGGCCTGCCCAT
TGGCTTGCAGATTTTCGGCCAGCGTTTCGATGATCTGGGTGTGTTGCGTCTTGCCAAAGCTTACGAAGATATGCGGCCGG
TGCAGAGGCCGTGGCCCGTTATTGCGTGA

Upstream 100 bases:

>100_bases
GCGCTGGTCGATGCCGTGCCAAAGCCTTTTTCCGGACGCCGCAAGCGGGCGCGCCGCCTCAATCCCATGAATAGCCTGCC
AGAATAAATAAGGGAACATC

Downstream 100 bases:

>100_bases
TCGCCAAATCCCGCTCCTCATCCTGGGGAGGCGCGAAGCGCCTTCTCGAAGGGCGAGGCACTGAATGCGGCCATTTTACT
TCTTTGCCCGTAGCGGCCCA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSHLADLSAVELVSSYKAKALSPVEVTEAVIGRIEAYEPKLNALWAYDPEAARLAAKASEARWAKGEPAGPIDGVPLTIK
ENIATKGTPVPLGCAALPLKPAAADAPPAARTREAGGVLLAKTTMPDLGMLSSGLSSFHKLARNPWDIATNPGGSSAGAG
SAAAAGYGPLHIGTDIGGSVRLPAGWCGLVGLKPSFGRIPIDPPFLGRVAGPMTRTVADNALYMSVLSRPDRRDGMSLPY
QDIDWMNLDIEVKGLKIGLWLDAGFDEPVGGGTRAAVEAAAKLFADAGAIIEPVAPFLNRTMIDGLDRFWRARSWSDIKK
MTPENQAKILPYIYQWTETAEGLSGEEAYLGFAQMDAMRNAALAASKDFDFIISPTAPMPTYPAEWASPVNDPQRPFEHI
AFTVAMNMSEQPAIAINCGYTAKGLPIGLQIFGQRFDDLGVLRLAKAYEDMRPVQRPWPVIA

Sequences:

>Translated_462_residues
MSHLADLSAVELVSSYKAKALSPVEVTEAVIGRIEAYEPKLNALWAYDPEAARLAAKASEARWAKGEPAGPIDGVPLTIK
ENIATKGTPVPLGCAALPLKPAAADAPPAARTREAGGVLLAKTTMPDLGMLSSGLSSFHKLARNPWDIATNPGGSSAGAG
SAAAAGYGPLHIGTDIGGSVRLPAGWCGLVGLKPSFGRIPIDPPFLGRVAGPMTRTVADNALYMSVLSRPDRRDGMSLPY
QDIDWMNLDIEVKGLKIGLWLDAGFDEPVGGGTRAAVEAAAKLFADAGAIIEPVAPFLNRTMIDGLDRFWRARSWSDIKK
MTPENQAKILPYIYQWTETAEGLSGEEAYLGFAQMDAMRNAALAASKDFDFIISPTAPMPTYPAEWASPVNDPQRPFEHI
AFTVAMNMSEQPAIAINCGYTAKGLPIGLQIFGQRFDDLGVLRLAKAYEDMRPVQRPWPVIA
>Mature_461_residues
SHLADLSAVELVSSYKAKALSPVEVTEAVIGRIEAYEPKLNALWAYDPEAARLAAKASEARWAKGEPAGPIDGVPLTIKE
NIATKGTPVPLGCAALPLKPAAADAPPAARTREAGGVLLAKTTMPDLGMLSSGLSSFHKLARNPWDIATNPGGSSAGAGS
AAAAGYGPLHIGTDIGGSVRLPAGWCGLVGLKPSFGRIPIDPPFLGRVAGPMTRTVADNALYMSVLSRPDRRDGMSLPYQ
DIDWMNLDIEVKGLKIGLWLDAGFDEPVGGGTRAAVEAAAKLFADAGAIIEPVAPFLNRTMIDGLDRFWRARSWSDIKKM
TPENQAKILPYIYQWTETAEGLSGEEAYLGFAQMDAMRNAALAASKDFDFIISPTAPMPTYPAEWASPVNDPQRPFEHIA
FTVAMNMSEQPAIAINCGYTAKGLPIGLQIFGQRFDDLGVLRLAKAYEDMRPVQRPWPVIA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=510, Percent_Identity=26.2745098039216, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI195972892, Length=239, Percent_Identity=31.3807531380753, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17537465, Length=495, Percent_Identity=23.030303030303, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=285, Percent_Identity=28.7719298245614, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=521, Percent_Identity=23.6084452975048, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=292, Percent_Identity=28.7671232876712, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=303, Percent_Identity=27.3927392739274, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=303, Percent_Identity=27.3927392739274, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17538254, Length=510, Percent_Identity=23.5294117647059, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32565337, Length=388, Percent_Identity=22.680412371134, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320448, Length=505, Percent_Identity=25.7425742574257, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6319685, Length=397, Percent_Identity=25.6926952141058, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=469, Percent_Identity=24.5202558635394, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550774, Length=245, Percent_Identity=33.469387755102, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648435, Length=245, Percent_Identity=33.469387755102, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648437, Length=245, Percent_Identity=33.469387755102, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356731, Length=472, Percent_Identity=23.0932203389831, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24648113, Length=491, Percent_Identity=25.6619144602851, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24644968, Length=240, Percent_Identity=30.4166666666667, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI161078093, Length=490, Percent_Identity=24.2857142857143, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648441, Length=173, Percent_Identity=35.8381502890173, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648439, Length=173, Percent_Identity=35.8381502890173, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24652985, Length=230, Percent_Identity=33.9130434782609, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922090, Length=230, Percent_Identity=33.9130434782609, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652981, Length=230, Percent_Identity=33.9130434782609, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652983, Length=230, Percent_Identity=33.9130434782609, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49251; Mature: 49120

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLADLSAVELVSSYKAKALSPVEVTEAVIGRIEAYEPKLNALWAYDPEAARLAAKASE
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHH
ARWAKGEPAGPIDGVPLTIKENIATKGTPVPLGCAALPLKPAAADAPPAARTREAGGVLL
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
AKTTMPDLGMLSSGLSSFHKLARNPWDIATNPGGSSAGAGSAAAAGYGPLHIGTDIGGSV
EECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCE
RLPAGWCGLVGLKPSFGRIPIDPPFLGRVAGPMTRTVADNALYMSVLSRPDRRDGMSLPY
ECCCCEEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
QDIDWMNLDIEVKGLKIGLWLDAGFDEPVGGGTRAAVEAAAKLFADAGAIIEPVAPFLNR
CCCCEEEEEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TMIDGLDRFWRARSWSDIKKMTPENQAKILPYIYQWTETAEGLSGEEAYLGFAQMDAMRN
HHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AALAASKDFDFIISPTAPMPTYPAEWASPVNDPQRPFEHIAFTVAMNMSEQPAIAINCGY
HHHHCCCCCCEEEECCCCCCCCCHHHCCCCCCHHHHHHHEEEEEEECCCCCCEEEEECCC
TAKGLPIGLQIFGQRFDDLGVLRLAKAYEDMRPVQRPWPVIA
CCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SHLADLSAVELVSSYKAKALSPVEVTEAVIGRIEAYEPKLNALWAYDPEAARLAAKASE
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHH
ARWAKGEPAGPIDGVPLTIKENIATKGTPVPLGCAALPLKPAAADAPPAARTREAGGVLL
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
AKTTMPDLGMLSSGLSSFHKLARNPWDIATNPGGSSAGAGSAAAAGYGPLHIGTDIGGSV
EECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCE
RLPAGWCGLVGLKPSFGRIPIDPPFLGRVAGPMTRTVADNALYMSVLSRPDRRDGMSLPY
ECCCCEEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
QDIDWMNLDIEVKGLKIGLWLDAGFDEPVGGGTRAAVEAAAKLFADAGAIIEPVAPFLNR
CCCCEEEEEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TMIDGLDRFWRARSWSDIKKMTPENQAKILPYIYQWTETAEGLSGEEAYLGFAQMDAMRN
HHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AALAASKDFDFIISPTAPMPTYPAEWASPVNDPQRPFEHIAFTVAMNMSEQPAIAINCGY
HHHHCCCCCCEEEECCCCCCCCCHHHCCCCCCHHHHHHHEEEEEEECCCCCCEEEEECCC
TAKGLPIGLQIFGQRFDDLGVLRLAKAYEDMRPVQRPWPVIA
CCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA