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The map label for this gene is lhgO [H]

Identifier: 225686760

GI number: 225686760

Start: 972232

End: 973464

Strand: Reverse

Name: lhgO [H]

Synonym: BMEA_B0990

Alternate gene names: 225686760

Gene position: 973464-972232 (Counterclockwise)

Preceding gene: 225686762

Following gene: 225686759

Centisome position: 82.11

GC content: 58.31

Gene sequence:

>1233_bases
GTGGCAGATTATGATTATTGCGTCATCGGCGGTGGCATTGTCGGGCTTGCGACCGCGAAAGCGGTGCAGGAAGCCGAGCC
GGGCGCAAGAATTATCGTTCTGGAGAAGGAAAGCGGCCTTGCCCGTCACCAGACGGGACATAATAGCGGCGTCATTCATG
CAGGCATCTATTACCAGCCGGGTTCATTGAAGGCGCGGCTTTGCCGGGCGGGCGCACAGGCCACCAAAGCGTTCTGCAAG
CAATATTCCATTCCCTTTGAGAGTTGCGGCAAACTTCTGGTCGCCACGTCCGCCTTGGAAATGGAGCGCATGGAGGCGCT
GGCCAGGCGCGCGGTGCAAAACAATATCGAATTCAGCCATCTGGACCAGCAGGCATTGCGAAAGGCGGAGCCTGCCATTT
CCGGCCTGGGGGCGCTTTTTGTTCCCGCCACCGGTATCGTGGATTACGCAAAGGTCTCGCGAGCCATGGCAGCGGAAATT
GTTGAGCGAGGCGGCATCGTGCGTCTCAATTCGCCGGTGACTGCAATCCATGAGGACGAAAAGGGTGTTGAAGTTGTTTC
GGGTGGCGAGACTGTGCGCGCTTCCAAACTCGTCGCCTGCGCCGGGCTGCAATCGGACCGTATTGCGCGGCTGGCCGGGC
TCGACATCACCCATCGCATCGTGCCCTTCCGGGGTGAGTATTATACCTTGCCGCAAACCAGGGCCGGTATCGTCAGGCAC
CTGATCTATCCTATTCCCGATCCCGACCTGCCGTTTCTTGGCATTCACCTGACGCGGACCATCGATGGCGGTGTGACGGT
TGGGCCGAATGCCGTTCTGGGTTTCTCGCGCGAAGGCTATGCCAAGGGCAGTTTCCACCCCGGCGATATCGCGGATATGT
CGTTTTTCGCAGGCTTCTGGAAAATGGCGATGAAAAACTGGCGCTCGGCAATATCCGAGTTCGGCAATTCAACCTCCCGC
GCGCGCTATCTGAAGCAGTGCCGCAAATATTGTCCGTCGCTGGAGCTTTCCGATCTGGGCGCGCCGGGGGCCGGCATTCG
TGCGCAGGCGGTGCTGGATGATGGAACGCTGGTGCATGATTTTCTGTTCAAGGAAACCGAACGGATGCTGCATGTGTGCA
ATGCGCCATCGCCAGCCGCAACCTCATCTATTCCCATCGGGCGCATGATTGCGCAAAAGCTTCTGGGGATAAACTTGCAG
GCGGAAAAGGATATATCCGTAACCGGCGAATGA

Upstream 100 bases:

>100_bases
TGTAACCAAGGCCGCAATCGTATAAGTAACAGGTAATCATCCTGTCGGGCGGCCGCAAGGCAGGGCGCTTGCGTCGGGAA
AGGCATGAGGAGGGGACTTC

Downstream 100 bases:

>100_bases
AGGTCTTTGTTTCCTATTTATTATTAGCGATTGCTTAATTTATCGATAATCTTTGTTTGGATTATGTGCCGCGCGCGGAG
GAAATATTAGCTTGACTTTC

Product: hydroxyglutarate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MADYDYCVIGGGIVGLATAKAVQEAEPGARIIVLEKESGLARHQTGHNSGVIHAGIYYQPGSLKARLCRAGAQATKAFCK
QYSIPFESCGKLLVATSALEMERMEALARRAVQNNIEFSHLDQQALRKAEPAISGLGALFVPATGIVDYAKVSRAMAAEI
VERGGIVRLNSPVTAIHEDEKGVEVVSGGETVRASKLVACAGLQSDRIARLAGLDITHRIVPFRGEYYTLPQTRAGIVRH
LIYPIPDPDLPFLGIHLTRTIDGGVTVGPNAVLGFSREGYAKGSFHPGDIADMSFFAGFWKMAMKNWRSAISEFGNSTSR
ARYLKQCRKYCPSLELSDLGAPGAGIRAQAVLDDGTLVHDFLFKETERMLHVCNAPSPAATSSIPIGRMIAQKLLGINLQ
AEKDISVTGE

Sequences:

>Translated_410_residues
MADYDYCVIGGGIVGLATAKAVQEAEPGARIIVLEKESGLARHQTGHNSGVIHAGIYYQPGSLKARLCRAGAQATKAFCK
QYSIPFESCGKLLVATSALEMERMEALARRAVQNNIEFSHLDQQALRKAEPAISGLGALFVPATGIVDYAKVSRAMAAEI
VERGGIVRLNSPVTAIHEDEKGVEVVSGGETVRASKLVACAGLQSDRIARLAGLDITHRIVPFRGEYYTLPQTRAGIVRH
LIYPIPDPDLPFLGIHLTRTIDGGVTVGPNAVLGFSREGYAKGSFHPGDIADMSFFAGFWKMAMKNWRSAISEFGNSTSR
ARYLKQCRKYCPSLELSDLGAPGAGIRAQAVLDDGTLVHDFLFKETERMLHVCNAPSPAATSSIPIGRMIAQKLLGINLQ
AEKDISVTGE
>Mature_409_residues
ADYDYCVIGGGIVGLATAKAVQEAEPGARIIVLEKESGLARHQTGHNSGVIHAGIYYQPGSLKARLCRAGAQATKAFCKQ
YSIPFESCGKLLVATSALEMERMEALARRAVQNNIEFSHLDQQALRKAEPAISGLGALFVPATGIVDYAKVSRAMAAEIV
ERGGIVRLNSPVTAIHEDEKGVEVVSGGETVRASKLVACAGLQSDRIARLAGLDITHRIVPFRGEYYTLPQTRAGIVRHL
IYPIPDPDLPFLGIHLTRTIDGGVTVGPNAVLGFSREGYAKGSFHPGDIADMSFFAGFWKMAMKNWRSAISEFGNSTSRA
RYLKQCRKYCPSLELSDLGAPGAGIRAQAVLDDGTLVHDFLFKETERMLHVCNAPSPAATSSIPIGRMIAQKLLGINLQA
EKDISVTGE

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=397, Percent_Identity=43.3249370277078, Blast_Score=303, Evalue=2e-82,
Organism=Escherichia coli, GI87082148, Length=393, Percent_Identity=53.4351145038168, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17565764, Length=404, Percent_Identity=41.3366336633663, Blast_Score=314, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24585081, Length=406, Percent_Identity=41.871921182266, Blast_Score=296, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 44065; Mature: 43934

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADYDYCVIGGGIVGLATAKAVQEAEPGARIIVLEKESGLARHQTGHNSGVIHAGIYYQP
CCCCCEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCCCEEEEEEEECC
GSLKARLCRAGAQATKAFCKQYSIPFESCGKLLVATSALEMERMEALARRAVQNNIEFSH
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEHHHHHHHHHHHHHHHHHHHHCCCCHHH
LDQQALRKAEPAISGLGALFVPATGIVDYAKVSRAMAAEIVERGGIVRLNSPVTAIHEDE
HHHHHHHHHCHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCC
KGVEVVSGGETVRASKLVACAGLQSDRIARLAGLDITHRIVPFRGEYYTLPQTRAGIVRH
CCCEEEECCCEEHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCEEECCCHHHHHHHH
LIYPIPDPDLPFLGIHLTRTIDGGVTVGPNAVLGFSREGYAKGSFHPGDIADMSFFAGFW
HHCCCCCCCCCEEEEEEEEEECCCEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHH
KMAMKNWRSAISEFGNSTSRARYLKQCRKYCPSLELSDLGAPGAGIRAQAVLDDGTLVHD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHH
FLFKETERMLHVCNAPSPAATSSIPIGRMIAQKLLGINLQAEKDISVTGE
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADYDYCVIGGGIVGLATAKAVQEAEPGARIIVLEKESGLARHQTGHNSGVIHAGIYYQP
CCCCEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCCCEEEEEEEECC
GSLKARLCRAGAQATKAFCKQYSIPFESCGKLLVATSALEMERMEALARRAVQNNIEFSH
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEHHHHHHHHHHHHHHHHHHHHCCCCHHH
LDQQALRKAEPAISGLGALFVPATGIVDYAKVSRAMAAEIVERGGIVRLNSPVTAIHEDE
HHHHHHHHHCHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCC
KGVEVVSGGETVRASKLVACAGLQSDRIARLAGLDITHRIVPFRGEYYTLPQTRAGIVRH
CCCEEEECCCEEHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCEEECCCHHHHHHHH
LIYPIPDPDLPFLGIHLTRTIDGGVTVGPNAVLGFSREGYAKGSFHPGDIADMSFFAGFW
HHCCCCCCCCCEEEEEEEEEECCCEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHH
KMAMKNWRSAISEFGNSTSRARYLKQCRKYCPSLELSDLGAPGAGIRAQAVLDDGTLVHD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHH
FLFKETERMLHVCNAPSPAATSSIPIGRMIAQKLLGINLQAEKDISVTGE
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]