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The map label for this gene is mdtE [H]

Identifier: 225686674

GI number: 225686674

Start: 878454

End: 879560

Strand: Reverse

Name: mdtE [H]

Synonym: BMEA_B0897

Alternate gene names: 225686674

Gene position: 879560-878454 (Counterclockwise)

Preceding gene: 225686675

Following gene: 225686673

Centisome position: 74.19

GC content: 62.15

Gene sequence:

>1107_bases
ATGAAAATCCATATGAACCCCATGTTCCGTGCTGCCGGTTGCGCGGTCCTGTTTGGCGCGCTGACGGTGCTTGCCGCTTG
CGGCAAAGAGGATGAAAAGGCGCAGGAAGCTGCGCCCATCCGCCCGGTTCTCTATACGATCGTGGAGCCGAAGCCTGTCG
CCCAGACGGGTTTTGCGGGCACAGTCGAGCCGCGCTATTCGACCGATCTGGCTTTCCGCGTTCTGGGGCGGGTCATAGCC
CGGCCTGTGAAGGTGGGCGATCTCGTGAAGAAGGGCGAGATGGTGGCAATGCTCGATCCATCGGCGCTTGATCTTGCGGT
GCAATCGGCAAGGGCCGACCTTGCCAGTGCCGAGGCACAATATGCCAATGCTTCGGCAAGCGAGGAACGCCAGCGTATCC
TGCTGCGTGGCAACAATGTTTCGCAGGCCGATTATGACGCGGCCAGGCAGGCGCGCGACAGCGCCGAGGCAGGGCTGGAA
AAGGCGCAGGCCGGTTTGCGCAAGGCCGAAGACCAGCGGGGATATGCCGTGCTGCGTCCCGATTTCGATGGCGTGATCTC
CGCAACGGCTGCCGAAGTCGGGCAGGTGGTTTCGGCGGGTCAGGCGATCGTGACCGTGGCGCGTCCCGATGTCCGGGAAG
CGGTGGTGGATATTCCCGACAGCATGACCGAATATTTCAAGCCCGGCACTCCTTTCAATGTGCAGCTTCAGGCCGATCCT
TCCGTGAAAGGAACGGGAAGCGTGCGCGAGGTCGCGCCGCAGGCCGATGCACAAACGCGCACACGCCGGGTGCGCATCGC
ACTTGACAATCCGCCCGAAGGGTTCCGCCTCGGCGCAACCATTCAGGCCGCTCGTTCCACGCCGGAAACGGGCTTCGTTT
CCCTGCCCATGCCGTCGCTTCTCGAACAGGACGGCAAGACCAGTATATGGGTGATCGATCCCGGAACACAGACCGTGAGC
CTTCACGATGTGCGCGTTATCGAGCGCAAGGGAGCAAGTTTTGTGGCAGGCGGGGTCGAGGCCGGCAGCTATGTGGCAAT
TGCCGGTGTGCATGAACTGAAAGCAGGGCAGAAAGTCCGTCTGGACGAGAAAGGAAGCGGGCTGTGA

Upstream 100 bases:

>100_bases
GCCTTAATAACGGTGAGCTTATCGTGACCAAAGGTGCGCAGATGCTGCGCTCCGGCGAGCCGGTAGAGATCATGCAGGGG
CCTGAAGGAGCTGGCTCGCA

Downstream 100 bases:

>100_bases
AAAAGGGATTCAATCTGTCTGACTGGGCTTTGAATCATCGCTCCCTGGTCTGGTATTTCATGCTCGTTTTTCTCGTTGCG
GGGCTTGTGTCCTATCTGGA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKIHMNPMFRAAGCAVLFGALTVLAACGKEDEKAQEAAPIRPVLYTIVEPKPVAQTGFAGTVEPRYSTDLAFRVLGRVIA
RPVKVGDLVKKGEMVAMLDPSALDLAVQSARADLASAEAQYANASASEERQRILLRGNNVSQADYDAARQARDSAEAGLE
KAQAGLRKAEDQRGYAVLRPDFDGVISATAAEVGQVVSAGQAIVTVARPDVREAVVDIPDSMTEYFKPGTPFNVQLQADP
SVKGTGSVREVAPQADAQTRTRRVRIALDNPPEGFRLGATIQAARSTPETGFVSLPMPSLLEQDGKTSIWVIDPGTQTVS
LHDVRVIERKGASFVAGGVEAGSYVAIAGVHELKAGQKVRLDEKGSGL

Sequences:

>Translated_368_residues
MKIHMNPMFRAAGCAVLFGALTVLAACGKEDEKAQEAAPIRPVLYTIVEPKPVAQTGFAGTVEPRYSTDLAFRVLGRVIA
RPVKVGDLVKKGEMVAMLDPSALDLAVQSARADLASAEAQYANASASEERQRILLRGNNVSQADYDAARQARDSAEAGLE
KAQAGLRKAEDQRGYAVLRPDFDGVISATAAEVGQVVSAGQAIVTVARPDVREAVVDIPDSMTEYFKPGTPFNVQLQADP
SVKGTGSVREVAPQADAQTRTRRVRIALDNPPEGFRLGATIQAARSTPETGFVSLPMPSLLEQDGKTSIWVIDPGTQTVS
LHDVRVIERKGASFVAGGVEAGSYVAIAGVHELKAGQKVRLDEKGSGL
>Mature_368_residues
MKIHMNPMFRAAGCAVLFGALTVLAACGKEDEKAQEAAPIRPVLYTIVEPKPVAQTGFAGTVEPRYSTDLAFRVLGRVIA
RPVKVGDLVKKGEMVAMLDPSALDLAVQSARADLASAEAQYANASASEERQRILLRGNNVSQADYDAARQARDSAEAGLE
KAQAGLRKAEDQRGYAVLRPDFDGVISATAAEVGQVVSAGQAIVTVARPDVREAVVDIPDSMTEYFKPGTPFNVQLQADP
SVKGTGSVREVAPQADAQTRTRRVRIALDNPPEGFRLGATIQAARSTPETGFVSLPMPSLLEQDGKTSIWVIDPGTQTVS
LHDVRVIERKGASFVAGGVEAGSYVAIAGVHELKAGQKVRLDEKGSGL

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=367, Percent_Identity=27.2479564032698, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082036, Length=342, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI87081796, Length=289, Percent_Identity=24.9134948096886, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39002; Mature: 39002

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIHMNPMFRAAGCAVLFGALTVLAACGKEDEKAQEAAPIRPVLYTIVEPKPVAQTGFAG
CEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCC
TVEPRYSTDLAFRVLGRVIARPVKVGDLVKKGEMVAMLDPSALDLAVQSARADLASAEAQ
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
YANASASEERQRILLRGNNVSQADYDAARQARDSAEAGLEKAQAGLRKAEDQRGYAVLRP
HCCCCCCHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
DFDGVISATAAEVGQVVSAGQAIVTVARPDVREAVVDIPDSMTEYFKPGTPFNVQLQADP
CCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCHHHHHHCCCCCCEEEEEEECC
SVKGTGSVREVAPQADAQTRTRRVRIALDNPPEGFRLGATIQAARSTPETGFVSLPMPSL
CCCCCCCHHHHCCCCCCCCCEEEEEEEECCCCCCEEECCEEEECCCCCCCCEEECCCHHH
LEQDGKTSIWVIDPGTQTVSLHDVRVIERKGASFVAGGVEAGSYVAIAGVHELKAGQKVR
HHCCCCEEEEEECCCCCEEEEEHEEEEHHCCCEEEECCCCCCCEEEEECHHHHCCCCEEE
LDEKGSGL
ECCCCCCC
>Mature Secondary Structure
MKIHMNPMFRAAGCAVLFGALTVLAACGKEDEKAQEAAPIRPVLYTIVEPKPVAQTGFAG
CEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCC
TVEPRYSTDLAFRVLGRVIARPVKVGDLVKKGEMVAMLDPSALDLAVQSARADLASAEAQ
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
YANASASEERQRILLRGNNVSQADYDAARQARDSAEAGLEKAQAGLRKAEDQRGYAVLRP
HCCCCCCHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
DFDGVISATAAEVGQVVSAGQAIVTVARPDVREAVVDIPDSMTEYFKPGTPFNVQLQADP
CCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCHHHHHHCCCCCCEEEEEEECC
SVKGTGSVREVAPQADAQTRTRRVRIALDNPPEGFRLGATIQAARSTPETGFVSLPMPSL
CCCCCCCHHHHCCCCCCCCCEEEEEEEECCCCCCEEECCEEEECCCCCCCCEEECCCHHH
LEQDGKTSIWVIDPGTQTVSLHDVRVIERKGASFVAGGVEAGSYVAIAGVHELKAGQKVR
HHCCCCEEEEEECCCCCEEEEEHEEEEHHCCCEEEECCCCCCCEEEEECHHHHCCCCEEE
LDEKGSGL
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]