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The map label for this gene is nemA [H]

Identifier: 225686663

GI number: 225686663

Start: 861474

End: 862589

Strand: Reverse

Name: nemA [H]

Synonym: BMEA_B0883

Alternate gene names: 225686663

Gene position: 862589-861474 (Counterclockwise)

Preceding gene: 225686664

Following gene: 225686661

Centisome position: 72.76

GC content: 58.96

Gene sequence:

>1116_bases
ATGGCCTCATTGTTCGACCCCATTACCATCGGTGATCTGAAGCTTGCAAACCGTATCGTCATGGCGCCGCTGACGCGCAA
CCGCTCGCCGCACGCCGTGCCCAATGATTTGAACGTCATCTATTACGAACAGCGCGCCAGTGCTGGTCTTATCATCACCG
AGGCAACGCCGATCAGCCAACAGGGACAGGGCTATGCGGATGTTCCGGGCCTTTATTCGGCGGAACAACTTGCCGGATGG
CGGCGGGTGAGCGATGCCGTGCATAATGCGGGCGGCAAGATCGTCGCGCAGATGTGGCATGTGGGCCGTATCTCGCATAA
TTCCCTGCAACCGAACGGCGAGAAGCCGGTCGCCCCGTCAGCGATCACGGCCAAGTCGAAGACCTATCTGGTCCACCCGG
ACGGCGCTGGCGAATTCGTGCCGACTTCCGAACCGCGTGCGCTGGAAAAGAGCGAGCTTCCGGGGATCGTTGCAGACTAT
GCCAGGGCGGCCAAGGATGCGGTTGAGACTGCCGGATTTGATGGCGTGGAGATTCACGCGGCCAATGGCTATCTGATCGA
CCAGTTCCTGCGTGGGGGCAGCAATCAGCGCACCGATGAATATGGCGGCTCAATCGAAAATCGCGCGCGCTTTCTGTTTG
AAGTTGTGGATGCCGTGACGAAGGCCATTGGGGCCGGAAAAGTTGGCATTCGCCTGTCGCCGGTCACGCCCGCCAATGAT
GCTTCCGATCCCGATCCGCAACCGCTTTTCGACTATGTGATCGGGAAACTGGCGGCTTGTGGTCTGGCCTATATCCACAT
CATCGAAGGGGCAACCGGTGGTCCGCGTGATTTCCAGCAGGGGCCGCAGCCTTTTGACTATGCGCGCTTCAAGCAGGTTT
ATCGCGATGCGGGCGGCAAGGCTGCATGGATGGTCAATAATGGCTATGATCGCAAGATGGCGGAGGAAGAAGTCGAGGCG
GGGCGTGCAGACCTCGTGGCTTTCGGCAAGCCGTTCATTGCCAATCCTGATCTGGTGCGCCGCCTGAAGGAAAACGCGCC
GCTGAACGAACTCGACCAGAAACATATGTATGGTGGCGGGGCAAAGGGCTATACGGATTATCCCGTTCTGGCATGA

Upstream 100 bases:

>100_bases
TACAAGCGCGATGAGGAAGCAATAGCCGCTGCCATCAAAGCCCTGTCTGCTCTTTAACCCGTCCTGAAACCGATCTTTCC
CACTTGAAGGAAATGTATCC

Downstream 100 bases:

>100_bases
TGGATTGAGCAAGGGCCGGGGTTTTCCGGCCTTTGTTTTATAGCCGGGCGCGGGTTGAACGTGGTGTGGCCAGCAGCAGG
TTGGTCTCACTGGCCTTGAT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MASLFDPITIGDLKLANRIVMAPLTRNRSPHAVPNDLNVIYYEQRASAGLIITEATPISQQGQGYADVPGLYSAEQLAGW
RRVSDAVHNAGGKIVAQMWHVGRISHNSLQPNGEKPVAPSAITAKSKTYLVHPDGAGEFVPTSEPRALEKSELPGIVADY
ARAAKDAVETAGFDGVEIHAANGYLIDQFLRGGSNQRTDEYGGSIENRARFLFEVVDAVTKAIGAGKVGIRLSPVTPAND
ASDPDPQPLFDYVIGKLAACGLAYIHIIEGATGGPRDFQQGPQPFDYARFKQVYRDAGGKAAWMVNNGYDRKMAEEEVEA
GRADLVAFGKPFIANPDLVRRLKENAPLNELDQKHMYGGGAKGYTDYPVLA

Sequences:

>Translated_371_residues
MASLFDPITIGDLKLANRIVMAPLTRNRSPHAVPNDLNVIYYEQRASAGLIITEATPISQQGQGYADVPGLYSAEQLAGW
RRVSDAVHNAGGKIVAQMWHVGRISHNSLQPNGEKPVAPSAITAKSKTYLVHPDGAGEFVPTSEPRALEKSELPGIVADY
ARAAKDAVETAGFDGVEIHAANGYLIDQFLRGGSNQRTDEYGGSIENRARFLFEVVDAVTKAIGAGKVGIRLSPVTPAND
ASDPDPQPLFDYVIGKLAACGLAYIHIIEGATGGPRDFQQGPQPFDYARFKQVYRDAGGKAAWMVNNGYDRKMAEEEVEA
GRADLVAFGKPFIANPDLVRRLKENAPLNELDQKHMYGGGAKGYTDYPVLA
>Mature_370_residues
ASLFDPITIGDLKLANRIVMAPLTRNRSPHAVPNDLNVIYYEQRASAGLIITEATPISQQGQGYADVPGLYSAEQLAGWR
RVSDAVHNAGGKIVAQMWHVGRISHNSLQPNGEKPVAPSAITAKSKTYLVHPDGAGEFVPTSEPRALEKSELPGIVADYA
RAAKDAVETAGFDGVEIHAANGYLIDQFLRGGSNQRTDEYGGSIENRARFLFEVVDAVTKAIGAGKVGIRLSPVTPANDA
SDPDPQPLFDYVIGKLAACGLAYIHIIEGATGGPRDFQQGPQPFDYARFKQVYRDAGGKAAWMVNNGYDRKMAEEEVEAG
RADLVAFGKPFIANPDLVRRLKENAPLNELDQKHMYGGGAKGYTDYPVLA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=370, Percent_Identity=48.1081081081081, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1789463, Length=346, Percent_Identity=30.0578034682081, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17559802, Length=365, Percent_Identity=27.3972602739726, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=358, Percent_Identity=26.536312849162, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=356, Percent_Identity=26.123595505618, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17565138, Length=381, Percent_Identity=25.4593175853018, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=351, Percent_Identity=25.0712250712251, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540738, Length=222, Percent_Identity=30.1801801801802, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=388, Percent_Identity=38.9175257731959, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6321973, Length=388, Percent_Identity=38.9175257731959, Blast_Score=221, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39990; Mature: 39859

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLFDPITIGDLKLANRIVMAPLTRNRSPHAVPNDLNVIYYEQRASAGLIITEATPISQ
CCCCCCCCEECCHHHHCEEEECHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCC
QGQGYADVPGLYSAEQLAGWRRVSDAVHNAGGKIVAQMWHVGRISHNSLQPNGEKPVAPS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
AITAKSKTYLVHPDGAGEFVPTSEPRALEKSELPGIVADYARAAKDAVETAGFDGVEIHA
EEEECCCEEEECCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEE
ANGYLIDQFLRGGSNQRTDEYGGSIENRARFLFEVVDAVTKAIGAGKVGIRLSPVTPAND
CCCHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
ASDPDPQPLFDYVIGKLAACGLAYIHIIEGATGGPRDFQQGPQPFDYARFKQVYRDAGGK
CCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHCCCCCCHHHHHHHHHHHCCCC
AAWMVNNGYDRKMAEEEVEAGRADLVAFGKPFIANPDLVRRLKENAPLNELDQKHMYGGG
EEEEEECCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCC
AKGYTDYPVLA
CCCCCCCCCCC
>Mature Secondary Structure 
ASLFDPITIGDLKLANRIVMAPLTRNRSPHAVPNDLNVIYYEQRASAGLIITEATPISQ
CCCCCCCEECCHHHHCEEEECHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCC
QGQGYADVPGLYSAEQLAGWRRVSDAVHNAGGKIVAQMWHVGRISHNSLQPNGEKPVAPS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
AITAKSKTYLVHPDGAGEFVPTSEPRALEKSELPGIVADYARAAKDAVETAGFDGVEIHA
EEEECCCEEEECCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEE
ANGYLIDQFLRGGSNQRTDEYGGSIENRARFLFEVVDAVTKAIGAGKVGIRLSPVTPAND
CCCHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
ASDPDPQPLFDYVIGKLAACGLAYIHIIEGATGGPRDFQQGPQPFDYARFKQVYRDAGGK
CCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHCCCCCCHHHHHHHHHHHCCCC
AAWMVNNGYDRKMAEEEVEAGRADLVAFGKPFIANPDLVRRLKENAPLNELDQKHMYGGG
EEEEEECCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCC
AKGYTDYPVLA
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]