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The map label for this gene is leuB [H]

Identifier: 225686652

GI number: 225686652

Start: 853084

End: 854196

Strand: Reverse

Name: leuB [H]

Synonym: BMEA_B0869

Alternate gene names: 225686652

Gene position: 854196-853084 (Counterclockwise)

Preceding gene: 225686653

Following gene: 225686651

Centisome position: 72.05

GC content: 59.84

Gene sequence:

>1113_bases
ATGGCATCCAGAAAACTTCTTCTCCTGCCCGGCGATGGCATCGGTCCCGAGGCCATGGCGGAGGTCCGCAAGGTTATCGC
TTTCCTCAACTCGGACCTCAATCTCGGTTTTGAAACCGAAGAAGGTCTGGTTGGCGGCTGTGCTTACGATGCACACGGCC
AGGCGATTTCCGATGCGGATATGGAAAAGGCGCTTGCGGCCGATGCCGTGCTTTTTGGCGCGGTTGGCGGCCCGAAGTGG
GACAGCGTGCCTTATGAAGTGCGCCCGGAAGCCGGCCTTTTGCGCCTGCGCAAGGATATGCAGCTTTATGCCAATCTGCG
CCCTGCCATCTGCTACCCGGCGCTGGCCCATTCTTCCTCGCTGAAGCCGGAAGTGATCGAAGGTCTCGATATTCTGATTC
TGCGCGAGCTGACGGGCGGCGTTTATTTCGGCGAACCGAAGGAAATCATCGATCTCGGCAACGGCCAGAAGCGTGGCATC
GACACGCAGGTCTACGACACCTACGAAATCGAACGCATCGCCGATGTGGCCTTCGAGCTGGCGCGCACGCGCCGCAACAA
GGTCACGTCGATGGAAAAGCGCAATGTGATGAAGTCCGGCGTTCTGTGGAATCAGGGCGTTACGGCGCGCCACAAGGAAA
AACATGCCGATGTGCAACTGGAACATATGCTGGCTGATGCTGGCGGCATGCAGCTCGTGCGCTGGCCGAAGCAGTTCGAC
GTCATCCTGACCGATAATCTGTTCGGCGACCTGCTGTCGGATGTGGCCGCCATGCTGACCGGCTCGCTCGGCATGCTTCC
CTCCGCTTCGCTGGGTGCGGCGGACAGCAAGACCGGCAAGCGCAAGGCGCTTTACGAGCCGGTGCACGGTTCTGCACCTG
ATATCGCGGGCAAGGGCATTGCCAACCCGATCGCGATGATCGCGTCGCTTGCCATGTGCCTGCGCTATTCTTTCGGTCTG
GTTGCCGAAGCTGACCGCCTGGAAGCTGCCATTGCTGGCGTTCTGGACGACGGTATCCGCACCGCCGATATCTGGTCGGA
AGGCAATACCAAGGTCGGCACCACCGAAATGGGCGATGCCATCCTTGCAAAGTTCAAGGCACTTTCGGCCTGA

Upstream 100 bases:

>100_bases
TGGCTAGCCTCCATTGTCGTTGGTCTGACGCGCATCATTTTCCGAAAATCCGGTTCCTTCCTTTCGGGGATGCGCTCTGA
AAAAACAGAGGCTTGGCATT

Downstream 100 bases:

>100_bases
TCGAAGCAGAAGCCCGGAGCGGTTCCCGCTTTGGGGGAAACGGAAAGCCTGTCATGCAAGCTGCATGGCGGGCTTTTCTT
TTCGCAATAAGCGCCCGATT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MASRKLLLLPGDGIGPEAMAEVRKVIAFLNSDLNLGFETEEGLVGGCAYDAHGQAISDADMEKALAADAVLFGAVGGPKW
DSVPYEVRPEAGLLRLRKDMQLYANLRPAICYPALAHSSSLKPEVIEGLDILILRELTGGVYFGEPKEIIDLGNGQKRGI
DTQVYDTYEIERIADVAFELARTRRNKVTSMEKRNVMKSGVLWNQGVTARHKEKHADVQLEHMLADAGGMQLVRWPKQFD
VILTDNLFGDLLSDVAAMLTGSLGMLPSASLGAADSKTGKRKALYEPVHGSAPDIAGKGIANPIAMIASLAMCLRYSFGL
VAEADRLEAAIAGVLDDGIRTADIWSEGNTKVGTTEMGDAILAKFKALSA

Sequences:

>Translated_370_residues
MASRKLLLLPGDGIGPEAMAEVRKVIAFLNSDLNLGFETEEGLVGGCAYDAHGQAISDADMEKALAADAVLFGAVGGPKW
DSVPYEVRPEAGLLRLRKDMQLYANLRPAICYPALAHSSSLKPEVIEGLDILILRELTGGVYFGEPKEIIDLGNGQKRGI
DTQVYDTYEIERIADVAFELARTRRNKVTSMEKRNVMKSGVLWNQGVTARHKEKHADVQLEHMLADAGGMQLVRWPKQFD
VILTDNLFGDLLSDVAAMLTGSLGMLPSASLGAADSKTGKRKALYEPVHGSAPDIAGKGIANPIAMIASLAMCLRYSFGL
VAEADRLEAAIAGVLDDGIRTADIWSEGNTKVGTTEMGDAILAKFKALSA
>Mature_369_residues
ASRKLLLLPGDGIGPEAMAEVRKVIAFLNSDLNLGFETEEGLVGGCAYDAHGQAISDADMEKALAADAVLFGAVGGPKWD
SVPYEVRPEAGLLRLRKDMQLYANLRPAICYPALAHSSSLKPEVIEGLDILILRELTGGVYFGEPKEIIDLGNGQKRGID
TQVYDTYEIERIADVAFELARTRRNKVTSMEKRNVMKSGVLWNQGVTARHKEKHADVQLEHMLADAGGMQLVRWPKQFDV
ILTDNLFGDLLSDVAAMLTGSLGMLPSASLGAADSKTGKRKALYEPVHGSAPDIAGKGIANPIAMIASLAMCLRYSFGLV
AEADRLEAAIAGVLDDGIRTADIWSEGNTKVGTTEMGDAILAKFKALSA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=338, Percent_Identity=34.0236686390533, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI4758582, Length=358, Percent_Identity=30.4469273743017, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI28178816, Length=321, Percent_Identity=29.595015576324, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI28178821, Length=319, Percent_Identity=29.153605015674, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI28178838, Length=342, Percent_Identity=30.4093567251462, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI28178819, Length=149, Percent_Identity=36.241610738255, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87081683, Length=365, Percent_Identity=50.4109589041096, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1788101, Length=371, Percent_Identity=33.4231805929919, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1787381, Length=308, Percent_Identity=27.2727272727273, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=360, Percent_Identity=30.8333333333333, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17505779, Length=369, Percent_Identity=31.7073170731707, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=346, Percent_Identity=30.0578034682081, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=373, Percent_Identity=30.5630026809651, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=375, Percent_Identity=42.1333333333333, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6322097, Length=364, Percent_Identity=34.0659340659341, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=351, Percent_Identity=30.1994301994302, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324291, Length=363, Percent_Identity=27.5482093663912, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24643270, Length=344, Percent_Identity=33.4302325581395, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24643268, Length=344, Percent_Identity=33.4302325581395, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24661184, Length=372, Percent_Identity=30.6451612903226, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161078635, Length=374, Percent_Identity=28.8770053475936, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI161078633, Length=374, Percent_Identity=28.8770053475936, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24650122, Length=374, Percent_Identity=28.8770053475936, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161078637, Length=374, Percent_Identity=28.8770053475936, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI161078639, Length=372, Percent_Identity=29.0322580645161, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI281362242, Length=357, Percent_Identity=27.7310924369748, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648872, Length=357, Percent_Identity=27.7310924369748, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20130355, Length=353, Percent_Identity=23.7960339943343, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39714; Mature: 39583

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00470 IDH_IMDH ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRKLLLLPGDGIGPEAMAEVRKVIAFLNSDLNLGFETEEGLVGGCAYDAHGQAISDAD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEECCCCCCCCHHH
MEKALAADAVLFGAVGGPKWDSVPYEVRPEAGLLRLRKDMQLYANLRPAICYPALAHSSS
HHHHHHHHHHHEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
LKPEVIEGLDILILRELTGGVYFGEPKEIIDLGNGQKRGIDTQVYDTYEIERIADVAFEL
CCHHHHCCCHHEEEHHHCCCEEECCCHHHHCCCCCCCCCCCCCEECHHHHHHHHHHHHHH
ARTRRNKVTSMEKRNVMKSGVLWNQGVTARHKEKHADVQLEHMLADAGGMQLVRWPKQFD
HHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHCCHHHHHHHHCCCCCEEEECCCCCC
VILTDNLFGDLLSDVAAMLTGSLGMLPSASLGAADSKTGKRKALYEPVHGSAPDIAGKGI
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
ANPIAMIASLAMCLRYSFGLVAEADRLEAAIAGVLDDGIRTADIWSEGNTKVGTTEMGDA
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHH
ILAKFKALSA
HHHHHHHHCC
>Mature Secondary Structure 
ASRKLLLLPGDGIGPEAMAEVRKVIAFLNSDLNLGFETEEGLVGGCAYDAHGQAISDAD
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEECCCCCCCCHHH
MEKALAADAVLFGAVGGPKWDSVPYEVRPEAGLLRLRKDMQLYANLRPAICYPALAHSSS
HHHHHHHHHHHEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
LKPEVIEGLDILILRELTGGVYFGEPKEIIDLGNGQKRGIDTQVYDTYEIERIADVAFEL
CCHHHHCCCHHEEEHHHCCCEEECCCHHHHCCCCCCCCCCCCCEECHHHHHHHHHHHHHH
ARTRRNKVTSMEKRNVMKSGVLWNQGVTARHKEKHADVQLEHMLADAGGMQLVRWPKQFD
HHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHCCHHHHHHHHCCCCCEEEECCCCCC
VILTDNLFGDLLSDVAAMLTGSLGMLPSASLGAADSKTGKRKALYEPVHGSAPDIAGKGI
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
ANPIAMIASLAMCLRYSFGLVAEADRLEAAIAGVLDDGIRTADIWSEGNTKVGTTEMGDA
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHH
ILAKFKALSA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA