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The map label for this gene is ada [H]

Identifier: 225686651

GI number: 225686651

Start: 851891

End: 852943

Strand: Reverse

Name: ada [H]

Synonym: BMEA_B0868

Alternate gene names: 225686651

Gene position: 852943-851891 (Counterclockwise)

Preceding gene: 225686652

Following gene: 225686649

Centisome position: 71.95

GC content: 58.4

Gene sequence:

>1053_bases
ATGTTGTTCACGCTTCCAAACCAAGACAAACTTTATGATGCGCTTGTTGCCCGCGATGCTTCTTATGAGGGGCGGGCTTA
TGTCGGTGTTCTTTCGACCGGGATTTTCTGCCGCCTGACCTGTCCGGCGCGCAAGCCCAAACCGGAAAACTGCCGCTTCT
TTGCATCTGTCGCCGAATGCATGGCCGATGGTTTTCGGCCCTGCAAAAGATGCCACTCCTTGCAGCCCGCAGCCGAAGCG
GAAATTTTGGTGAAGAAACTTCTCAATGCGCTTGAGACCGATCCTGAACGGCGCTGGAGCGAGGAGGATGTGGTGCGCAT
GGGGTTCGACCCTTCGACCGTAAGGCGCAGTTTCCGCCGCCATTTCGGCATGACCTTTCTGGAAATGGCAAGGCAGGAGC
GGCTGCGGCACGGCTTTCAGGTTCTGGCCGGGGGAGGGCGCGTCATCGATGCGCAGGTTGATGCCGGTTTTGAATCACCT
GAAGCCTTCCGAAGTGCCTTTGTGCGTATCGTGGGGCTGCCGCCTGCCAAACTGCGCAAGGAAGGGTTGCTGCGCGCCGA
CTGGTTCAAGACCCCGCTTGGCACCATGATCGCAATTTGCGACGTGCGCAGCCTGCATCTCCTGGAGTTTGCCGACCGCA
AGGCGCTGCCTGCCGAATTGAAAAAGCTTCATGCTGCCTGTCGCGGCGATATCGGTATCGGCCGGTTTGAGACACATGAT
CTGGTGGAGAGGCAGTTGAATGCGTTTTTCGAGGGGCGGTCAGCGGTATTTGATCTGCCTTTGGTGATGCATGGCAGCCC
TTTCACGCAGGATGTGTGGCGAGAATTGCAAAATATCCGGGCGGGCGAAACGCGAAGCTATAGCGAAATCGCGCGGGCTA
TCGAAAAGCCACTCGCCGTGCGGGCCGTTGCGCGCGCCAATGGTGCGAACCAGATCGCTATCGTCATTCCCTGCCATCGG
GTGATTGGCGCGGACGGGGCGTTGACCGGCTATGGCGGCGGCCTCTGGCGCAAGCAGAAGCTTATCGAAATCGAAGCGCA
GTATCGAAGGTAA

Upstream 100 bases:

>100_bases
CGGAAAGCCTGTCATGCAAGCTGCATGGCGGGCTTTTCTTTTCGCAATAAGCGCCCGATTTTAGGCTGCAACATCCGGGT
CAGAGTTTAAGAATGGCGGC

Downstream 100 bases:

>100_bases
AAACCCGGATAAAAACGCCGCTCCGAAGAGCGGCGTTTCTTTTTTGGCGATGGACGATCAATCGACCTTGTTGAGATCGA
CTCCCTTGGTTTCCTTCACG

Product: methylated-DNA-protein-cysteine methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MLFTLPNQDKLYDALVARDASYEGRAYVGVLSTGIFCRLTCPARKPKPENCRFFASVAECMADGFRPCKRCHSLQPAAEA
EILVKKLLNALETDPERRWSEEDVVRMGFDPSTVRRSFRRHFGMTFLEMARQERLRHGFQVLAGGGRVIDAQVDAGFESP
EAFRSAFVRIVGLPPAKLRKEGLLRADWFKTPLGTMIAICDVRSLHLLEFADRKALPAELKKLHAACRGDIGIGRFETHD
LVERQLNAFFEGRSAVFDLPLVMHGSPFTQDVWRELQNIRAGETRSYSEIARAIEKPLAVRAVARANGANQIAIVIPCHR
VIGADGALTGYGGGLWRKQKLIEIEAQYRR

Sequences:

>Translated_350_residues
MLFTLPNQDKLYDALVARDASYEGRAYVGVLSTGIFCRLTCPARKPKPENCRFFASVAECMADGFRPCKRCHSLQPAAEA
EILVKKLLNALETDPERRWSEEDVVRMGFDPSTVRRSFRRHFGMTFLEMARQERLRHGFQVLAGGGRVIDAQVDAGFESP
EAFRSAFVRIVGLPPAKLRKEGLLRADWFKTPLGTMIAICDVRSLHLLEFADRKALPAELKKLHAACRGDIGIGRFETHD
LVERQLNAFFEGRSAVFDLPLVMHGSPFTQDVWRELQNIRAGETRSYSEIARAIEKPLAVRAVARANGANQIAIVIPCHR
VIGADGALTGYGGGLWRKQKLIEIEAQYRR
>Mature_350_residues
MLFTLPNQDKLYDALVARDASYEGRAYVGVLSTGIFCRLTCPARKPKPENCRFFASVAECMADGFRPCKRCHSLQPAAEA
EILVKKLLNALETDPERRWSEEDVVRMGFDPSTVRRSFRRHFGMTFLEMARQERLRHGFQVLAGGGRVIDAQVDAGFESP
EAFRSAFVRIVGLPPAKLRKEGLLRADWFKTPLGTMIAICDVRSLHLLEFADRKALPAELKKLHAACRGDIGIGRFETHD
LVERQLNAFFEGRSAVFDLPLVMHGSPFTQDVWRELQNIRAGETRSYSEIARAIEKPLAVRAVARANGANQIAIVIPCHR
VIGADGALTGYGGGLWRKQKLIEIEAQYRR

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=109, Percent_Identity=35.7798165137615, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1788542, Length=349, Percent_Identity=33.2378223495702, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1787596, Length=161, Percent_Identity=37.2670807453416, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=50, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=50, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=40, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 39299; Mature: 39299

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFTLPNQDKLYDALVARDASYEGRAYVGVLSTGIFCRLTCPARKPKPENCRFFASVAEC
CEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHH
MADGFRPCKRCHSLQPAAEAEILVKKLLNALETDPERRWSEEDVVRMGFDPSTVRRSFRR
HHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCHHEEECCCHHHHHHHHHH
HFGMTFLEMARQERLRHGFQVLAGGGRVIDAQVDAGFESPEAFRSAFVRIVGLPPAKLRK
HHCHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHH
EGLLRADWFKTPLGTMIAICDVRSLHLLEFADRKALPAELKKLHAACRGDIGIGRFETHD
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHH
LVERQLNAFFEGRSAVFDLPLVMHGSPFTQDVWRELQNIRAGETRSYSEIARAIEKPLAV
HHHHHHHHHHCCCCCEEECCEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RAVARANGANQIAIVIPCHRVIGADGALTGYGGGLWRKQKLIEIEAQYRR
HHHHHCCCCCEEEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLFTLPNQDKLYDALVARDASYEGRAYVGVLSTGIFCRLTCPARKPKPENCRFFASVAEC
CEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHH
MADGFRPCKRCHSLQPAAEAEILVKKLLNALETDPERRWSEEDVVRMGFDPSTVRRSFRR
HHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCHHEEECCCHHHHHHHHHH
HFGMTFLEMARQERLRHGFQVLAGGGRVIDAQVDAGFESPEAFRSAFVRIVGLPPAKLRK
HHCHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHH
EGLLRADWFKTPLGTMIAICDVRSLHLLEFADRKALPAELKKLHAACRGDIGIGRFETHD
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHH
LVERQLNAFFEGRSAVFDLPLVMHGSPFTQDVWRELQNIRAGETRSYSEIARAIEKPLAV
HHHHHHHHHHCCCCCEEECCEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RAVARANGANQIAIVIPCHRVIGADGALTGYGGGLWRKQKLIEIEAQYRR
HHHHHCCCCCEEEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]