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The map label for this gene is proP [H]

Identifier: 225686650

GI number: 225686650

Start: 849935

End: 851833

Strand: Direct

Name: proP [H]

Synonym: BMEA_B0867

Alternate gene names: 225686650

Gene position: 849935-851833 (Clockwise)

Preceding gene: 225686648

Following gene: 225686659

Centisome position: 71.69

GC content: 58.87

Gene sequence:

>1899_bases
ATGGCAGTCTCGTCAAGCGCCGCGGCCGGCGCACAAAAAATGACGGGCGAAGAGCGCAAGGTCATCTTTGCGTCCTCGCT
CGGTACCGTCTTTGAATGGTATGACTTCTATCTCTATGGAACGCTGGCGTCCTTTATCGGCGCGGCCTTCTTCAACGAAT
ACCCGGAAGCAACCCGAAACATCTTCGTTCTTCTGGCTTTTGCGGCAGGTTTCCTTGTGCGCCCCTTCGGCGCTCTCGTC
TTCGGACGCATCGGCGACCTCGTCGGCCGTAAATATACATTCCTTGTTACCATCGTCATCATGGGCGGATCGACCTTCCT
GGTCGGCCTTCTGCCCGGCTCCGCCTCGCTCGGCATCTTCGCACCGATCATCCTGATTGCGCTGCGCATGTTGCAGGGGC
TGGCGCTCGGCGGCGAATATGGCGGCGCGGCAACCTATGTGGCCGAACATGCGCCGAACAACCGTCGCGGCTTCTTCACT
TCATGGATCCAGACCACGGCAACGCTCGGCCTGTTCCTGTCGCTGCTCGTGATCCTGGGCGCCCAGACCTTGCTCAGCAA
GGACGCCTTCGCGGCCTGGGGCTGGCGTATTCCGTTCCTCGTATCGGTTCTCCTGCTCGGTATTTCCGTCTGGATCCGCA
TGCAGATGAGCGAATCGCCCGCGTTCAAGCGCATGAAGGAAGAAGGCAAAACCTCCAAGGCCCCGCTTTCGGAAGCTTTC
GGCCAGTGGAAGAATGCCAAGATCGCCCTCATCGCCCTTTTCGGCCTCACCGCCGGTCAGGCCGTTGTCTGGTATTGCGG
ACAGTTTTACGCCCTCTTCTTCCTTCAGAACGTGTTGAAGGTGGAAAACCAGTCGGCCAACATCATGGTGGCAATTGCCC
TCCTGATCGGCACCGGCTTCTTCGTGATTTTTGGCTGGCTTTCGGACAAGATCGGCCGCAAGCCGATCATCATGGCTGGT
CTCATCCTCGCCGCCGTCACCTACTTCCCGCTGTTCAAGGCGCTGACCAATGCCGCCAACCCGGCGCTGGCGCAGGCCGA
ACAGACCGTGAAGGCAACGGTTATGGCAGCACCCGGCGACTGTAACTTCCAGTTCAACGCCACCGGCACGTCCAAGTTCA
CCACCTCCTGCGATGTTGCAACAAGCTTCCTCGCCAAGAGTTCGGTGCCTTACGAAACCGTGCAGACGGCGGCGCCCGGA
ACACCTGCGACCATCACCATCGGCGATACCACGATCCAGTCTTATGACGCGGTTCAGGCCGGTACGGATGCCGCAGCCAA
GCAGGCAGCACTTGCCCATGGCGTGAACCTTGCCTTGCAGCAGGCTGGTTTCCCGCTCGTTCGCGATACAGCGAAAGTGC
CGGATAGCAAGCTCGACGCCTTCGTCGCCGCCAACCCGGAATTGCAACTCAATGCAGATGCCATTCGCGCTGGCGACGCC
AAGACGATGGTTTCCGCTGCTGACCTCGTGAAGCAGAAGCTTCTTACCCAGAGCGAAGCTGAAAGCCTTGGCGTCAGTTC
CGAACAGGCCGTTTATTCCATCCCGAATGCGGGCGCATTCAAGATGGTTGCTGATCCTGCACAGGTGAACTGGGTGCTGA
CCATCGCGATCCTGACTATCCTCGTCATCTACGTGACGATGGTATATGGCCCGATTGCAGCAATCCTCGTCGAGATGTTC
CCAACCCGCATCCGCTATACCGGCATGTCGCTACCTTATCATATCGGCAACGGCTGGTTCGGCGGCCTGCTTCCCGCTGG
CGTGTTCGCGCTCAGCGCGGCCAAGGGCGACATCTATTACGGTCTCTGGTACCCGATCATCATCGCATCCGTAACCTTCG
TGATCGGCATGCTGTTCGTGAAGGAAACCAAGGGAGTCGATCTCAACAAGGTCGATTGA

Upstream 100 bases:

>100_bases
GCCTTGAAGGGAGAGGAACCTGTCGAGGCGCATCAGCGGTCTTCGTAGCCCCGGTGAAAAATGACCGCACCTATATAGCT
CTGTTTGGGAGGACAGATTC

Downstream 100 bases:

>100_bases
TCGTCCATCGCCAAAAAAGAAACGCCGCTCTTCGGAGCGGCGTTTTTATCCGGGTTTTTACCTTCGATACTGCGCTTCGA
TTTCGATAAGCTTCTGCTTG

Product: major facilitator superfamily protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MAVSSSAAAGAQKMTGEERKVIFASSLGTVFEWYDFYLYGTLASFIGAAFFNEYPEATRNIFVLLAFAAGFLVRPFGALV
FGRIGDLVGRKYTFLVTIVIMGGSTFLVGLLPGSASLGIFAPIILIALRMLQGLALGGEYGGAATYVAEHAPNNRRGFFT
SWIQTTATLGLFLSLLVILGAQTLLSKDAFAAWGWRIPFLVSVLLLGISVWIRMQMSESPAFKRMKEEGKTSKAPLSEAF
GQWKNAKIALIALFGLTAGQAVVWYCGQFYALFFLQNVLKVENQSANIMVAIALLIGTGFFVIFGWLSDKIGRKPIIMAG
LILAAVTYFPLFKALTNAANPALAQAEQTVKATVMAAPGDCNFQFNATGTSKFTTSCDVATSFLAKSSVPYETVQTAAPG
TPATITIGDTTIQSYDAVQAGTDAAAKQAALAHGVNLALQQAGFPLVRDTAKVPDSKLDAFVAANPELQLNADAIRAGDA
KTMVSAADLVKQKLLTQSEAESLGVSSEQAVYSIPNAGAFKMVADPAQVNWVLTIAILTILVIYVTMVYGPIAAILVEMF
PTRIRYTGMSLPYHIGNGWFGGLLPAGVFALSAAKGDIYYGLWYPIIIASVTFVIGMLFVKETKGVDLNKVD

Sequences:

>Translated_632_residues
MAVSSSAAAGAQKMTGEERKVIFASSLGTVFEWYDFYLYGTLASFIGAAFFNEYPEATRNIFVLLAFAAGFLVRPFGALV
FGRIGDLVGRKYTFLVTIVIMGGSTFLVGLLPGSASLGIFAPIILIALRMLQGLALGGEYGGAATYVAEHAPNNRRGFFT
SWIQTTATLGLFLSLLVILGAQTLLSKDAFAAWGWRIPFLVSVLLLGISVWIRMQMSESPAFKRMKEEGKTSKAPLSEAF
GQWKNAKIALIALFGLTAGQAVVWYCGQFYALFFLQNVLKVENQSANIMVAIALLIGTGFFVIFGWLSDKIGRKPIIMAG
LILAAVTYFPLFKALTNAANPALAQAEQTVKATVMAAPGDCNFQFNATGTSKFTTSCDVATSFLAKSSVPYETVQTAAPG
TPATITIGDTTIQSYDAVQAGTDAAAKQAALAHGVNLALQQAGFPLVRDTAKVPDSKLDAFVAANPELQLNADAIRAGDA
KTMVSAADLVKQKLLTQSEAESLGVSSEQAVYSIPNAGAFKMVADPAQVNWVLTIAILTILVIYVTMVYGPIAAILVEMF
PTRIRYTGMSLPYHIGNGWFGGLLPAGVFALSAAKGDIYYGLWYPIIIASVTFVIGMLFVKETKGVDLNKVD
>Mature_631_residues
AVSSSAAAGAQKMTGEERKVIFASSLGTVFEWYDFYLYGTLASFIGAAFFNEYPEATRNIFVLLAFAAGFLVRPFGALVF
GRIGDLVGRKYTFLVTIVIMGGSTFLVGLLPGSASLGIFAPIILIALRMLQGLALGGEYGGAATYVAEHAPNNRRGFFTS
WIQTTATLGLFLSLLVILGAQTLLSKDAFAAWGWRIPFLVSVLLLGISVWIRMQMSESPAFKRMKEEGKTSKAPLSEAFG
QWKNAKIALIALFGLTAGQAVVWYCGQFYALFFLQNVLKVENQSANIMVAIALLIGTGFFVIFGWLSDKIGRKPIIMAGL
ILAAVTYFPLFKALTNAANPALAQAEQTVKATVMAAPGDCNFQFNATGTSKFTTSCDVATSFLAKSSVPYETVQTAAPGT
PATITIGDTTIQSYDAVQAGTDAAAKQAALAHGVNLALQQAGFPLVRDTAKVPDSKLDAFVAANPELQLNADAIRAGDAK
TMVSAADLVKQKLLTQSEAESLGVSSEQAVYSIPNAGAFKMVADPAQVNWVLTIAILTILVIYVTMVYGPIAAILVEMFP
TRIRYTGMSLPYHIGNGWFGGLLPAGVFALSAAKGDIYYGLWYPIIIASVTFVIGMLFVKETKGVDLNKVD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=328, Percent_Identity=35.3658536585366, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI1788292, Length=326, Percent_Identity=33.7423312883436, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1788942, Length=342, Percent_Identity=33.3333333333333, Blast_Score=167, Evalue=3e-42,
Organism=Escherichia coli, GI1789941, Length=346, Percent_Identity=34.393063583815, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI87082231, Length=261, Percent_Identity=24.904214559387, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87082404, Length=328, Percent_Identity=25.9146341463415, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 67423; Mature: 67292

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS00107 PROTEIN_KINASE_ATP ; PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSSSAAAGAQKMTGEERKVIFASSLGTVFEWYDFYLYGTLASFIGAAFFNEYPEATRN
CCCCCCHHCCHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IFVLLAFAAGFLVRPFGALVFGRIGDLVGRKYTFLVTIVIMGGSTFLVGLLPGSASLGIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEECCCEEEEEEECCCCCHHHH
APIILIALRMLQGLALGGEYGGAATYVAEHAPNNRRGFFTSWIQTTATLGLFLSLLVILG
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AQTLLSKDAFAAWGWRIPFLVSVLLLGISVWIRMQMSESPAFKRMKEEGKTSKAPLSEAF
HHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCCCCHHHHH
GQWKNAKIALIALFGLTAGQAVVWYCGQFYALFFLQNVLKVENQSANIMVAIALLIGTGF
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
FVIFGWLSDKIGRKPIIMAGLILAAVTYFPLFKALTNAANPALAQAEQTVKATVMAAPGD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCC
CNFQFNATGTSKFTTSCDVATSFLAKSSVPYETVQTAAPGTPATITIGDTTIQSYDAVQA
CCEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCHHHHHHHHHC
GTDAAAKQAALAHGVNLALQQAGFPLVRDTAKVPDSKLDAFVAANPELQLNADAIRAGDA
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHCEEEECCCCEEECHHHHCCCCH
KTMVSAADLVKQKLLTQSEAESLGVSSEQAVYSIPNAGAFKMVADPAQVNWVLTIAILTI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHHH
LVIYVTMVYGPIAAILVEMFPTRIRYTGMSLPYHIGNGWFGGLLPAGVFALSAAKGDIYY
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCCCCHHHHHHHHHHHHCCCCCEEE
GLWYPIIIASVTFVIGMLFVKETKGVDLNKVD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AVSSSAAAGAQKMTGEERKVIFASSLGTVFEWYDFYLYGTLASFIGAAFFNEYPEATRN
CCCCCHHCCHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IFVLLAFAAGFLVRPFGALVFGRIGDLVGRKYTFLVTIVIMGGSTFLVGLLPGSASLGIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEECCCEEEEEEECCCCCHHHH
APIILIALRMLQGLALGGEYGGAATYVAEHAPNNRRGFFTSWIQTTATLGLFLSLLVILG
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AQTLLSKDAFAAWGWRIPFLVSVLLLGISVWIRMQMSESPAFKRMKEEGKTSKAPLSEAF
HHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCCCCHHHHH
GQWKNAKIALIALFGLTAGQAVVWYCGQFYALFFLQNVLKVENQSANIMVAIALLIGTGF
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
FVIFGWLSDKIGRKPIIMAGLILAAVTYFPLFKALTNAANPALAQAEQTVKATVMAAPGD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCC
CNFQFNATGTSKFTTSCDVATSFLAKSSVPYETVQTAAPGTPATITIGDTTIQSYDAVQA
CCEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCHHHHHHHHHC
GTDAAAKQAALAHGVNLALQQAGFPLVRDTAKVPDSKLDAFVAANPELQLNADAIRAGDA
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHCEEEECCCCEEECHHHHCCCCH
KTMVSAADLVKQKLLTQSEAESLGVSSEQAVYSIPNAGAFKMVADPAQVNWVLTIAILTI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHHH
LVIYVTMVYGPIAAILVEMFPTRIRYTGMSLPYHIGNGWFGGLLPAGVFALSAAKGDIYY
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCCCCHHHHHHHHHHHHCCCCCEEE
GLWYPIIIASVTFVIGMLFVKETKGVDLNKVD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]