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The map label for this gene is mcl2 [H]

Identifier: 225686643

GI number: 225686643

Start: 844767

End: 845765

Strand: Reverse

Name: mcl2 [H]

Synonym: BMEA_B0860

Alternate gene names: 225686643

Gene position: 845765-844767 (Counterclockwise)

Preceding gene: 225686647

Following gene: 225686642

Centisome position: 71.34

GC content: 59.96

Gene sequence:

>999_bases
TTGATGGCGCTCAATAAAATGTCCGTCGTCCTGCCGCCGGTCAGCTTTCAAACTATTTCAGGGCTTGGCCCCCAACTATT
CTCGGTGCTTGCGCTGTGGGGCTGTTCGGTGCACAAATCGGGCATGAAAACAATTGTTCGTCCCCGTCGTTCCGTCCTGT
TTGTTCCTGCCGCAAATACGCGGGCATTGGAAAAATCGCTGACGCTTTCCGCCGATTGCGTGATCTATGATCTGGAGGAT
TCGGTTGCGCCGGAGGCGAAGGCTGCGGCCCGCGAGGCACTTGCAGCGCATCTTTCGGCCCATCCGAAAGTTGCTTTCGA
GCGGATCGTGCGGGTCAATGCGGCAGACACCCCATGGGGCAGGGACGATGTTGCGGCTGTGGCGAAAATGGCTGTAAACG
CCGTCCTTTTGCCGAAGGTGGAGCGCCCGCAGGATGTGATCGAGGCCGCCAATCATCTCGACCGGCAGGATGCCGATCCC
GCCATGGGCGTATGGGCCATGATCGAAACACCGCGCGGCATTTTGAATGTCGATGAGATTGCTGTTCTGGGGCATCGCTC
GGCGGTGCGGCTTGCCTGTTTTGTGGTAGGGCCGAACGATATCGCGCGGGAAACCGGGCTTCGCTCACAGCCGGGACGCC
CTTATCTGGCGCCGTGGCTGATGCAGATTGTGCTTGCGGCGCGCGCTGGCGGCATTGCGGTGCTGGATGGCGTTTATAAT
GATTTTCGTGATAGCGCTGGCTTCGAGGCCGAATGTGCGCAGGGCGCGGCCATGGGTTTTGACGGCAAGACGCTGATCCA
TCCGGGCCAGATCGAAGCGGCCAATCGCGCCTTCTCACCGACGGAGGAGGAAGTGGCCCATGCGCGCGCAGTGCGCGAGA
TTTTTGCCCGGCCGGAAAATGCCGGCAAGGGCGTCGTTTCGCTGGACGGGCAAATGGTCGAGCGCCTGCATCTGGAAATG
GCCGAACGGATTCTCGCGAAGGCTGGTATAAATCTGTAA

Upstream 100 bases:

>100_bases
CCTTTCTGAATGATGCGTCAAAAATAGTTTTTGCGCAATCATCGGGCGCGGCATCGCTTCCAGTTAACATGGAAACGCGG
AGCCGTTCTGGCGACTGGTT

Downstream 100 bases:

>100_bases
CGAAGGAAGCAAGAGCAATGAAATTATACCGTTTCATCACCGGGCCGGACGATTCCGCTTTCTGTCATCGTGTGACGGCG
GCGCTGAACAAGGGCTGGCA

Product: citrate lyase subunit beta

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MMALNKMSVVLPPVSFQTISGLGPQLFSVLALWGCSVHKSGMKTIVRPRRSVLFVPAANTRALEKSLTLSADCVIYDLED
SVAPEAKAAAREALAAHLSAHPKVAFERIVRVNAADTPWGRDDVAAVAKMAVNAVLLPKVERPQDVIEAANHLDRQDADP
AMGVWAMIETPRGILNVDEIAVLGHRSAVRLACFVVGPNDIARETGLRSQPGRPYLAPWLMQIVLAARAGGIAVLDGVYN
DFRDSAGFEAECAQGAAMGFDGKTLIHPGQIEAANRAFSPTEEEVAHARAVREIFARPENAGKGVVSLDGQMVERLHLEM
AERILAKAGINL

Sequences:

>Translated_332_residues
MMALNKMSVVLPPVSFQTISGLGPQLFSVLALWGCSVHKSGMKTIVRPRRSVLFVPAANTRALEKSLTLSADCVIYDLED
SVAPEAKAAAREALAAHLSAHPKVAFERIVRVNAADTPWGRDDVAAVAKMAVNAVLLPKVERPQDVIEAANHLDRQDADP
AMGVWAMIETPRGILNVDEIAVLGHRSAVRLACFVVGPNDIARETGLRSQPGRPYLAPWLMQIVLAARAGGIAVLDGVYN
DFRDSAGFEAECAQGAAMGFDGKTLIHPGQIEAANRAFSPTEEEVAHARAVREIFARPENAGKGVVSLDGQMVERLHLEM
AERILAKAGINL
>Mature_332_residues
MMALNKMSVVLPPVSFQTISGLGPQLFSVLALWGCSVHKSGMKTIVRPRRSVLFVPAANTRALEKSLTLSADCVIYDLED
SVAPEAKAAAREALAAHLSAHPKVAFERIVRVNAADTPWGRDDVAAVAKMAVNAVLLPKVERPQDVIEAANHLDRQDADP
AMGVWAMIETPRGILNVDEIAVLGHRSAVRLACFVVGPNDIARETGLRSQPGRPYLAPWLMQIVLAARAGGIAVLDGVYN
DFRDSAGFEAECAQGAAMGFDGKTLIHPGQIEAANRAFSPTEEEVAHARAVREIFARPENAGKGVVSLDGQMVERLHLEM
AERILAKAGINL

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=291, Percent_Identity=31.2714776632302, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI87081764, Length=292, Percent_Identity=38.013698630137, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17557458, Length=286, Percent_Identity=31.8181818181818, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6; 4.1.3.34

Molecular weight: Translated: 35617; Mature: 35617

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMALNKMSVVLPPVSFQTISGLGPQLFSVLALWGCSVHKSGMKTIVRPRRSVLFVPAANT
CCCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCC
RALEKSLTLSADCVIYDLEDSVAPEAKAAAREALAAHLSAHPKVAFERIVRVNAADTPWG
HHHHHHCCEECCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
RDDVAAVAKMAVNAVLLPKVERPQDVIEAANHLDRQDADPAMGVWAMIETPRGILNVDEI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHEEEEEECCCCCCCHHHH
AVLGHRSAVRLACFVVGPNDIARETGLRSQPGRPYLAPWLMQIVLAARAGGIAVLDGVYN
HHHCCCCEEEEEEEEECCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHH
DFRDSAGFEAECAQGAAMGFDGKTLIHPGQIEAANRAFSPTEEEVAHARAVREIFARPEN
HHHHCCCCCHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
AGKGVVSLDGQMVERLHLEMAERILAKAGINL
CCCCEEEECHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMALNKMSVVLPPVSFQTISGLGPQLFSVLALWGCSVHKSGMKTIVRPRRSVLFVPAANT
CCCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCC
RALEKSLTLSADCVIYDLEDSVAPEAKAAAREALAAHLSAHPKVAFERIVRVNAADTPWG
HHHHHHCCEECCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
RDDVAAVAKMAVNAVLLPKVERPQDVIEAANHLDRQDADPAMGVWAMIETPRGILNVDEI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHEEEEEECCCCCCCHHHH
AVLGHRSAVRLACFVVGPNDIARETGLRSQPGRPYLAPWLMQIVLAARAGGIAVLDGVYN
HHHCCCCEEEEEEEEECCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHH
DFRDSAGFEAECAQGAAMGFDGKTLIHPGQIEAANRAFSPTEEEVAHARAVREIFARPEN
HHHHCCCCCHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
AGKGVVSLDGQMVERLHLEMAERILAKAGINL
CCCCEEEECHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA