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The map label for this gene is lacR [H]

Identifier: 225686618

GI number: 225686618

Start: 821721

End: 822542

Strand: Reverse

Name: lacR [H]

Synonym: BMEA_B0835

Alternate gene names: 225686618

Gene position: 822542-821721 (Counterclockwise)

Preceding gene: 225686619

Following gene: 225686617

Centisome position: 69.38

GC content: 58.64

Gene sequence:

>822_bases
GTGCCGAACGACAGCCGGCATGCGCGTCAGCTTGTTCGCCGCCAGCAGATTGCGGAAACTGTTATGGCCGAAGGCTCGAT
GCGGATCGAGGATCTGACCGAGCGTTTTGGCATCAGCCTGATGACCGCGCATCGCGATGTTGATGAACTGGTCAGCCGCG
GCCTGTTCCGCAAATCGCGCGGCATTGTATCGGCTGCGCCGACAAGCCTCATCGAGGCGAGCGATCTATATCGCGTCACC
CGTCAGTCGGAAGAAAAGAAGCTGATCGCCGAAGCCGCGATGCAGTTTGTCGAGCCGGGACAGGCGATTTTCTTCGACGA
TTCCACAACCGTCTTGCAAATGGTGCCGCATCTTCCGGGCAAGGGGCCGCTTACCGTCATCACCAACTCGCTCATCTTGA
TGAACGAAGTGCGAGACATGAAGGATGTGACGCTGCTGGGCCTCGGCGGGCAGCTTTATAACTGGTGCAATGCTTTTGTC
GGCGGCATGACGATCCATGAAATTCGCCGTCTTCGTGCCGATGTGGCCTTCATTTCGATTGCTGCGATCACCGACGATCT
TTTGTTCCACCAGTCGCCTGAAATGGTCGAAACCAAGCGCGCCATGCTGGACTGTGCGGCCAGGCGCATCCTGCTGGCCG
ATCACACCAAGTTCGAGCGCCGCGCCCTGCACAATTTCGGCGCGCTCAGCGATTTCGATGTCGTGATCGTCGATGAAAAA
ACGCCTTTCGCGCATATCGAGCGGATGCAGGCGCAAGGCATCAATGTCGTCGTCGCCCGCGCGTCGGAAAAGGTGGATGT
CATACCCGCCAGGCGGCGCTGA

Upstream 100 bases:

>100_bases
TTCGGAACACGCAAGCAAAGGGGGCAAGACGACGGGCATGGCAGTGAGCAACAAGGCAAGCATTGAAAACCTGATGACGA
ATGGCCTCACAACGGGGCAG

Downstream 100 bases:

>100_bases
GGTTCCGCTCCGGGCGGATTATGTCTGATCTTGCAGCTATCTAGCCTGATCTTTATCAGTGGTATTTTAATTATTGCTAC
TAAATACATATGGTGGCTGA

Product: Lactose phosphotransferase system repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MPNDSRHARQLVRRQQIAETVMAEGSMRIEDLTERFGISLMTAHRDVDELVSRGLFRKSRGIVSAAPTSLIEASDLYRVT
RQSEEKKLIAEAAMQFVEPGQAIFFDDSTTVLQMVPHLPGKGPLTVITNSLILMNEVRDMKDVTLLGLGGQLYNWCNAFV
GGMTIHEIRRLRADVAFISIAAITDDLLFHQSPEMVETKRAMLDCAARRILLADHTKFERRALHNFGALSDFDVVIVDEK
TPFAHIERMQAQGINVVVARASEKVDVIPARRR

Sequences:

>Translated_273_residues
MPNDSRHARQLVRRQQIAETVMAEGSMRIEDLTERFGISLMTAHRDVDELVSRGLFRKSRGIVSAAPTSLIEASDLYRVT
RQSEEKKLIAEAAMQFVEPGQAIFFDDSTTVLQMVPHLPGKGPLTVITNSLILMNEVRDMKDVTLLGLGGQLYNWCNAFV
GGMTIHEIRRLRADVAFISIAAITDDLLFHQSPEMVETKRAMLDCAARRILLADHTKFERRALHNFGALSDFDVVIVDEK
TPFAHIERMQAQGINVVVARASEKVDVIPARRR
>Mature_272_residues
PNDSRHARQLVRRQQIAETVMAEGSMRIEDLTERFGISLMTAHRDVDELVSRGLFRKSRGIVSAAPTSLIEASDLYRVTR
QSEEKKLIAEAAMQFVEPGQAIFFDDSTTVLQMVPHLPGKGPLTVITNSLILMNEVRDMKDVTLLGLGGQLYNWCNAFVG
GMTIHEIRRLRADVAFISIAAITDDLLFHQSPEMVETKRAMLDCAARRILLADHTKFERRALHNFGALSDFDVVIVDEKT
PFAHIERMQAQGINVVVARASEKVDVIPARRR

Specific function: Repressor of the lactose catabolism operon. Galactose-6- phosphate is the inducer [H]

COG id: COG1349

COG function: function code KG; Transcriptional regulators of sugar metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH deoR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI226510968, Length=248, Percent_Identity=29.4354838709677, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789829, Length=237, Percent_Identity=29.957805907173, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1789519, Length=251, Percent_Identity=25.4980079681275, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789059, Length=256, Percent_Identity=27.734375, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI87082344, Length=247, Percent_Identity=27.5303643724696, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790635, Length=254, Percent_Identity=26.3779527559055, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788069, Length=234, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789170, Length=231, Percent_Identity=25.974025974026, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787540, Length=240, Percent_Identity=25.4166666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787063, Length=253, Percent_Identity=23.3201581027668, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014036
- InterPro:   IPR001034
- InterPro:   IPR018356
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00455 DeoR; PF08220 HTH_DeoR [H]

EC number: NA

Molecular weight: Translated: 30667; Mature: 30536

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00894 HTH_DEOR_1 ; PS51000 HTH_DEOR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNDSRHARQLVRRQQIAETVMAEGSMRIEDLTERFGISLMTAHRDVDELVSRGLFRKSR
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHC
GIVSAAPTSLIEASDLYRVTRQSEEKKLIAEAAMQFVEPGQAIFFDDSTTVLQMVPHLPG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCC
KGPLTVITNSLILMNEVRDMKDVTLLGLGGQLYNWCNAFVGGMTIHEIRRLRADVAFISI
CCCHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AAITDDLLFHQSPEMVETKRAMLDCAARRILLADHTKFERRALHNFGALSDFDVVIVDEK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEECC
TPFAHIERMQAQGINVVVARASEKVDVIPARRR
CCHHHHHHHHHCCCEEEEEECCCCEECCCCCCC
>Mature Secondary Structure 
PNDSRHARQLVRRQQIAETVMAEGSMRIEDLTERFGISLMTAHRDVDELVSRGLFRKSR
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHC
GIVSAAPTSLIEASDLYRVTRQSEEKKLIAEAAMQFVEPGQAIFFDDSTTVLQMVPHLPG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCC
KGPLTVITNSLILMNEVRDMKDVTLLGLGGQLYNWCNAFVGGMTIHEIRRLRADVAFISI
CCCHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AAITDDLLFHQSPEMVETKRAMLDCAARRILLADHTKFERRALHNFGALSDFDVVIVDEK
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEECC
TPFAHIERMQAQGINVVVARASEKVDVIPARRR
CCHHHHHHHHHCCCEEEEEECCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120234; 1901863 [H]