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The map label for this gene is putR [H]

Identifier: 225686495

GI number: 225686495

Start: 686472

End: 686966

Strand: Direct

Name: putR [H]

Synonym: BMEA_B0698

Alternate gene names: 225686495

Gene position: 686472-686966 (Clockwise)

Preceding gene: 225686489

Following gene: 225686499

Centisome position: 57.9

GC content: 55.76

Gene sequence:

>495_bases
ATGCGAAAGATAGACTCGCTAGACGATATGGATCAGTTCGACCGGCGCATTCTGGAGGTCCTGAGCGAAGATGGGCGCAT
CCCGGTGACGGAACTGTCGAAAAGGGTCGGCCTTTCCAAGACGCCATGTCAGGCGCGACTGAAAAGACTGGTCGATGAAG
GCTATATTCTGGGTTTTCGCGCAGCCATCGACCCGGAAAAACTGGGCCTCGACCATATCGCTTTCACAACGGTCAAATTA
TCGGATACGCGGGAGGCAGCACTTGAAGCGTTCAATGTGGCGGTGCGCAAGATACGCGAGGTAGAGGAATGCCACATGAT
CGCCAGCTCCTTCGACTATCTCCTGAAAGTGCGCACACCCAACATCCGGCGCTACCGCGAGGTGCTGGGCGAAAAAATCT
CCAGCCTGCCCAATGTGGCAAGCACATCCACCTTCGTGGTGATGGAATCCGTAAAGGATAACCGCCCGATCAGGGTCTAT
CCGGGCGCGTTGTGA

Upstream 100 bases:

>100_bases
TTAGTTGCACATTATCATCCGCATAAATTCCATTCTGCCCTCTTTTTAGCTATAAATAGGCAATCCGATCTTATTATTAC
CTCCTATGAAGGCTTATAGA

Downstream 100 bases:

>100_bases
TTGTTTTTATAAGAGTTTGCGGCAAGCAGTCCGAAAACATTTCCATTCGTCACGACAGCCCAGAGCATTTTCGAGCCAAA
AGTGCTAAAAACAAAGAGAT

Product: proline dehydrogenase transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MRKIDSLDDMDQFDRRILEVLSEDGRIPVTELSKRVGLSKTPCQARLKRLVDEGYILGFRAAIDPEKLGLDHIAFTTVKL
SDTREAALEAFNVAVRKIREVEECHMIASSFDYLLKVRTPNIRRYREVLGEKISSLPNVASTSTFVVMESVKDNRPIRVY
PGAL

Sequences:

>Translated_164_residues
MRKIDSLDDMDQFDRRILEVLSEDGRIPVTELSKRVGLSKTPCQARLKRLVDEGYILGFRAAIDPEKLGLDHIAFTTVKL
SDTREAALEAFNVAVRKIREVEECHMIASSFDYLLKVRTPNIRRYREVLGEKISSLPNVASTSTFVVMESVKDNRPIRVY
PGAL
>Mature_164_residues
MRKIDSLDDMDQFDRRILEVLSEDGRIPVTELSKRVGLSKTPCQARLKRLVDEGYILGFRAAIDPEKLGLDHIAFTTVKL
SDTREAALEAFNVAVRKIREVEECHMIASSFDYLLKVRTPNIRRYREVLGEKISSLPNVASTSTFVVMESVKDNRPIRVY
PGAL

Specific function: Transcriptional activator of the putA gene in response to proline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH asnC-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787116, Length=146, Percent_Identity=46.5753424657534, Blast_Score=143, Evalue=6e-36,
Organism=Escherichia coli, GI87081742, Length=143, Percent_Identity=32.8671328671329, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790182, Length=146, Percent_Identity=28.0821917808219, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011008
- InterPro:   IPR000485
- InterPro:   IPR019885
- InterPro:   IPR019888
- InterPro:   IPR019887
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01037 AsnC_trans_reg [H]

EC number: NA

Molecular weight: Translated: 18639; Mature: 18639

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00519 HTH_ASNC_1 ; PS50956 HTH_ASNC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIDSLDDMDQFDRRILEVLSEDGRIPVTELSKRVGLSKTPCQARLKRLVDEGYILGFR
CCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEE
AAIDPEKLGLDHIAFTTVKLSDTREAALEAFNVAVRKIREVEECHMIASSFDYLLKVRTP
CCCCHHHHCCCHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
NIRRYREVLGEKISSLPNVASTSTFVVMESVKDNRPIRVYPGAL
CHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCCCCCEEECCCCC
>Mature Secondary Structure
MRKIDSLDDMDQFDRRILEVLSEDGRIPVTELSKRVGLSKTPCQARLKRLVDEGYILGFR
CCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEE
AAIDPEKLGLDHIAFTTVKLSDTREAALEAFNVAVRKIREVEECHMIASSFDYLLKVRTP
CCCCHHHHCCCHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
NIRRYREVLGEKISSLPNVASTSTFVVMESVKDNRPIRVYPGAL
CHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606160 [H]