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The map label for this gene is ilvE [H]

Identifier: 225686444

GI number: 225686444

Start: 626594

End: 627484

Strand: Direct

Name: ilvE [H]

Synonym: BMEA_B0640

Alternate gene names: 225686444

Gene position: 626594-627484 (Clockwise)

Preceding gene: 225686443

Following gene: 225686445

Centisome position: 52.85

GC content: 58.02

Gene sequence:

>891_bases
ATGTACAACGCCAATACTATTGCAAGGAACGAGAGAAATGACCGACACACGGGCAATGTGGACCTATTCAAGGGCGAATG
GCGTGAAGGCGACGTACGCATTCTCGGCGCAGCCTCACAGGCGACCTGGCTCGGCTCTCTCGTATTCGACGGCGCACGGC
TTTTTGAGGGTGTGACGCCCGATCTCGATCGCCATTCGGCGCGCGCCAACGATTCCGCTCGTGCGCTCGGCCTTGAGCCG
ACGCTTTCGGCCAATGATATCGAGGCACTGGCGCGCGAGGGCCTGAAAAAATTTGCACCGGACACGGATGTCTATATCCG
CCCCATGTACTGGGCCGAAGAAGGCGATGCCAGCACCGTTGCGCCGCTTGCCTCCTCCACCGATTTTGCGCTCTGCCTTG
AAGCCATTCCAATGGTAGAGCCGAAGGGTTTCACCATTACCACCACTTCTTTCCGCCGCCCTTATCTTGAAGTCATGCCG
GTCAACGCCAAGGCCGCCTGCCTCTATCCGAACAATGCCCGCATGTTGCGCGAAGCCAAGGCCAAGGGTTTTCATAATGC
GCTCGTCACCGATGTTCTCGGCAATGTCGCCGAAACGGCGACGTCGAACGTCTTCATGGTGCGCGGCGGCGAAGTCTTCA
CGCCGGTTCCAAATGGCACATTCCTCAACGGCATCACACGCCAGCGCGTTATCAAGCTTTTGCGTGAAGCGGGGGTAAGT
GTTCACGAAACGACGCTCAAGATCGAAGATTTCCGCGAAGCGGACGAGATTTTCTCCACCGGCAACATGAGCAAGGTCGT
GCCGATCATCGGTTTCGACGAGCGCAAACTCGATTATGGCCTGGTGACAAAGCGTGCCCGCGCCCTTTACTGGGAATGGG
CCCACGCCTGA

Upstream 100 bases:

>100_bases
AAAAGCGGGGTATTTCTGAGGCGCAAAATGAAGACAAGTTCCCGCTTGTTTCCGCCCTGCCCCACCCCTACGATACGGCT
GCCTTCCTGACGGAAGCAGC

Downstream 100 bases:

>100_bases
CGGCAATCAACCGCAATCAATCTCCGGGGCAAAACCCGGAGATTTCCTGCAAGGGTTTCCGCTTTAACAAAACCGTCCGA
AGCACTCCGATGATTTATTT

Product: aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MYNANTIARNERNDRHTGNVDLFKGEWREGDVRILGAASQATWLGSLVFDGARLFEGVTPDLDRHSARANDSARALGLEP
TLSANDIEALAREGLKKFAPDTDVYIRPMYWAEEGDASTVAPLASSTDFALCLEAIPMVEPKGFTITTTSFRRPYLEVMP
VNAKAACLYPNNARMLREAKAKGFHNALVTDVLGNVAETATSNVFMVRGGEVFTPVPNGTFLNGITRQRVIKLLREAGVS
VHETTLKIEDFREADEIFSTGNMSKVVPIIGFDERKLDYGLVTKRARALYWEWAHA

Sequences:

>Translated_296_residues
MYNANTIARNERNDRHTGNVDLFKGEWREGDVRILGAASQATWLGSLVFDGARLFEGVTPDLDRHSARANDSARALGLEP
TLSANDIEALAREGLKKFAPDTDVYIRPMYWAEEGDASTVAPLASSTDFALCLEAIPMVEPKGFTITTTSFRRPYLEVMP
VNAKAACLYPNNARMLREAKAKGFHNALVTDVLGNVAETATSNVFMVRGGEVFTPVPNGTFLNGITRQRVIKLLREAGVS
VHETTLKIEDFREADEIFSTGNMSKVVPIIGFDERKLDYGLVTKRARALYWEWAHA
>Mature_296_residues
MYNANTIARNERNDRHTGNVDLFKGEWREGDVRILGAASQATWLGSLVFDGARLFEGVTPDLDRHSARANDSARALGLEP
TLSANDIEALAREGLKKFAPDTDVYIRPMYWAEEGDASTVAPLASSTDFALCLEAIPMVEPKGFTITTTSFRRPYLEVMP
VNAKAACLYPNNARMLREAKAKGFHNALVTDVLGNVAETATSNVFMVRGGEVFTPVPNGTFLNGITRQRVIKLLREAGVS
VHETTLKIEDFREADEIFSTGNMSKVVPIIGFDERKLDYGLVTKRARALYWEWAHA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=286, Percent_Identity=28.3216783216783, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 32757; Mature: 32757

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNANTIARNERNDRHTGNVDLFKGEWREGDVRILGAASQATWLGSLVFDGARLFEGVTP
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCC
DLDRHSARANDSARALGLEPTLSANDIEALAREGLKKFAPDTDVYIRPMYWAEEGDASTV
CCHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEE
APLASSTDFALCLEAIPMVEPKGFTITTTSFRRPYLEVMPVNAKAACLYPNNARMLREAK
CCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECCCCEEEEECCCHHHHHHHH
AKGFHNALVTDVLGNVAETATSNVFMVRGGEVFTPVPNGTFLNGITRQRVIKLLREAGVS
HCCHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHCCCE
VHETTLKIEDFREADEIFSTGNMSKVVPIIGFDERKLDYGLVTKRARALYWEWAHA
EEEEEEEHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHEEEEECCC
>Mature Secondary Structure
MYNANTIARNERNDRHTGNVDLFKGEWREGDVRILGAASQATWLGSLVFDGARLFEGVTP
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCC
DLDRHSARANDSARALGLEPTLSANDIEALAREGLKKFAPDTDVYIRPMYWAEEGDASTV
CCHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEE
APLASSTDFALCLEAIPMVEPKGFTITTTSFRRPYLEVMPVNAKAACLYPNNARMLREAK
CCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECCCCEEEEECCCHHHHHHHH
AKGFHNALVTDVLGNVAETATSNVFMVRGGEVFTPVPNGTFLNGITRQRVIKLLREAGVS
HCCHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHCCCE
VHETTLKIEDFREADEIFSTGNMSKVVPIIGFDERKLDYGLVTKRARALYWEWAHA
EEEEEEEHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]