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The map label for this gene is pcaF [H]

Identifier: 225686416

GI number: 225686416

Start: 596806

End: 598008

Strand: Reverse

Name: pcaF [H]

Synonym: BMEA_B0609

Alternate gene names: 225686416

Gene position: 598008-596806 (Counterclockwise)

Preceding gene: 225686417

Following gene: 225686413

Centisome position: 50.44

GC content: 61.51

Gene sequence:

>1203_bases
ATGACTGAAGCCTATATCTGCGACTATATCCGCACGCCGATCGGCCGTTACGGCGGCGCGCTTTCCTCCGTTCGCGCGGA
TGATCTGGGTGCGGTGCCGCTGAAGGCGCTGATGGAGCGCAATGGCTCGGTCGATTGGGAAGCGGTGGATGACGTGATCT
TCGGTTCCGCCAATCAGGCAGGCGAAGACAATCGCAATGTGGCACGCATGTCGCTGCTGCTGGCAGGCTTGCCGGTTGGC
GTATCCGGCACCACGATCAACCGCCTCTGCGGTTCGGGCATGGATGCCGTCATCACTGCTGCCCGCGCCATCAAGGCAGG
CGAGGCGGAACTGATCGTGGCAGGCGGCGTGGAAAGCATGAGCCGCGCGCCCCTCGTCATGCCGAAGGCTGACAGCGCCT
TCTCCCGCAAGGCGGAAATCTACGACACAACCATTGGCTGGCGCTTCATCAACCCCTTGATGAAAAAGCAGTATGGTGTT
GATTCCATGCCGGAAACGGGCGAAAACGTTGCCGAGGACTACAATATCTCGCGAGAGGATCAGGATGCTTTCGCCTTGCG
CAGCCAGGATAAGGCCGCTGCCGCGCAGGCCAATGGGCGTCTGGCCAGGGAAATCGTTTCCGTCACCATTCCGCAGCGCA
AGGGCGATCCGGTAATCGTCGAGAAGGATGAACATCCGCGCGCAACCAGCCGGGAAGCTTTGGCAAAACTGCCGACGCCG
TTCCGGCAGGGCGGCACGGTCACGGCTGGCAATGCATCCGGTGTCAATGACGGTGCGGCCGCACTTGTCATCGCTTCGGA
AGCGGCGATCAGGAAATATGGTCTGAAACCCATTGCGCGCATTATTGGCGGTGCGGCCGCTGGCGTCCCGCCACGCGTCA
TGGGTATCGGTCCTGCGCCGGCCACCAGGAAGCTTTGCGCCCGCATCGGGATCGCGCCGGATGCTTTTGATGTGATCGAG
TTGAACGAAGCCTTTGCTTCTCAGGGGCTTGCTGTCCTGCGCCAGCTTGGCATCGCCGATGACGACAAGCGCGTCAACCC
GAATGGCGGCGCGATTGCGCTTGGCCATCCGCTCGGCATGTCGGGTGCGCGCATTGCAGGCACGGCTGCACTTGAACTGT
CGCTTAACAATGGCAAATACGCGCTTGCAACCATGTGCATCGGTGTCGGGCAGGGCATTGCCATCGCGCTTGAACGTGCC
TGA

Upstream 100 bases:

>100_bases
CCGCGATGACGCTGGAAGAGCTTCAGGCTATGACCGGCGCCAAGCTGCATATTGAAGGCCCCGTGGCCGATCTTGTCGTT
CCTGAATTGTGAGAAGCATT

Downstream 100 bases:

>100_bases
GTGCACTTGAGTGTGCTTGAAAGCACTTGATACGGCATGAACCAAAACAAAACCCGGATGGCGAGCCATCCGGGTTTTGT
TTGGGCCGGTTTTGCATGAG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTEAYICDYIRTPIGRYGGALSSVRADDLGAVPLKALMERNGSVDWEAVDDVIFGSANQAGEDNRNVARMSLLLAGLPVG
VSGTTINRLCGSGMDAVITAARAIKAGEAELIVAGGVESMSRAPLVMPKADSAFSRKAEIYDTTIGWRFINPLMKKQYGV
DSMPETGENVAEDYNISREDQDAFALRSQDKAAAAQANGRLAREIVSVTIPQRKGDPVIVEKDEHPRATSREALAKLPTP
FRQGGTVTAGNASGVNDGAAALVIASEAAIRKYGLKPIARIIGGAAAGVPPRVMGIGPAPATRKLCARIGIAPDAFDVIE
LNEAFASQGLAVLRQLGIADDDKRVNPNGGAIALGHPLGMSGARIAGTAALELSLNNGKYALATMCIGVGQGIAIALERA

Sequences:

>Translated_400_residues
MTEAYICDYIRTPIGRYGGALSSVRADDLGAVPLKALMERNGSVDWEAVDDVIFGSANQAGEDNRNVARMSLLLAGLPVG
VSGTTINRLCGSGMDAVITAARAIKAGEAELIVAGGVESMSRAPLVMPKADSAFSRKAEIYDTTIGWRFINPLMKKQYGV
DSMPETGENVAEDYNISREDQDAFALRSQDKAAAAQANGRLAREIVSVTIPQRKGDPVIVEKDEHPRATSREALAKLPTP
FRQGGTVTAGNASGVNDGAAALVIASEAAIRKYGLKPIARIIGGAAAGVPPRVMGIGPAPATRKLCARIGIAPDAFDVIE
LNEAFASQGLAVLRQLGIADDDKRVNPNGGAIALGHPLGMSGARIAGTAALELSLNNGKYALATMCIGVGQGIAIALERA
>Mature_399_residues
TEAYICDYIRTPIGRYGGALSSVRADDLGAVPLKALMERNGSVDWEAVDDVIFGSANQAGEDNRNVARMSLLLAGLPVGV
SGTTINRLCGSGMDAVITAARAIKAGEAELIVAGGVESMSRAPLVMPKADSAFSRKAEIYDTTIGWRFINPLMKKQYGVD
SMPETGENVAEDYNISREDQDAFALRSQDKAAAAQANGRLAREIVSVTIPQRKGDPVIVEKDEHPRATSREALAKLPTPF
RQGGTVTAGNASGVNDGAAALVIASEAAIRKYGLKPIARIIGGAAAGVPPRVMGIGPAPATRKLCARIGIAPDAFDVIEL
NEAFASQGLAVLRQLGIADDDKRVNPNGGAIALGHPLGMSGARIAGTAALELSLNNGKYALATMCIGVGQGIAIALERA

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=42.159383033419, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=39.2857142857143, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI4557237, Length=400, Percent_Identity=36, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI4501853, Length=398, Percent_Identity=37.9396984924623, Blast_Score=201, Evalue=7e-52,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=31.3817330210773, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI194353979, Length=395, Percent_Identity=29.1139240506329, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=67.5, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI1788554, Length=402, Percent_Identity=48.2587064676617, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=45.6359102244389, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI48994986, Length=403, Percent_Identity=40.6947890818859, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1788683, Length=424, Percent_Identity=31.1320754716981, Blast_Score=154, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI133906874, Length=397, Percent_Identity=44.3324937027708, Blast_Score=307, Evalue=6e-84,
Organism=Caenorhabditis elegans, GI25147385, Length=401, Percent_Identity=35.4114713216958, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17535921, Length=404, Percent_Identity=35.8910891089109, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17535917, Length=399, Percent_Identity=29.8245614035088, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17551802, Length=436, Percent_Identity=30.045871559633, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=39.0547263681592, Blast_Score=226, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6322031, Length=395, Percent_Identity=35.1898734177215, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24655093, Length=400, Percent_Identity=44.25, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI17648125, Length=401, Percent_Identity=40.1496259351621, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24640423, Length=404, Percent_Identity=36.1386138613861, Blast_Score=228, Evalue=8e-60,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=30.8962264150943, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41603; Mature: 41472

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAYICDYIRTPIGRYGGALSSVRADDLGAVPLKALMERNGSVDWEAVDDVIFGSANQA
CCCHHHHHHHHCCHHHHCCHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
GEDNRNVARMSLLLAGLPVGVSGTTINRLCGSGMDAVITAARAIKAGEAELIVAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCHHHH
SRAPLVMPKADSAFSRKAEIYDTTIGWRFINPLMKKQYGVDSMPETGENVAEDYNISRED
CCCCEEECCCCCHHHCCCHHEECHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCC
QDAFALRSQDKAAAAQANGRLAREIVSVTIPQRKGDPVIVEKDEHPRATSREALAKLPTP
CHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCC
FRQGGTVTAGNASGVNDGAAALVIASEAAIRKYGLKPIARIIGGAAAGVPPRVMGIGPAP
CCCCCEEEECCCCCCCCCCEEEEEECHHHHHHHCCHHHHHHHCCCCCCCCCCEEECCCCC
ATRKLCARIGIAPDAFDVIELNEAFASQGLAVLRQLGIADDDKRVNPNGGAIALGHPLGM
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCC
SGARIAGTAALELSLNNGKYALATMCIGVGQGIAIALERA
CCCEEEEEEEEEEEECCCCEEEHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
TEAYICDYIRTPIGRYGGALSSVRADDLGAVPLKALMERNGSVDWEAVDDVIFGSANQA
CCHHHHHHHHCCHHHHCCHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
GEDNRNVARMSLLLAGLPVGVSGTTINRLCGSGMDAVITAARAIKAGEAELIVAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCHHHH
SRAPLVMPKADSAFSRKAEIYDTTIGWRFINPLMKKQYGVDSMPETGENVAEDYNISRED
CCCCEEECCCCCHHHCCCHHEECHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCC
QDAFALRSQDKAAAAQANGRLAREIVSVTIPQRKGDPVIVEKDEHPRATSREALAKLPTP
CHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCC
FRQGGTVTAGNASGVNDGAAALVIASEAAIRKYGLKPIARIIGGAAAGVPPRVMGIGPAP
CCCCCEEEECCCCCCCCCCEEEEEECHHHHHHHCCHHHHHHHCCCCCCCCCCEEECCCCC
ATRKLCARIGIAPDAFDVIELNEAFASQGLAVLRQLGIADDDKRVNPNGGAIALGHPLGM
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCC
SGARIAGTAALELSLNNGKYALATMCIGVGQGIAIALERA
CCCEEEEEEEEEEEECCCCEEEHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]