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The map label for this gene is pyrB

Identifier: 225686385

GI number: 225686385

Start: 564816

End: 565784

Strand: Direct

Name: pyrB

Synonym: BMEA_B0574

Alternate gene names: 225686385

Gene position: 564816-565784 (Clockwise)

Preceding gene: 225686383

Following gene: 225686386

Centisome position: 47.64

GC content: 60.17

Gene sequence:

>969_bases
ATGACAAATCAAACGGTCTCTCCGCTTTTCCCTCACCGCCACCTGCTTGGCATCAAGGGGCTATCGCCCCTGGACATTCT
CTGCCTTCTCGATCTTGCCGATCAGGAAATCGCTGTCTCCCGGCAGCCGGAGAAGAAAAAGTCCGTGCTGCGCGGTCGCA
CGCAAATCAACCTGTTCTTTGAAGCATCGACACGGACGCAATCTTCGTTCGAGCTGGCCGGAAAACGGCTGGGCGCGGAT
GTGATGAATATGTCCGTCGGCAATTCATCGGTCAAGAAAGGCGAAACGCTCATCGATACGGCGATGACGCTGAACGCCAT
GCAGCCGGATATTCTCGTCATCCGCCATGCTTCCGCCGGTGCTGCCGCCCTTCTCGCGCAGAAAGTGGGTTGCTCGGTGG
TCAATGCGGGCGACGGCGCGCATGAACACCCCACACAGGCGCTTCTCGATGCGCTGACAATCCGCCGCGCCAAGGGCCAG
ATCGAAAATCTGATCGTCGCCATCTGCGGTGACGTTCTGCATTCGCGCGTGGCGCGCTCCAACATTCTCCTGCTCAACGC
GCTCGGCGCGCGGGTGCGGGTGGTTGCGCCTTCAACACTCCTGCCCGCCGGCATGGCCGATATGAGCGTGGAAGTTTTCA
ACTCCATGGAGGAGGGGCTGAAAGACGCTGATGTCGTCATGATGCTGCGCCTCCAGCGCGAGCGCATGGCGGGTTCCTTC
GTGCCTTCCGTGCGGGAATATTTCCATTTCTATGGCCTGGACCGGGAAAAACTCAAATTCGCCAAACCCGATGCACTTGT
GATGCATCCGGGGCCAATGAACCGGGGCGTCGAGATTGCCTCCGACGTTGCGGACGGGCCGCAAAGCGTCATCCAGCAAC
AGGTGGAAATGGGCGTTGCCGTGCGCATGGCCGTGATGGAGGCTCTGCTCGATCCGCGGCGCAACCCAGGCAACGGAGAA
CCGGCATGA

Upstream 100 bases:

>100_bases
TTGTCGTTCATAGGGCGCAGGTTTTTCTTTGTGAATCGCCTGAAGCCATTATAAGGACGGTTGCGGGACCTGCCTCATGG
TCCTATACGGATGACTTGCC

Downstream 100 bases:

>100_bases
GCGCGATCCTGTTTGAAAACGCCCGGATCGTCGATCCATCACGCGGCCTTGATGAAACCGGCAGCCTTCTGATCGAAAAC
GGCAAAATCGTCGCCGCTGG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTNQTVSPLFPHRHLLGIKGLSPLDILCLLDLADQEIAVSRQPEKKKSVLRGRTQINLFFEASTRTQSSFELAGKRLGAD
VMNMSVGNSSVKKGETLIDTAMTLNAMQPDILVIRHASAGAAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRAKGQ
IENLIVAICGDVLHSRVARSNILLLNALGARVRVVAPSTLLPAGMADMSVEVFNSMEEGLKDADVVMMLRLQRERMAGSF
VPSVREYFHFYGLDREKLKFAKPDALVMHPGPMNRGVEIASDVADGPQSVIQQQVEMGVAVRMAVMEALLDPRRNPGNGE
PA

Sequences:

>Translated_322_residues
MTNQTVSPLFPHRHLLGIKGLSPLDILCLLDLADQEIAVSRQPEKKKSVLRGRTQINLFFEASTRTQSSFELAGKRLGAD
VMNMSVGNSSVKKGETLIDTAMTLNAMQPDILVIRHASAGAAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRAKGQ
IENLIVAICGDVLHSRVARSNILLLNALGARVRVVAPSTLLPAGMADMSVEVFNSMEEGLKDADVVMMLRLQRERMAGSF
VPSVREYFHFYGLDREKLKFAKPDALVMHPGPMNRGVEIASDVADGPQSVIQQQVEMGVAVRMAVMEALLDPRRNPGNGE
PA
>Mature_321_residues
TNQTVSPLFPHRHLLGIKGLSPLDILCLLDLADQEIAVSRQPEKKKSVLRGRTQINLFFEASTRTQSSFELAGKRLGADV
MNMSVGNSSVKKGETLIDTAMTLNAMQPDILVIRHASAGAAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRAKGQI
ENLIVAICGDVLHSRVARSNILLLNALGARVRVVAPSTLLPAGMADMSVEVFNSMEEGLKDADVVMMLRLQRERMAGSFV
PSVREYFHFYGLDREKLKFAKPDALVMHPGPMNRGVEIASDVADGPQSVIQQQVEMGVAVRMAVMEALLDPRRNPGNGEP
A

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=36.3636363636364, Blast_Score=173, Evalue=2e-43,
Organism=Escherichia coli, GI2367364, Length=319, Percent_Identity=36.6771159874608, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1790703, Length=197, Percent_Identity=30.4568527918782, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=321, Percent_Identity=34.2679127725857, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6322331, Length=308, Percent_Identity=34.7402597402597, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24642586, Length=278, Percent_Identity=35.6115107913669, Blast_Score=144, Evalue=7e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_BRUA1 (B2SB57)

Other databases:

- EMBL:   CP000888
- RefSeq:   YP_001932540.1
- ProteinModelPortal:   B2SB57
- SMR:   B2SB57
- GeneID:   6326459
- GenomeReviews:   CP000888_GR
- KEGG:   bmc:BAbS19_II05990
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34803; Mature: 34672

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQTVSPLFPHRHLLGIKGLSPLDILCLLDLADQEIAVSRQPEKKKSVLRGRTQINLFF
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHCCCCCHHHHHHHCCCEEEEEEE
EASTRTQSSFELAGKRLGADVMNMSVGNSSVKKGETLIDTAMTLNAMQPDILVIRHASAG
EECCCCCHHHHHHHHHHCCHHHHCCCCCCCHHCCCHHHHHHHHHCCCCCCEEEEEECCCC
AAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRAKGQIENLIVAICGDVLHSRVARS
HHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NILLLNALGARVRVVAPSTLLPAGMADMSVEVFNSMEEGLKDADVVMMLRLQRERMAGSF
CEEEEEECCCEEEEEECCHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
VPSVREYFHFYGLDREKLKFAKPDALVMHPGPMNRGVEIASDVADGPQSVIQQQVEMGVA
CHHHHHHHHHHCCCHHHHHCCCCCEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHH
VRMAVMEALLDPRRNPGNGEPA
HHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TNQTVSPLFPHRHLLGIKGLSPLDILCLLDLADQEIAVSRQPEKKKSVLRGRTQINLFF
CCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHCCCCCHHHHHHHCCCEEEEEEE
EASTRTQSSFELAGKRLGADVMNMSVGNSSVKKGETLIDTAMTLNAMQPDILVIRHASAG
EECCCCCHHHHHHHHHHCCHHHHCCCCCCCHHCCCHHHHHHHHHCCCCCCEEEEEECCCC
AAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRAKGQIENLIVAICGDVLHSRVARS
HHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NILLLNALGARVRVVAPSTLLPAGMADMSVEVFNSMEEGLKDADVVMMLRLQRERMAGSF
CEEEEEECCCEEEEEECCHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
VPSVREYFHFYGLDREKLKFAKPDALVMHPGPMNRGVEIASDVADGPQSVIQQQVEMGVA
CHHHHHHHHHHCCCHHHHHCCCCCEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHH
VRMAVMEALLDPRRNPGNGEPA
HHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA