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The map label for this gene is bkdA1 [H]

Identifier: 225686316

GI number: 225686316

Start: 481982

End: 483214

Strand: Direct

Name: bkdA1 [H]

Synonym: BMEA_B0497

Alternate gene names: 225686316

Gene position: 481982-483214 (Clockwise)

Preceding gene: 225686315

Following gene: 225686317

Centisome position: 40.66

GC content: 61.15

Gene sequence:

>1233_bases
ATGAGCGATGATGTCGTACTTTCACTGCATGTTCCCGAGCCGGAATGGCGCCCGGGTGATGCGCCGGATTTCTCCCACGT
CAAGATTCCCCGTGCAGGCAGCGTGCGCAGGCCCGAAATCGATGAAAAGCCGGAAGCCATGCGCGATATGGCGTTCAGCA
TCATCCGCGTGCTGAACCGGGAAGGTGAGGCGGTTGGCCCATGGGCGGGCACGCTTACCGACGAGGAACTGAAGGACGGG
CTTCGTCACATGATGATGTTGCGTGCCTATGATGCGCGCATGATGATGGCGCAACGTCAGGGCAAAACCTCGTTCTACAT
GCAGCATCTGGGTGAAGAGGCGGTGAGTTGCGCCTTCCGCAAGGCGCTGCGCAAGGGCGACATGAATTTCCCGACCTACC
GCCAGGCGGGCCTTCTCATCGCGGACGACTATCCCCTCGTCACGATGATGAACCAGATATTCTCCAACGAGCAGGACCCG
CTGAAAGGCCGCCAGCTTCCGGTGATGTATTCCTCGAAAGAGCATGGCTTTTTCACCATTTCCGGCAATCTGGCGACGCA
ATATACGCAGGCCGTCGGCTGGGCGATGGCATCGGCCATCAGCCACGACACGAAGATTGCCGCCGCATGGATCGGTGACG
GCTCGACGGCGGAAAGCGATTTCCACGCCGCACTGGTCTTCGCCTCGACCTACAAGGCCCCGGTGGTGATGAACATCGTC
AACAACCAATGGGCTATTTCCACCTTTCAGGGCATTGCGCGCGGCGGTTCCGGCACTTTTGCGGCGCGGGGCCACGGCTT
CGGCATTCCGTCGCTGCGCGTCGATGGCAATGACTATCTGGCCGTCCATGCGGTGGCGAAATGGGCGGTCGAACGCGCGC
GGCGCAATCTCGGCCCGACCATCGTGGAATATGTGACCTATCGCGCCGGCGGGCACTCCACTTCGGACGATCCGTCCGCC
TATCGCCCCAAGGCGGAAAATGACGCATGGCCGCTTGGCGATCCGATCCTGCGGCTCAAGAACCACCTCATTAAGCGCGG
CGTGTGGTCGGAAGAGCGCCACAAGCAGGCGGAAGCCGAGGTGATGGATATTGTGGTGAGTGCGCAGCGCGAGGCGGAAG
CCATCGGCAAGCTGCATGATGGCCGTAAGCCCTCCATGCGCGACATGTTCGAGGATGTCTATGCCGAAACGCCGCCGCAT
CTTATCCGCCAGCGTCAGGAGGCGGGGTTTTGA

Upstream 100 bases:

>100_bases
GGCTGCAAAGGTTTGCTGTGCAGGCACGGTCGTGATTTTGCGTTTGCCGCCTATAATCTCTCATTCGACTATCCATGCCA
GTTTTGCGAGGTGTGCCCGC

Downstream 100 bases:

>100_bases
GCATTTCCAGGAAAAGTGTGAAGCGCCTACGTAGGGAAACCAGTCCACTGGACTGGTTTCTGATCCTGCTTCGATCCGTT
CGGAAATGCGGAGGAAGTAA

Product: 2-oxoisovalerate dehydrogenase subunit alpha

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSDDVVLSLHVPEPEWRPGDAPDFSHVKIPRAGSVRRPEIDEKPEAMRDMAFSIIRVLNREGEAVGPWAGTLTDEELKDG
LRHMMMLRAYDARMMMAQRQGKTSFYMQHLGEEAVSCAFRKALRKGDMNFPTYRQAGLLIADDYPLVTMMNQIFSNEQDP
LKGRQLPVMYSSKEHGFFTISGNLATQYTQAVGWAMASAISHDTKIAAAWIGDGSTAESDFHAALVFASTYKAPVVMNIV
NNQWAISTFQGIARGGSGTFAARGHGFGIPSLRVDGNDYLAVHAVAKWAVERARRNLGPTIVEYVTYRAGGHSTSDDPSA
YRPKAENDAWPLGDPILRLKNHLIKRGVWSEERHKQAEAEVMDIVVSAQREAEAIGKLHDGRKPSMRDMFEDVYAETPPH
LIRQRQEAGF

Sequences:

>Translated_410_residues
MSDDVVLSLHVPEPEWRPGDAPDFSHVKIPRAGSVRRPEIDEKPEAMRDMAFSIIRVLNREGEAVGPWAGTLTDEELKDG
LRHMMMLRAYDARMMMAQRQGKTSFYMQHLGEEAVSCAFRKALRKGDMNFPTYRQAGLLIADDYPLVTMMNQIFSNEQDP
LKGRQLPVMYSSKEHGFFTISGNLATQYTQAVGWAMASAISHDTKIAAAWIGDGSTAESDFHAALVFASTYKAPVVMNIV
NNQWAISTFQGIARGGSGTFAARGHGFGIPSLRVDGNDYLAVHAVAKWAVERARRNLGPTIVEYVTYRAGGHSTSDDPSA
YRPKAENDAWPLGDPILRLKNHLIKRGVWSEERHKQAEAEVMDIVVSAQREAEAIGKLHDGRKPSMRDMFEDVYAETPPH
LIRQRQEAGF
>Mature_409_residues
SDDVVLSLHVPEPEWRPGDAPDFSHVKIPRAGSVRRPEIDEKPEAMRDMAFSIIRVLNREGEAVGPWAGTLTDEELKDGL
RHMMMLRAYDARMMMAQRQGKTSFYMQHLGEEAVSCAFRKALRKGDMNFPTYRQAGLLIADDYPLVTMMNQIFSNEQDPL
KGRQLPVMYSSKEHGFFTISGNLATQYTQAVGWAMASAISHDTKIAAAWIGDGSTAESDFHAALVFASTYKAPVVMNIVN
NQWAISTFQGIARGGSGTFAARGHGFGIPSLRVDGNDYLAVHAVAKWAVERARRNLGPTIVEYVTYRAGGHSTSDDPSAY
RPKAENDAWPLGDPILRLKNHLIKRGVWSEERHKQAEAEVMDIVVSAQREAEAIGKLHDGRKPSMRDMFEDVYAETPPHL
IRQRQEAGF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=359, Percent_Identity=40.1114206128134, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI258645172, Length=359, Percent_Identity=39.8328690807799, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI291084742, Length=361, Percent_Identity=25.207756232687, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI4505685, Length=362, Percent_Identity=25.414364640884, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI291084744, Length=369, Percent_Identity=24.390243902439, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4885543, Length=300, Percent_Identity=26.3333333333333, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI291084757, Length=188, Percent_Identity=29.2553191489362, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI86563355, Length=360, Percent_Identity=40, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI86563357, Length=360, Percent_Identity=40, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17536047, Length=336, Percent_Identity=25.8928571428571, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32564172, Length=335, Percent_Identity=25.6716417910448, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321026, Length=308, Percent_Identity=28.8961038961039, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21355903, Length=361, Percent_Identity=36.5650969529086, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24639748, Length=302, Percent_Identity=26.4900662251656, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24639746, Length=295, Percent_Identity=23.728813559322, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24639744, Length=293, Percent_Identity=23.8907849829352, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571106, Length=293, Percent_Identity=23.8907849829352, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24639740, Length=294, Percent_Identity=23.8095238095238, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 45719; Mature: 45588

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDVVLSLHVPEPEWRPGDAPDFSHVKIPRAGSVRRPEIDEKPEAMRDMAFSIIRVLNR
CCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
EGEAVGPWAGTLTDEELKDGLRHMMMLRAYDARMMMAQRQGKTSFYMQHLGEEAVSCAFR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KALRKGDMNFPTYRQAGLLIADDYPLVTMMNQIFSNEQDPLKGRQLPVMYSSKEHGFFTI
HHHHHCCCCCCCHHHCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEE
SGNLATQYTQAVGWAMASAISHDTKIAAAWIGDGSTAESDFHAALVFASTYKAPVVMNIV
ECCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHEEEEEECCCCCCEEEEEC
NNQWAISTFQGIARGGSGTFAARGHGFGIPSLRVDGNDYLAVHAVAKWAVERARRNLGPT
CCCCHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHCCCHH
IVEYVTYRAGGHSTSDDPSAYRPKAENDAWPLGDPILRLKNHLIKRGVWSEERHKQAEAE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
VMDIVVSAQREAEAIGKLHDGRKPSMRDMFEDVYAETPPHLIRQRQEAGF
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SDDVVLSLHVPEPEWRPGDAPDFSHVKIPRAGSVRRPEIDEKPEAMRDMAFSIIRVLNR
CCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
EGEAVGPWAGTLTDEELKDGLRHMMMLRAYDARMMMAQRQGKTSFYMQHLGEEAVSCAFR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KALRKGDMNFPTYRQAGLLIADDYPLVTMMNQIFSNEQDPLKGRQLPVMYSSKEHGFFTI
HHHHHCCCCCCCHHHCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEE
SGNLATQYTQAVGWAMASAISHDTKIAAAWIGDGSTAESDFHAALVFASTYKAPVVMNIV
ECCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHEEEEEECCCCCCEEEEEC
NNQWAISTFQGIARGGSGTFAARGHGFGIPSLRVDGNDYLAVHAVAKWAVERARRNLGPT
CCCCHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHCCCHH
IVEYVTYRAGGHSTSDDPSAYRPKAENDAWPLGDPILRLKNHLIKRGVWSEERHKQAEAE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
VMDIVVSAQREAEAIGKLHDGRKPSMRDMFEDVYAETPPHLIRQRQEAGF
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]