Click here to switch to the map view.

The map label for this gene is phaD [H]

Identifier: 225686298

GI number: 225686298

Start: 463608

End: 465236

Strand: Direct

Name: phaD [H]

Synonym: BMEA_B0477

Alternate gene names: 225686298

Gene position: 463608-465236 (Clockwise)

Preceding gene: 225686297

Following gene: 225686299

Centisome position: 39.11

GC content: 59.42

Gene sequence:

>1629_bases
ATGGACTGGAAAACACACCTCGTCGTTGCTCCCATCGTTTTGCCGCTCGTCACGGCGGCGGTGCTGCTGTTGTTCGATGA
GCGCCGGCGCAGGCTGAAAATGGCAATCAGCTTCGTATCCATGGTCTGCCTGATTGCGCTTGCCATCACACTTGTCGGCA
TGGCAAGCGAAAAGGCTCCCGAAGCCATTGTCTATCTGATCGGCAACTGGCCGGCACCGTTCGGCATCGTGCTGGTGCTG
GATCGGCTGGCGGCACTCATGCTCGTGCTGGCCAGCCTGCTCGGTCTGGCCGCACTCAGCTTTTCCTTTGCTTCTTGGCA
CAAGGCGAGCCCGCATTTCCATACGATTTTCCAGCTTCTCCTGATGGGGCTCAACGGGGCTTTTCTCACCGGCGATCTGT
TCAATCTGTTCGTTTTCTTCGAGGTGATGCTGGCAGCATCCTACGGTCTTGCGCTGCATGGTTCGGGCCCGGCGCGCGTC
AAGGCCGGGATGCATTATATCGCGGTCAATCTCGTTGCTTCATCGCTGTTTCTGATCGGTGTCAGCCTGATCTATGGCGT
CACCGGCACACTCAATATGGCAGATCTGGCACAGCGCATTTCGCATGTGGCGGCGCAAGACCGCATGTTGCTGGAAGCTG
GCGCCGCAGTGCTGGGTGTCGCATTTCTCATCAAGGCGGGCATGTGGCCGCTCAATTTCTGGCTTGCACCGGCCTATACC
GCTGCCGCCGCACCTGTTGCAGCGGTGTTTGCGATCATGAGCAAGGTCGGCATCTATGTGCTTTTGCGCCTGTCGCCATT
GATGTTCGGCATCAGCGCCGGTTCATCCTACGGGTTCGGCAATGACTGGCTTTATTTCGGCGGCATGATCACGCTGGCCT
TCGGCCTTATCGGTGTCGTCGCCTCGCAGGCAATGGGCAGGCTTGCCAGCTACAGCATTCTGGTGTCGTCCGGCACACTG
CTTGCCGCCATCGGCAGCGGCAATACGGCAATGACGGGTGCCGCACTTTTCTATATGGTCAGTTCTACGCTCGCCATTGC
TGCCTTTTTCCTGCTGATCGAGCTGGTCGAGCGCGGGCAGGATGCAGCCGCAAACGTTCTTGCCGTTACCATGGAGGCCT
ATGGCGATGACGAGGAGGAAGAAGACGAGGAGGAAGTCGGCACGGTTCTGCCTGCCACGCTCGCCATTCTCGGCACATGT
TTCTGCATTTGCGCTATCCTGCTGATCGGCCTGCCGCCATTCTCCGGCTTCCTCGCCAAGTTCCTCATTCTGACGGCGGT
GTTCAATGGAGATGGCTCTGCCCCCCAGCTCACCGCGCCGGTCAGCGCGGCAAGCTGGGTGCTGGTTTCACTTATCCTGC
TTTCCGGCCTTTCGGCGCTGATAGCCATGACGCGAACCGGCATCCGCACTTTCTGGGCTTCGCTGGAGGGCAATGTGCCG
CGTGTGCTGGTGCGTGAAGTGGTGCCGATTGCCGGTCTGTTGGGTGTGTGCCTCGCCCTCACTATCGCGGCCGGTCCCAC
TATGCGCTATATGGATGAAACAGCCCGCTCGCTGCATAATCCGGATGATTATATCAACAGTGTCCTCAGGGCGCCGCGCA
CTGGCGCAGAAACGGCGGGCGCGCAATGA

Upstream 100 bases:

>100_bases
TCGTCATCGGTTTCGCCATGACGGCGCTTTTCCTTGTCGTGCTGCTTGCCTCGCGCGGGTTGACCCGAACCGACCATGTT
GACGGCAAGGAGAATTGACG

Downstream 100 bases:

>100_bases
AGAAAATCCTTCCCTATCCGCTGCTCTTCGTTTCGCTCGTCCTGTTCTGGCTGCTGCTCAATAGTTTTACCCGCGCGCAG
TTCATCCTTGGTCTGGTGAT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MDWKTHLVVAPIVLPLVTAAVLLLFDERRRRLKMAISFVSMVCLIALAITLVGMASEKAPEAIVYLIGNWPAPFGIVLVL
DRLAALMLVLASLLGLAALSFSFASWHKASPHFHTIFQLLLMGLNGAFLTGDLFNLFVFFEVMLAASYGLALHGSGPARV
KAGMHYIAVNLVASSLFLIGVSLIYGVTGTLNMADLAQRISHVAAQDRMLLEAGAAVLGVAFLIKAGMWPLNFWLAPAYT
AAAAPVAAVFAIMSKVGIYVLLRLSPLMFGISAGSSYGFGNDWLYFGGMITLAFGLIGVVASQAMGRLASYSILVSSGTL
LAAIGSGNTAMTGAALFYMVSSTLAIAAFFLLIELVERGQDAAANVLAVTMEAYGDDEEEEDEEEVGTVLPATLAILGTC
FCICAILLIGLPPFSGFLAKFLILTAVFNGDGSAPQLTAPVSAASWVLVSLILLSGLSALIAMTRTGIRTFWASLEGNVP
RVLVREVVPIAGLLGVCLALTIAAGPTMRYMDETARSLHNPDDYINSVLRAPRTGAETAGAQ

Sequences:

>Translated_542_residues
MDWKTHLVVAPIVLPLVTAAVLLLFDERRRRLKMAISFVSMVCLIALAITLVGMASEKAPEAIVYLIGNWPAPFGIVLVL
DRLAALMLVLASLLGLAALSFSFASWHKASPHFHTIFQLLLMGLNGAFLTGDLFNLFVFFEVMLAASYGLALHGSGPARV
KAGMHYIAVNLVASSLFLIGVSLIYGVTGTLNMADLAQRISHVAAQDRMLLEAGAAVLGVAFLIKAGMWPLNFWLAPAYT
AAAAPVAAVFAIMSKVGIYVLLRLSPLMFGISAGSSYGFGNDWLYFGGMITLAFGLIGVVASQAMGRLASYSILVSSGTL
LAAIGSGNTAMTGAALFYMVSSTLAIAAFFLLIELVERGQDAAANVLAVTMEAYGDDEEEEDEEEVGTVLPATLAILGTC
FCICAILLIGLPPFSGFLAKFLILTAVFNGDGSAPQLTAPVSAASWVLVSLILLSGLSALIAMTRTGIRTFWASLEGNVP
RVLVREVVPIAGLLGVCLALTIAAGPTMRYMDETARSLHNPDDYINSVLRAPRTGAETAGAQ
>Mature_542_residues
MDWKTHLVVAPIVLPLVTAAVLLLFDERRRRLKMAISFVSMVCLIALAITLVGMASEKAPEAIVYLIGNWPAPFGIVLVL
DRLAALMLVLASLLGLAALSFSFASWHKASPHFHTIFQLLLMGLNGAFLTGDLFNLFVFFEVMLAASYGLALHGSGPARV
KAGMHYIAVNLVASSLFLIGVSLIYGVTGTLNMADLAQRISHVAAQDRMLLEAGAAVLGVAFLIKAGMWPLNFWLAPAYT
AAAAPVAAVFAIMSKVGIYVLLRLSPLMFGISAGSSYGFGNDWLYFGGMITLAFGLIGVVASQAMGRLASYSILVSSGTL
LAAIGSGNTAMTGAALFYMVSSTLAIAAFFLLIELVERGQDAAANVLAVTMEAYGDDEEEEDEEEVGTVLPATLAILGTC
FCICAILLIGLPPFSGFLAKFLILTAVFNGDGSAPQLTAPVSAASWVLVSLILLSGLSALIAMTRTGIRTFWASLEGNVP
RVLVREVVPIAGLLGVCLALTIAAGPTMRYMDETARSLHNPDDYINSVLRAPRTGAETAGAQ

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=304, Percent_Identity=30.5921052631579, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788829, Length=451, Percent_Identity=24.390243902439, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 57119; Mature: 57119

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWKTHLVVAPIVLPLVTAAVLLLFDERRRRLKMAISFVSMVCLIALAITLVGMASEKAP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EAIVYLIGNWPAPFGIVLVLDRLAALMLVLASLLGLAALSFSFASWHKASPHFHTIFQLL
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LMGLNGAFLTGDLFNLFVFFEVMLAASYGLALHGSGPARVKAGMHYIAVNLVASSLFLIG
HHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
VSLIYGVTGTLNMADLAQRISHVAAQDRMLLEAGAAVLGVAFLIKAGMWPLNFWLAPAYT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AAAAPVAAVFAIMSKVGIYVLLRLSPLMFGISAGSSYGFGNDWLYFGGMITLAFGLIGVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ASQAMGRLASYSILVSSGTLLAAIGSGNTAMTGAALFYMVSSTLAIAAFFLLIELVERGQ
HHHHHHHHHHHHHEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DAAANVLAVTMEAYGDDEEEEDEEEVGTVLPATLAILGTCFCICAILLIGLPPFSGFLAK
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FLILTAVFNGDGSAPQLTAPVSAASWVLVSLILLSGLSALIAMTRTGIRTFWASLEGNVP
HHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
RVLVREVVPIAGLLGVCLALTIAAGPTMRYMDETARSLHNPDDYINSVLRAPRTGAETAG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
AQ
CC
>Mature Secondary Structure
MDWKTHLVVAPIVLPLVTAAVLLLFDERRRRLKMAISFVSMVCLIALAITLVGMASEKAP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EAIVYLIGNWPAPFGIVLVLDRLAALMLVLASLLGLAALSFSFASWHKASPHFHTIFQLL
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LMGLNGAFLTGDLFNLFVFFEVMLAASYGLALHGSGPARVKAGMHYIAVNLVASSLFLIG
HHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
VSLIYGVTGTLNMADLAQRISHVAAQDRMLLEAGAAVLGVAFLIKAGMWPLNFWLAPAYT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AAAAPVAAVFAIMSKVGIYVLLRLSPLMFGISAGSSYGFGNDWLYFGGMITLAFGLIGVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ASQAMGRLASYSILVSSGTLLAAIGSGNTAMTGAALFYMVSSTLAIAAFFLLIELVERGQ
HHHHHHHHHHHHHEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DAAANVLAVTMEAYGDDEEEEDEEEVGTVLPATLAILGTCFCICAILLIGLPPFSGFLAK
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FLILTAVFNGDGSAPQLTAPVSAASWVLVSLILLSGLSALIAMTRTGIRTFWASLEGNVP
HHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
RVLVREVVPIAGLLGVCLALTIAAGPTMRYMDETARSLHNPDDYINSVLRAPRTGAETAG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
AQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]