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The map label for this gene is miaB [H]

Identifier: 225853579

GI number: 225853579

Start: 2091813

End: 2093216

Strand: Direct

Name: miaB [H]

Synonym: BMEA_A2213

Alternate gene names: 225853579

Gene position: 2091813-2093216 (Clockwise)

Preceding gene: 225853578

Following gene: 225853580

Centisome position: 98.41

GC content: 61.04

Gene sequence:

>1404_bases
ATGAGCGACGACACGACCCAGATTGAACCGGCAATGGCACAGGAAACGAGCCCGCGCGCCAATACGCGCAAGGTTTTCGT
GAAGACCTATGGCTGCCAGATGAATGTCTATGACAGCCAGCGCATGGCCGACAGCCTTGCTGCGGAAGGCTATGTCGCGA
CCGATACGCCGGACGATGCCGATCTGGTGCTGCTCAACACCTGCCATATTCGCGAAAAGGCGTCGGAAAAACTCTATTCC
GCGCTGGGCCGCCTGCGCAAGATGAAGGATGCGCGCGCGGCTGACGGCAAGGAATTGACCATTGGCGTCGCAGGCTGCGT
CGCGCAGGCCGAGGGGCAGGAAATTTTGCGCCGTGCGCCGAATGTCGATCTCGTCATTGGCCCGCAGACATATCACCGCC
TGCCCAATGCGCTGGCGCGGGTGCGTGGTGGCGAGAAGGTGGTGGAAACCGATTACGCCATTGAAGACAAGTTTGAGCAT
CTGCCGGCGCCCAGGCGCGAGGAGACCCGCAAGCGCGGCGTTTCCGCCTTCCTCACCGTGCAGGAAGGCTGCGACAAGTT
CTGCACTTTCTGCGTGGTGCCCTATACGCGCGGCTCGGAAGTTTCGCGCAGCGTGAAGCAGATCGTGGCGGAGGCCGAGC
GCCTTGCCGATAGCGGCGTGCGCGAGCTGACCCTGCTTGGCCAGAACGTCAATGCCTGGCATGGCGAAGGCGAGGATGGC
CGCGAATGGGGCCTTGGCGAATTGCTGTTCCGCCTTGCGCGCATTCCCGGCATTGCGCATCTGCGCTACACAACCAGCCA
CCCGCGCGACATGGATGACAGCCTGATTGCGGCGCATCGCGACCTGCGGCAACTGATGCCCTATCTGCATCTGCCGGTGC
AGTCCGGTTCCGACCGGATCTTGAAGGCGATGAACCGACGCCACAAGGCGGACGAATATCTGCGCCTGATCGAACGTATC
CGTAACGTGCGCCCGGATATGGCGCTGTCGGGCGATTTCATCGTCGGTTTCCCCGGCGAAACCGATCAGGATTTCGAGGA
TACGATGCAGCTTGTGCGTGACGTCAATTATGCGCAGGCCTATTCGTTCAAATATTCGCCGCGCCCTGGAACACCCGGCG
CCGATCTCGACGATCATGTCGAGGAGGCGGTGAAGGACGAGCGCCTCCAGCGCCTTCAGGCGCTCCTGTCGGCACAGCAA
TATGCTTTCCAGGATTCGATGATCGGCCGCAAGATGGATGTGCTTCTGGAGAAGCCCGGCCGCGAGGCGGGCCAGATGGT
GGGCCGCTCGCCATGGCTCCTGCCTGTCATCATCGATGACAACAAGGACCGGGTCGGCGATATTATTCATGTAAAAATCG
TGTCCACGGGCACCAACAGCCTTATTGCGCAAAAACTGGCCTGA

Upstream 100 bases:

>100_bases
TTTGCAAAGCGCGCTTCTGGGGCGCGAAATCCCGGAGGCTTGAAAAGCCGAGCAGATTGTCACCTTCCAAAACGGCAACA
AAAGCTGTAGATAGCCCCGC

Downstream 100 bases:

>100_bases
AGGCATGATGATGCTGAGGCAGAAAATTTCGGCGTCCTGAGTCGTTTGCCGGTAATCCTGTCGAGACTGCGATCGCAAAT
GCGTGAAAGGTTTGTTTTCA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSDDTTQIEPAMAQETSPRANTRKVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDADLVLLNTCHIREKASEKLYS
ALGRLRKMKDARAADGKELTIGVAGCVAQAEGQEILRRAPNVDLVIGPQTYHRLPNALARVRGGEKVVETDYAIEDKFEH
LPAPRREETRKRGVSAFLTVQEGCDKFCTFCVVPYTRGSEVSRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDG
REWGLGELLFRLARIPGIAHLRYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRILKAMNRRHKADEYLRLIERI
RNVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQAYSFKYSPRPGTPGADLDDHVEEAVKDERLQRLQALLSAQQ
YAFQDSMIGRKMDVLLEKPGREAGQMVGRSPWLLPVIIDDNKDRVGDIIHVKIVSTGTNSLIAQKLA

Sequences:

>Translated_467_residues
MSDDTTQIEPAMAQETSPRANTRKVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDADLVLLNTCHIREKASEKLYS
ALGRLRKMKDARAADGKELTIGVAGCVAQAEGQEILRRAPNVDLVIGPQTYHRLPNALARVRGGEKVVETDYAIEDKFEH
LPAPRREETRKRGVSAFLTVQEGCDKFCTFCVVPYTRGSEVSRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDG
REWGLGELLFRLARIPGIAHLRYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRILKAMNRRHKADEYLRLIERI
RNVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQAYSFKYSPRPGTPGADLDDHVEEAVKDERLQRLQALLSAQQ
YAFQDSMIGRKMDVLLEKPGREAGQMVGRSPWLLPVIIDDNKDRVGDIIHVKIVSTGTNSLIAQKLA
>Mature_466_residues
SDDTTQIEPAMAQETSPRANTRKVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDADLVLLNTCHIREKASEKLYSA
LGRLRKMKDARAADGKELTIGVAGCVAQAEGQEILRRAPNVDLVIGPQTYHRLPNALARVRGGEKVVETDYAIEDKFEHL
PAPRREETRKRGVSAFLTVQEGCDKFCTFCVVPYTRGSEVSRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDGR
EWGLGELLFRLARIPGIAHLRYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRILKAMNRRHKADEYLRLIERIR
NVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQAYSFKYSPRPGTPGADLDDHVEEAVKDERLQRLQALLSAQQY
AFQDSMIGRKMDVLLEKPGREAGQMVGRSPWLLPVIIDDNKDRVGDIIHVKIVSTGTNSLIAQKLA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=475, Percent_Identity=33.6842105263158, Blast_Score=272, Evalue=6e-73,
Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=33.6842105263158, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI93277076, Length=466, Percent_Identity=28.1115879828326, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1786882, Length=443, Percent_Identity=48.0812641083521, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1787057, Length=449, Percent_Identity=30.7349665924276, Blast_Score=191, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17553146, Length=468, Percent_Identity=34.1880341880342, Blast_Score=251, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=370, Percent_Identity=28.6486486486486, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=32.9853862212944, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI19922432, Length=471, Percent_Identity=28.6624203821656, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 52291; Mature: 52160

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDTTQIEPAMAQETSPRANTRKVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDA
CCCCCCCCCCHHHHCCCCCCCCCEEEEEEECCEEECCCHHHHHHHHHHCCEEECCCCCCC
DLVLLNTCHIREKASEKLYSALGRLRKMKDARAADGKELTIGVAGCVAQAEGQEILRRAP
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCC
NVDLVIGPQTYHRLPNALARVRGGEKVVETDYAIEDKFEHLPAPRREETRKRGVSAFLTV
CCEEEECCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCHHHHHHCCCCEEEEH
QEGCDKFCTFCVVPYTRGSEVSRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDG
HHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
REWGLGELLFRLARIPGIAHLRYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRI
CCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LKAMNRRHKADEYLRLIERIRNVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQA
HHHHHHHCCHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHCCHHHH
YSFKYSPRPGTPGADLDDHVEEAVKDERLQRLQALLSAQQYAFQDSMIGRKMDVLLEKPG
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
REAGQMVGRSPWLLPVIIDDNKDRVGDIIHVKIVSTGTNSLIAQKLA
HHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCHHHHHHHCC
>Mature Secondary Structure 
SDDTTQIEPAMAQETSPRANTRKVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDA
CCCCCCCCCHHHHCCCCCCCCCEEEEEEECCEEECCCHHHHHHHHHHCCEEECCCCCCC
DLVLLNTCHIREKASEKLYSALGRLRKMKDARAADGKELTIGVAGCVAQAEGQEILRRAP
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCC
NVDLVIGPQTYHRLPNALARVRGGEKVVETDYAIEDKFEHLPAPRREETRKRGVSAFLTV
CCEEEECCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCHHHHHHCCCCEEEEH
QEGCDKFCTFCVVPYTRGSEVSRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDG
HHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
REWGLGELLFRLARIPGIAHLRYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRI
CCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LKAMNRRHKADEYLRLIERIRNVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQA
HHHHHHHCCHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHCCHHHH
YSFKYSPRPGTPGADLDDHVEEAVKDERLQRLQALLSAQQYAFQDSMIGRKMDVLLEKPG
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
REAGQMVGRSPWLLPVIIDDNKDRVGDIIHVKIVSTGTNSLIAQKLA
HHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA