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The map label for this gene is pheS

Identifier: 225853549

GI number: 225853549

Start: 2062643

End: 2063752

Strand: Direct

Name: pheS

Synonym: BMEA_A2182

Alternate gene names: 225853549

Gene position: 2062643-2063752 (Clockwise)

Preceding gene: 225853548

Following gene: 225853550

Centisome position: 97.03

GC content: 59.19

Gene sequence:

>1110_bases
ATGAGCGATCTAGAACAACTTGAACGCCAGATTCTCGAAGACATCGCCGCGGCAGTGGATGAGCAGGGTATTGAGGCCGT
GCGCGTGGCAGCGCTTGGCAAGAAAGGCACGGTTTCGGAAAAGCTGAAGACGCTGGGCGGCATGTCGCCGGAAGAGCGCC
AGATGCAGGGGCCGGCCATCAATGGCCTGAAGAACCGCGTCACCGAGGCCTTGAGCGAGCGCAGGACCGAACTGCGCAAG
GCGGCGGTTGCCGCGCGTCTGGAGCGTGAAAAGGTTGATGTGACGCTCCCGGTGCGTGAAAGCGCGGCATCGCGCGGGCG
CATCCATCCTATTTCGCAGGTGATCGACGAGATCACGGCGATTTTCGCGGATATGGGTTTTTCCATTGCCGAAGGTCCGG
ATATCGAGACCGACTATTATAATTTCACCGCGCTCAACTTCCCCGAAGGCCATCCGGCGCGCGAGATGCACGACACGTTC
TTCTTCAACCCGGATGAAAAGGGCGAGCGCAAGTTGCTGCGCACGCACACTTCGCCGGTGCAGGTGCATACGATGGAGAA
GTTCGCCGCCATGCGCGATAAGGAAGGCCGCGACGAGCCGATTCGCATCGTCATCCCCGGCAAGACCTATCGTATGGATT
CCGACGCCACCCATTCGCCGATGTTCCATCAGGTCGAGGGGCTGGTGGTCGACAAGTCGGCCAATGTCGCCAATATGAAG
TGGGTGCTGGAAGAGTTCTGCAAAGCCTTCTTTGAAGTGCCTTCGGTGAAGATGCGTATGCGCCCGTCCTTCTTCCCGTT
CACCGAGCCGTCTGTTGAAGTCGATATCCAGTGCGACCGCTCCGGCCCGCATGTGAAATTCGGCGAGGGCAATGACTGGC
TGGAAATTCTGGGCTGCGGCATGGTGCATCCCAATGTCTTGCGCATGTCGGGCTATGACCCGGAAGTCTATCAGGGCTTT
GCCTGGGGCATGGGCATCGACCGCATTGCCATGCTGAAATACGGAATGCCGGATCTGCGCGCCTTCTTCGACGCCGATGT
GCGCTGGATCGAGCATTACGGTTTCCGTCCGCTCGACATTCCGACGCTTTTCGGCGGATTGAGCGCGTAA

Upstream 100 bases:

>100_bases
CCAAAGAGAGTGATAATACCAAGAGGCGTGACGTGCCGTAGGCACGGCAGGGATATAATCAAGAGGCGTGGAGTGCCGTA
GGCGCGACAGGATTTAAAGA

Downstream 100 bases:

>100_bases
GGACAGGAAGGAACAGGACAAATGAAATTCACGCTTTCCTGGCTCAAGGATCACCTTGAAACCGATGCGACGCTGGATGA
AATCGTCGAAAAGCTGACCG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSDLEQLERQILEDIAAAVDEQGIEAVRVAALGKKGTVSEKLKTLGGMSPEERQMQGPAINGLKNRVTEALSERRTELRK
AAVAARLEREKVDVTLPVRESAASRGRIHPISQVIDEITAIFADMGFSIAEGPDIETDYYNFTALNFPEGHPAREMHDTF
FFNPDEKGERKLLRTHTSPVQVHTMEKFAAMRDKEGRDEPIRIVIPGKTYRMDSDATHSPMFHQVEGLVVDKSANVANMK
WVLEEFCKAFFEVPSVKMRMRPSFFPFTEPSVEVDIQCDRSGPHVKFGEGNDWLEILGCGMVHPNVLRMSGYDPEVYQGF
AWGMGIDRIAMLKYGMPDLRAFFDADVRWIEHYGFRPLDIPTLFGGLSA

Sequences:

>Translated_369_residues
MSDLEQLERQILEDIAAAVDEQGIEAVRVAALGKKGTVSEKLKTLGGMSPEERQMQGPAINGLKNRVTEALSERRTELRK
AAVAARLEREKVDVTLPVRESAASRGRIHPISQVIDEITAIFADMGFSIAEGPDIETDYYNFTALNFPEGHPAREMHDTF
FFNPDEKGERKLLRTHTSPVQVHTMEKFAAMRDKEGRDEPIRIVIPGKTYRMDSDATHSPMFHQVEGLVVDKSANVANMK
WVLEEFCKAFFEVPSVKMRMRPSFFPFTEPSVEVDIQCDRSGPHVKFGEGNDWLEILGCGMVHPNVLRMSGYDPEVYQGF
AWGMGIDRIAMLKYGMPDLRAFFDADVRWIEHYGFRPLDIPTLFGGLSA
>Mature_368_residues
SDLEQLERQILEDIAAAVDEQGIEAVRVAALGKKGTVSEKLKTLGGMSPEERQMQGPAINGLKNRVTEALSERRTELRKA
AVAARLEREKVDVTLPVRESAASRGRIHPISQVIDEITAIFADMGFSIAEGPDIETDYYNFTALNFPEGHPAREMHDTFF
FNPDEKGERKLLRTHTSPVQVHTMEKFAAMRDKEGRDEPIRIVIPGKTYRMDSDATHSPMFHQVEGLVVDKSANVANMKW
VLEEFCKAFFEVPSVKMRMRPSFFPFTEPSVEVDIQCDRSGPHVKFGEGNDWLEILGCGMVHPNVLRMSGYDPEVYQGFA
WGMGIDRIAMLKYGMPDLRAFFDADVRWIEHYGFRPLDIPTLFGGLSA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=281, Percent_Identity=29.1814946619217, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI5729820, Length=270, Percent_Identity=25.1851851851852, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1788007, Length=353, Percent_Identity=47.0254957507082, Blast_Score=330, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17508957, Length=281, Percent_Identity=29.5373665480427, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=281, Percent_Identity=29.5373665480427, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI32566635, Length=271, Percent_Identity=29.520295202952, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=279, Percent_Identity=27.9569892473118, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6325304, Length=99, Percent_Identity=35.3535353535354, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18858079, Length=273, Percent_Identity=31.1355311355311, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137424, Length=267, Percent_Identity=26.9662921348315, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_BRUA1 (B2S9B8)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935944.1
- ProteinModelPortal:   B2S9B8
- GeneID:   6327092
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I19880
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 41599; Mature: 41468

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLEQLERQILEDIAAAVDEQGIEAVRVAALGKKGTVSEKLKTLGGMSPEERQMQGPAI
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHCCCCH
NGLKNRVTEALSERRTELRKAAVAARLEREKVDVTLPVRESAASRGRIHPISQVIDEITA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHCCCCCCHHHHHHHHHHH
IFADMGFSIAEGPDIETDYYNFTALNFPEGHPAREMHDTFFFNPDEKGERKLLRTHTSPV
HHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHEEECCCCHHHHHHHHHCCCCE
QVHTMEKFAAMRDKEGRDEPIRIVIPGKTYRMDSDATHSPMFHQVEGLVVDKSANVANMK
EEEHHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCHHHHHCCEEEECCCCHHHHH
WVLEEFCKAFFEVPSVKMRMRPSFFPFTEPSVEVDIQCDRSGPHVKFGEGNDWLEILGCG
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCEEEECCCCCHHHHHCCC
MVHPNVLRMSGYDPEVYQGFAWGMGIDRIAMLKYGMPDLRAFFDADVRWIEHYGFRPLDI
CCCCCEEEECCCCHHHHCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCH
PTLFGGLSA
HHHHCCCCC
>Mature Secondary Structure 
SDLEQLERQILEDIAAAVDEQGIEAVRVAALGKKGTVSEKLKTLGGMSPEERQMQGPAI
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHCCCCH
NGLKNRVTEALSERRTELRKAAVAARLEREKVDVTLPVRESAASRGRIHPISQVIDEITA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHCCCCCCHHHHHHHHHHH
IFADMGFSIAEGPDIETDYYNFTALNFPEGHPAREMHDTFFFNPDEKGERKLLRTHTSPV
HHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHEEECCCCHHHHHHHHHCCCCE
QVHTMEKFAAMRDKEGRDEPIRIVIPGKTYRMDSDATHSPMFHQVEGLVVDKSANVANMK
EEEHHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCHHHHHCCEEEECCCCHHHHH
WVLEEFCKAFFEVPSVKMRMRPSFFPFTEPSVEVDIQCDRSGPHVKFGEGNDWLEILGCG
HHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCEEEECCCCCHHHHHCCC
MVHPNVLRMSGYDPEVYQGFAWGMGIDRIAMLKYGMPDLRAFFDADVRWIEHYGFRPLDI
CCCCCEEEECCCCHHHHCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCH
PTLFGGLSA
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA