| Definition | Brucella melitensis ATCC 23457 chromosome chromosome I, complete sequence. |
|---|---|
| Accession | NC_012441 |
| Length | 2,125,701 |
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The map label for this gene is paaF [C]
Identifier: 225853477
GI number: 225853477
Start: 1988906
End: 1989682
Strand: Reverse
Name: paaF [C]
Synonym: BMEA_A2107
Alternate gene names: 225853477
Gene position: 1989682-1988906 (Counterclockwise)
Preceding gene: 225853478
Following gene: 225853476
Centisome position: 93.6
GC content: 61.0
Gene sequence:
>777_bases ATGGCTGACAGAATAGAAATTTCGATCGAAAACCTCATCGCGGTCATGACAATCAGGCGGCCGGAAAAGCTCAATGCGCT CGATATACCGCTCTTGCAGGAACTCTCTACCGCCTGTGATCAGGTCGAGGCGGACAGTAGCGTGCGGGTCGCAATCCTGA CCGGTGAGGGCAAGGCTTTTTCCGCAGGCGGTGACATCAAGGCATGGGGTGGCATGGAGCCGCAAGAATTCGGCCATGCA TGGGTGCGCTATGGGCATCGTGTGTTCGAGCGGCTGGCGACGTTGCGCGTGCCATTGATCGCGGCCATGAACGGCCATGC GCTGGGGGGCGGTCTGGAACTGGCGGGAACCGCCGATATCCGCATTGTTGAGCGACAGGCGAAGATCGGCCTGCCGGAAA CTTCGCTTGGCATGATCCCCGGCTGGTCGGGCACGCAGCGCCTTGTGCGCCGTTTCGGGGCGCAGATCGTGCGCCGCATG GTGCTGGGCGGCGAAATGTTTACGGCAGATGAAGCCCTGTCGCATGGGCTGGTCGATCATGTGGTCGAGACGGGCGAGGC TTTGCAGGCCGCGCTGGCCTATGCGGCGCGGGTGGCCAAACGCGGCCCCGCCGCGCTCGAAATTTCCAAACTGATGCTGT CCGTCGCCAATGGCGAGGACAATGGAACCGCCGTTGAGGCGCTTGGCTCCATTCTTGCCGCCAAGACGGGCGATCTGAAA GAAGGGGTGCGCTCGTTTACCGAAAAGCGCGAAGCGAATTTTGAAGGAAAATGGTAA
Upstream 100 bases:
>100_bases GTCTCGGATCAATTGAAGCTCATGGATGAGGCGCTGTTCCGCGAAGAGCCGATCGGGCTTACGCTGCCGCAAAAGCCTGC GCGAAAGCTGGAGGCGTGAC
Downstream 100 bases:
>100_bases TGAGCACGATCGTTAAGCCACAGCCGTTGACCGGGCTGGAAGTTCGCGAATTCTCCATGCTGATCGATGGAAAATGGGTG GGCAGCGACAGCGGAAAGAG
Product: enoyl-CoA hydratase/isomerase
Products: NA
Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]
Number of amino acids: Translated: 258; Mature: 257
Protein sequence:
>258_residues MADRIEISIENLIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAFSAGGDIKAWGGMEPQEFGHA WVRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADIRIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRM VLGGEMFTADEALSHGLVDHVVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLK EGVRSFTEKREANFEGKW
Sequences:
>Translated_258_residues MADRIEISIENLIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAFSAGGDIKAWGGMEPQEFGHA WVRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADIRIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRM VLGGEMFTADEALSHGLVDHVVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLK EGVRSFTEKREANFEGKW >Mature_257_residues ADRIEISIENLIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAFSAGGDIKAWGGMEPQEFGHAW VRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADIRIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRMV LGGEMFTADEALSHGLVDHVVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLKE GVRSFTEKREANFEGKW
Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]
Homologues:
Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=35.15625, Blast_Score=135, Evalue=3e-32, Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=36.5079365079365, Blast_Score=114, Evalue=8e-26, Organism=Homo sapiens, GI68989263, Length=193, Percent_Identity=33.6787564766839, Blast_Score=106, Evalue=2e-23, Organism=Homo sapiens, GI20127408, Length=183, Percent_Identity=34.4262295081967, Blast_Score=96, Evalue=3e-20, Organism=Homo sapiens, GI31542718, Length=240, Percent_Identity=25.4166666666667, Blast_Score=87, Evalue=2e-17, Organism=Homo sapiens, GI70995211, Length=249, Percent_Identity=28.5140562248996, Blast_Score=86, Evalue=3e-17, Organism=Homo sapiens, GI157694516, Length=216, Percent_Identity=27.3148148148148, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI213417737, Length=216, Percent_Identity=27.3148148148148, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI62530384, Length=208, Percent_Identity=31.25, Blast_Score=82, Evalue=7e-16, Organism=Homo sapiens, GI150378541, Length=253, Percent_Identity=30.0395256916996, Blast_Score=79, Evalue=5e-15, Organism=Homo sapiens, GI157694520, Length=174, Percent_Identity=29.3103448275862, Blast_Score=72, Evalue=4e-13, Organism=Homo sapiens, GI45643119, Length=218, Percent_Identity=25.2293577981651, Blast_Score=71, Evalue=9e-13, Organism=Homo sapiens, GI260275230, Length=218, Percent_Identity=25.2293577981651, Blast_Score=71, Evalue=9e-13, Organism=Homo sapiens, GI260274832, Length=218, Percent_Identity=25.2293577981651, Blast_Score=71, Evalue=1e-12, Organism=Homo sapiens, GI261878539, Length=95, Percent_Identity=41.0526315789474, Blast_Score=70, Evalue=1e-12, Organism=Homo sapiens, GI37594469, Length=186, Percent_Identity=25.2688172043011, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI37594471, Length=186, Percent_Identity=25.2688172043011, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI295842266, Length=206, Percent_Identity=27.6699029126214, Blast_Score=66, Evalue=4e-11, Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=35.1778656126482, Blast_Score=150, Evalue=1e-37, Organism=Escherichia coli, GI1790281, Length=263, Percent_Identity=31.9391634980989, Blast_Score=114, Evalue=7e-27, Organism=Escherichia coli, GI221142681, Length=268, Percent_Identity=33.2089552238806, Blast_Score=105, Evalue=2e-24, Organism=Escherichia coli, GI1788682, Length=197, Percent_Identity=35.0253807106599, Blast_Score=103, Evalue=9e-24, Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=31.5589353612167, Blast_Score=97, Evalue=1e-21, Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=27.843137254902, Blast_Score=88, Evalue=5e-19, Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=28.0155642023346, Blast_Score=81, Evalue=7e-17, Organism=Caenorhabditis elegans, GI25145438, Length=240, Percent_Identity=36.6666666666667, Blast_Score=132, Evalue=2e-31, Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=33.6032388663968, Blast_Score=127, Evalue=6e-30, Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=33.4801762114537, Blast_Score=122, Evalue=1e-28, Organism=Caenorhabditis elegans, GI17558304, Length=175, Percent_Identity=32, Blast_Score=96, Evalue=1e-20, Organism=Caenorhabditis elegans, GI17534483, Length=220, Percent_Identity=25.9090909090909, Blast_Score=92, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17508951, Length=218, Percent_Identity=32.1100917431193, Blast_Score=90, Evalue=1e-18, Organism=Caenorhabditis elegans, GI17508953, Length=218, Percent_Identity=32.1100917431193, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI25144276, Length=218, Percent_Identity=32.1100917431193, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17560910, Length=187, Percent_Identity=31.5508021390374, Blast_Score=88, Evalue=5e-18, Organism=Caenorhabditis elegans, GI17540306, Length=247, Percent_Identity=26.3157894736842, Blast_Score=81, Evalue=6e-16, Organism=Caenorhabditis elegans, GI17549921, Length=215, Percent_Identity=26.5116279069767, Blast_Score=78, Evalue=4e-15, Organism=Caenorhabditis elegans, GI25144157, Length=182, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=5e-14, Organism=Caenorhabditis elegans, GI25144160, Length=182, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=5e-14, Organism=Caenorhabditis elegans, GI25141363, Length=197, Percent_Identity=25.8883248730964, Blast_Score=74, Evalue=7e-14, Organism=Saccharomyces cerevisiae, GI6320241, Length=203, Percent_Identity=26.1083743842365, Blast_Score=72, Evalue=8e-14, Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=36.9477911646586, Blast_Score=135, Evalue=3e-32, Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=36.9477911646586, Blast_Score=135, Evalue=3e-32, Organism=Drosophila melanogaster, GI24653139, Length=268, Percent_Identity=32.089552238806, Blast_Score=126, Evalue=1e-29, Organism=Drosophila melanogaster, GI24583165, Length=215, Percent_Identity=30.2325581395349, Blast_Score=95, Evalue=5e-20, Organism=Drosophila melanogaster, GI19921018, Length=213, Percent_Identity=30.9859154929577, Blast_Score=92, Evalue=4e-19, Organism=Drosophila melanogaster, GI24650670, Length=239, Percent_Identity=30.1255230125523, Blast_Score=87, Evalue=1e-17, Organism=Drosophila melanogaster, GI21357171, Length=195, Percent_Identity=32.3076923076923, Blast_Score=84, Evalue=1e-16, Organism=Drosophila melanogaster, GI45550169, Length=247, Percent_Identity=27.1255060728745, Blast_Score=78, Evalue=5e-15, Organism=Drosophila melanogaster, GI19920382, Length=206, Percent_Identity=27.6699029126214, Blast_Score=75, Evalue=4e-14, Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=24.5210727969349, Blast_Score=71, Evalue=6e-13, Organism=Drosophila melanogaster, GI28571729, Length=172, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=3e-12, Organism=Drosophila melanogaster, GI28571730, Length=172, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=3e-12, Organism=Drosophila melanogaster, GI24583077, Length=193, Percent_Identity=33.160621761658, Blast_Score=69, Evalue=4e-12, Organism=Drosophila melanogaster, GI24583079, Length=193, Percent_Identity=33.160621761658, Blast_Score=69, Evalue=4e-12, Organism=Drosophila melanogaster, GI19921000, Length=193, Percent_Identity=33.160621761658, Blast_Score=69, Evalue=4e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014748 - InterPro: IPR001753 - InterPro: IPR018376 [H]
Pfam domain/function: PF00378 ECH [H]
EC number: =4.2.1.116 [H]
Molecular weight: Translated: 27640; Mature: 27509
Theoretical pI: Translated: 6.05; Mature: 6.05
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 3.1 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 3.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MADRIEISIENLIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAF CCCCEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE SAGGDIKAWGGMEPQEFGHAWVRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADI CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCE RIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRMVLGGEMFTADEALSHGLVDH EEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH VVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLK HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHH EGVRSFTEKREANFEGKW HHHHHHHHHHHCCCCCCC >Mature Secondary Structure ADRIEISIENLIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAF CCCEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE SAGGDIKAWGGMEPQEFGHAWVRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADI CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCE RIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRMVLGGEMFTADEALSHGLVDH EEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH VVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLK HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHH EGVRSFTEKREANFEGKW HHHHHHHHHHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: NA