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The map label for this gene is mdeA [H]

Identifier: 225853401

GI number: 225853401

Start: 1912920

End: 1914203

Strand: Reverse

Name: mdeA [H]

Synonym: BMEA_A2022

Alternate gene names: 225853401

Gene position: 1914203-1912920 (Counterclockwise)

Preceding gene: 225853402

Following gene: 225853400

Centisome position: 90.05

GC content: 58.8

Gene sequence:

>1284_bases
ATGACTGCGCCGCGCCCATCGAAGATCCGTTTGGGCAATCATCTTCTTCATCCAGAAACGCAGATGCTGAATTATGGCTA
CGACTCGGAGCTTTCCGAGGGCGCGGTCAAGCCGCCTGTGTTTCTCACATCGACCTTCGTTTTCAAGACAGCGGAAGACG
GACGCGATTTCTTCGATTTCGTTTCGGGGCGCAAGGAGCCGCCGGCCGGTGTCGGGGCGGGGCTTGTCTATTCACGCTTC
AACCACCCGAACAGCGAGATTGTGGAGGACCGTCTGGCGGTCTATGAGGGGGCGGAAAGTGCAGCCCTTTTCTCGTCCGG
CATGTCCGCCATTGCCACCACGCTTTTCGCTTTTGTGCGCCCGGGCGATGTCATCCTGCATTCGCAGCCACTTTATGGCG
GCACGGAAACGCTTCTGGCGAAGACCTTCTTCAATTTTGGCGTGGAGGCTGTCGCTTTTGCGGATGGTGTTCACGAGGCC
ACCATCGAGAAAGCAGCCGAAGAGGCGCTGGCGAAAGGTCGTGTTTCGGTCATTTTGATCGAAACCCCGGCCAATCCGAC
CAATAGTATTGTCGATGTCGCAGCGGTTCGCCGCGTGGCGGAGAAGATTGAAGCGCGGCAGGGCAGCCGCCCGGTCATCG
CCTGCGACAATACCCTGCTCGGGCCGGTGTTCCAGAAGCCTCTTGATCACGGGGCCGATCTCTCGGTGTATTCGCTGACC
AAATATGTCGGCGGTCATTCCGATCTTATTGCAGGGGCGGTGCTGGGCGCAAAATCCGTTGTGCGCCAGGTAAAGGCCCT
GCGCGGAGCAATCGGTACACAGCTCGATCCGCATTCATGCTGGATGCTTGGCCGCTCCCTGGAAACGCTGGGCCTGCGCA
TGGAGCGGGCTGACAGCAATGCCCACGCCATTGCTGAATTCCTGCGTAATCACCCCAAGGTGGAAAAGCTGCATTATCTG
CCCTTTGCCGATGAGCGTTCCGATATCGCAGCCTTGTTCAAGCGCCAATGTACCGGCGCTGGCTCGACCTTTTCCTTCGA
TATAAAGGGCGGGCAGGCTGCTGCTTTCCGTTTCCTGAATGCGCTGCAAATCCTGAAACTCGCGGTAAGCCTCGGTGGCA
CCGAATCGCTTGCGAGCCATCCCGCGGCGATGACGCATTCAGGTGTTCCGGTCGATGTGCGTGAGCGGATCGGCGTGCTG
GAATCGACCATCCGCCTTTCCATAGGTATCGAGCATCCCGACGACCTGATCGCCGATCTGGCACAGGCTTTGGATGCGGC
CTGA

Upstream 100 bases:

>100_bases
AAGATTCCGTTTCACCTCTGGTAATGTCCTCCTGACCGCCATCCGGCGCGTCCGATCATTCGGACGCTCCGGTCGCGGTA
ATTGTTTTGAGGAGGCAACC

Downstream 100 bases:

>100_bases
TACCAAAATCTAATGCGCTTGACGGTTCCGTTGCAAAAATCCGGTTGTGACGGAACGCGGCTCATCTGCAAATGATGGTC
ATCCGATTGGGTCCATCGCC

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTAPRPSKIRLGNHLLHPETQMLNYGYDSELSEGAVKPPVFLTSTFVFKTAEDGRDFFDFVSGRKEPPAGVGAGLVYSRF
NHPNSEIVEDRLAVYEGAESAALFSSGMSAIATTLFAFVRPGDVILHSQPLYGGTETLLAKTFFNFGVEAVAFADGVHEA
TIEKAAEEALAKGRVSVILIETPANPTNSIVDVAAVRRVAEKIEARQGSRPVIACDNTLLGPVFQKPLDHGADLSVYSLT
KYVGGHSDLIAGAVLGAKSVVRQVKALRGAIGTQLDPHSCWMLGRSLETLGLRMERADSNAHAIAEFLRNHPKVEKLHYL
PFADERSDIAALFKRQCTGAGSTFSFDIKGGQAAAFRFLNALQILKLAVSLGGTESLASHPAAMTHSGVPVDVRERIGVL
ESTIRLSIGIEHPDDLIADLAQALDAA

Sequences:

>Translated_427_residues
MTAPRPSKIRLGNHLLHPETQMLNYGYDSELSEGAVKPPVFLTSTFVFKTAEDGRDFFDFVSGRKEPPAGVGAGLVYSRF
NHPNSEIVEDRLAVYEGAESAALFSSGMSAIATTLFAFVRPGDVILHSQPLYGGTETLLAKTFFNFGVEAVAFADGVHEA
TIEKAAEEALAKGRVSVILIETPANPTNSIVDVAAVRRVAEKIEARQGSRPVIACDNTLLGPVFQKPLDHGADLSVYSLT
KYVGGHSDLIAGAVLGAKSVVRQVKALRGAIGTQLDPHSCWMLGRSLETLGLRMERADSNAHAIAEFLRNHPKVEKLHYL
PFADERSDIAALFKRQCTGAGSTFSFDIKGGQAAAFRFLNALQILKLAVSLGGTESLASHPAAMTHSGVPVDVRERIGVL
ESTIRLSIGIEHPDDLIADLAQALDAA
>Mature_426_residues
TAPRPSKIRLGNHLLHPETQMLNYGYDSELSEGAVKPPVFLTSTFVFKTAEDGRDFFDFVSGRKEPPAGVGAGLVYSRFN
HPNSEIVEDRLAVYEGAESAALFSSGMSAIATTLFAFVRPGDVILHSQPLYGGTETLLAKTFFNFGVEAVAFADGVHEAT
IEKAAEEALAKGRVSVILIETPANPTNSIVDVAAVRRVAEKIEARQGSRPVIACDNTLLGPVFQKPLDHGADLSVYSLTK
YVGGHSDLIAGAVLGAKSVVRQVKALRGAIGTQLDPHSCWMLGRSLETLGLRMERADSNAHAIAEFLRNHPKVEKLHYLP
FADERSDIAALFKRQCTGAGSTFSFDIKGGQAAAFRFLNALQILKLAVSLGGTESLASHPAAMTHSGVPVDVRERIGVLE
STIRLSIGIEHPDDLIADLAQALDAA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=418, Percent_Identity=35.1674641148325, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI299473759, Length=418, Percent_Identity=32.5358851674641, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI25453487, Length=418, Percent_Identity=31.8181818181818, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1790375, Length=405, Percent_Identity=36.5432098765432, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1789383, Length=414, Percent_Identity=28.0193236714976, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71980708, Length=411, Percent_Identity=32.8467153284672, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17538055, Length=396, Percent_Identity=34.5959595959596, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=355, Percent_Identity=37.4647887323944, Blast_Score=197, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=402, Percent_Identity=34.3283582089552, Blast_Score=199, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6321254, Length=425, Percent_Identity=27.2941176470588, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=28.2407407407407, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321904, Length=413, Percent_Identity=29.0556900726392, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=356, Percent_Identity=27.8089887640449, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19922554, Length=399, Percent_Identity=34.5864661654135, Blast_Score=226, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45729; Mature: 45597

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPRPSKIRLGNHLLHPETQMLNYGYDSELSEGAVKPPVFLTSTFVFKTAEDGRDFFDF
CCCCCCCEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHH
VSGRKEPPAGVGAGLVYSRFNHPNSEIVEDRLAVYEGAESAALFSSGMSAIATTLFAFVR
HCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
PGDVILHSQPLYGGTETLLAKTFFNFGVEAVAFADGVHEATIEKAAEEALAKGRVSVILI
CCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEE
ETPANPTNSIVDVAAVRRVAEKIEARQGSRPVIACDNTLLGPVFQKPLDHGADLSVYSLT
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHH
KYVGGHSDLIAGAVLGAKSVVRQVKALRGAIGTQLDPHSCWMLGRSLETLGLRMERADSN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHCCHHHCCCC
AHAIAEFLRNHPKVEKLHYLPFADERSDIAALFKRQCTGAGSTFSFDIKGGQAAAFRFLN
HHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
ALQILKLAVSLGGTESLASHPAAMTHSGVPVDVRERIGVLESTIRLSIGIEHPDDLIADL
HHHHHHHHHHCCCCHHHHCCCCHHCCCCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHH
AQALDAA
HHHHCCC
>Mature Secondary Structure 
TAPRPSKIRLGNHLLHPETQMLNYGYDSELSEGAVKPPVFLTSTFVFKTAEDGRDFFDF
CCCCCCEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHH
VSGRKEPPAGVGAGLVYSRFNHPNSEIVEDRLAVYEGAESAALFSSGMSAIATTLFAFVR
HCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
PGDVILHSQPLYGGTETLLAKTFFNFGVEAVAFADGVHEATIEKAAEEALAKGRVSVILI
CCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEE
ETPANPTNSIVDVAAVRRVAEKIEARQGSRPVIACDNTLLGPVFQKPLDHGADLSVYSLT
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHH
KYVGGHSDLIAGAVLGAKSVVRQVKALRGAIGTQLDPHSCWMLGRSLETLGLRMERADSN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHCCHHHCCCC
AHAIAEFLRNHPKVEKLHYLPFADERSDIAALFKRQCTGAGSTFSFDIKGGQAAAFRFLN
HHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
ALQILKLAVSLGGTESLASHPAAMTHSGVPVDVRERIGVLESTIRLSIGIEHPDDLIADL
HHHHHHHHHHCCCCHHHHCCCCHHCCCCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHH
AQALDAA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]