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The map label for this gene is ansB [H]

Identifier: 225853398

GI number: 225853398

Start: 1909905

End: 1910849

Strand: Reverse

Name: ansB [H]

Synonym: BMEA_A2019

Alternate gene names: 225853398

Gene position: 1910849-1909905 (Counterclockwise)

Preceding gene: 225853400

Following gene: 225853397

Centisome position: 89.89

GC content: 57.35

Gene sequence:

>945_bases
ATGTCTTTAATTTCTATCGGCACCCTCGGGGGTACGATTGCGATGGTTGAGGGGGGAAGCGGAGGCGGTGTTGTGCCGAC
CCTGACCGCGGATGCACTCATTGCCGCTGTGCCGCAGATTAGAAATTATGCGCAGATCAAGGCCCAATCTCTTTTTCAGC
TTCCCAGCCCCTCGCTAAAATTTCGGCACGGCCTTGAAGTGATAGGCTGGGCTGAAAACGAGGTGAAATCGGGAGCGGAC
GGCATAGTGCTGACGCAGGGAACCGATACGCTTGAGGAAATGGCATTCCTTCTGGATTTATTGTGGGAACATCCGCAGCC
CCTCGTACTGACCGGCGCCATGCGCTCGCCGCGCGCGCCGGGTGCTGACGGTCCGGCCAATCTGGTGGCGGCCGTATTGA
CAGTCGCGAGCAGGCTTAGCCGGGAACGCGGTGTCCTTGTTGCAATGAACGATACAATCCACGCGGCACGTTGGATAACA
AAATCTCATGCGCTCAGTGTCCAGACATTCGTATCGTCGGACAGCGGGCCGTTGGGATATGTGTATGAGGAGACGCCGGT
TTACATCAACACCCTTTCGCGGTTGCCAAAAATTGACCGTACGCAGTTCAACGCCGATGTGAAGATCGGCTTTTACGAAT
CGCTGCTGGATGGTGACGCGCAGATGCTGCGAAGCATGTTTGAGAGCGGCAGATATGATGGAATCGTCGTCGCCGGTTTT
GGAGCAGGACATGTGTCGGGCGACGAAGCCGACATTATCGAGCGCTACGCGTCCCGCATTCCGGTCGTCGTAGCCAGCCG
GACCTATGGCGGAAGGACCGCCACGAAGACTTATGGGTTCCATGGCTCCGAGATTGATCTTCAGACGAAAGGCGCTATGC
TCGCAGGCTGGCTATCCCCCCCGCAAGGCGCGGTTATTGCTGTGGGCACTACTGGCAGCGGGTAA

Upstream 100 bases:

>100_bases
TCGGGTTTCAAAAGCGGATTCACATAGACCTCCCCGTGTTGATTCCTATACAAAACTCAAATATGGATAATGCATTATGC
ATAATTTTAGGGAGTTGGAA

Downstream 100 bases:

>100_bases
AAATCTGGAAGAAACAAGAGCTAACTTCCTGCTCTATAACCCATAACTGCCGATCGAGATTCCGCATCCATGGTAAAAGA
GCCACGTTTTAGCAGTCGTC

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSLISIGTLGGTIAMVEGGSGGGVVPTLTADALIAAVPQIRNYAQIKAQSLFQLPSPSLKFRHGLEVIGWAENEVKSGAD
GIVLTQGTDTLEEMAFLLDLLWEHPQPLVLTGAMRSPRAPGADGPANLVAAVLTVASRLSRERGVLVAMNDTIHAARWIT
KSHALSVQTFVSSDSGPLGYVYEETPVYINTLSRLPKIDRTQFNADVKIGFYESLLDGDAQMLRSMFESGRYDGIVVAGF
GAGHVSGDEADIIERYASRIPVVVASRTYGGRTATKTYGFHGSEIDLQTKGAMLAGWLSPPQGAVIAVGTTGSG

Sequences:

>Translated_314_residues
MSLISIGTLGGTIAMVEGGSGGGVVPTLTADALIAAVPQIRNYAQIKAQSLFQLPSPSLKFRHGLEVIGWAENEVKSGAD
GIVLTQGTDTLEEMAFLLDLLWEHPQPLVLTGAMRSPRAPGADGPANLVAAVLTVASRLSRERGVLVAMNDTIHAARWIT
KSHALSVQTFVSSDSGPLGYVYEETPVYINTLSRLPKIDRTQFNADVKIGFYESLLDGDAQMLRSMFESGRYDGIVVAGF
GAGHVSGDEADIIERYASRIPVVVASRTYGGRTATKTYGFHGSEIDLQTKGAMLAGWLSPPQGAVIAVGTTGSG
>Mature_313_residues
SLISIGTLGGTIAMVEGGSGGGVVPTLTADALIAAVPQIRNYAQIKAQSLFQLPSPSLKFRHGLEVIGWAENEVKSGADG
IVLTQGTDTLEEMAFLLDLLWEHPQPLVLTGAMRSPRAPGADGPANLVAAVLTVASRLSRERGVLVAMNDTIHAARWITK
SHALSVQTFVSSDSGPLGYVYEETPVYINTLSRLPKIDRTQFNADVKIGFYESLLDGDAQMLRSMFESGRYDGIVVAGFG
AGHVSGDEADIIERYASRIPVVVASRTYGGRTATKTYGFHGSEIDLQTKGAMLAGWLSPPQGAVIAVGTTGSG

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=281, Percent_Identity=34.1637010676157, Blast_Score=141, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6323189, Length=281, Percent_Identity=32.3843416370107, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323187, Length=281, Percent_Identity=32.3843416370107, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323186, Length=281, Percent_Identity=32.3843416370107, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323184, Length=281, Percent_Identity=32.3843416370107, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320527, Length=315, Percent_Identity=29.2063492063492, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 33147; Mature: 33016

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLISIGTLGGTIAMVEGGSGGGVVPTLTADALIAAVPQIRNYAQIKAQSLFQLPSPSLK
CCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
FRHGLEVIGWAENEVKSGADGIVLTQGTDTLEEMAFLLDLLWEHPQPLVLTGAMRSPRAP
HHCCCEEEEECCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
GADGPANLVAAVLTVASRLSRERGVLVAMNDTIHAARWITKSHALSVQTFVSSDSGPLGY
CCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCEEEEEEECCCCCCEEE
VYEETPVYINTLSRLPKIDRTQFNADVKIGFYESLLDGDAQMLRSMFESGRYDGIVVAGF
EEECCCEEEEHHHHCCCCCCCCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCEEEEEC
GAGHVSGDEADIIERYASRIPVVVASRTYGGRTATKTYGFHGSEIDLQTKGAMLAGWLSP
CCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCCCEEEEEECCCEEEECCCC
PQGAVIAVGTTGSG
CCCCEEEEECCCCC
>Mature Secondary Structure 
SLISIGTLGGTIAMVEGGSGGGVVPTLTADALIAAVPQIRNYAQIKAQSLFQLPSPSLK
CEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
FRHGLEVIGWAENEVKSGADGIVLTQGTDTLEEMAFLLDLLWEHPQPLVLTGAMRSPRAP
HHCCCEEEEECCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
GADGPANLVAAVLTVASRLSRERGVLVAMNDTIHAARWITKSHALSVQTFVSSDSGPLGY
CCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCEEEEEEECCCCCCEEE
VYEETPVYINTLSRLPKIDRTQFNADVKIGFYESLLDGDAQMLRSMFESGRYDGIVVAGF
EEECCCEEEEHHHHCCCCCCCCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCEEEEEC
GAGHVSGDEADIIERYASRIPVVVASRTYGGRTATKTYGFHGSEIDLQTKGAMLAGWLSP
CCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCCCEEEEEECCCEEEECCCC
PQGAVIAVGTTGSG
CCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]