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The map label for this gene is eptA [H]

Identifier: 225853386

GI number: 225853386

Start: 1897529

End: 1899163

Strand: Direct

Name: eptA [H]

Synonym: BMEA_A2005

Alternate gene names: 225853386

Gene position: 1897529-1899163 (Clockwise)

Preceding gene: 225853385

Following gene: 225853387

Centisome position: 89.27

GC content: 55.6

Gene sequence:

>1635_bases
ATGCGGTTTCCACGCCCAACCATTGCCAGCGGCCCGCTCTGTATTCTTACCGCAATCTATCTTCTGGCGTTCACCAATGC
CTCCTTCTGGCAAAAGCTTGGCGCCTATTTCCACGATCATCCGCAAAAACTGATCGTCGCGGGCGCTGCAATCCTGCTCC
TGCACATTGCGCTCCTGCTCGTCTTTTCAGCCAAATATGTCATCAAGCCCGTGCTGATCTTTATGATCCTCGTGGCGGCA
GGCGGCTCCTATTTCACCGCGACATTCGGAACCATCATAACCCGCAGCGTCGTTGAAGCGACCTTTACGACGACGCAGGC
CGAGTCGAACCAGCTTCTGACCACCGGCTTCATCCTGCACATGGTTCTCTACGGCCTCATTCCCTCGCTCCTCATCGTCT
GGGTGCGCGTGAAACATCGCCCGCTTCTGCGCAAGATCGCCGTCAATACCGTGGCAATCACCGCCTGCCTCGTGGGGGCA
GTTGTGCTGATCGGTTCGGATTTCGGAAGCTTTTCCTCCATGTATCGCGAACACCGGTCCGACATCATGGAACGGCTGGA
GCCCATCACCCCCTTGAAAAGCACGATCCAGTACGCAGCAAGCAATCTCAAGGATAACCGCGTCGTGATGCAGCCGCTTG
GCCTTGACGCCAAGCAGACCCTGGCGCCACTGCCTTCCGGCAAGAAACTGCTTACCGTCATCGTTGTTGGTGAAACCGCC
CGCGCAAAAAATTTCAGCCTCTATGGCTACAAGCAGGAAACCAACCCTGAACTTGAAAAACAGGATATCGTCGCCTTCAC
CAATACAGTCAGTTGCGGCACAAGCACGGCTGTTTCAGTGCCCTGCATGTTCTCCCCTTTCACACGCGCGGAATATTCCA
GCACCAAATTTCGCAGTTCGGAAAACCTGATGGACGTGCTGAACCATGCGGGCGTAAAAGTGGCCTGGTATGAAAACAAT
ACCGGCAGCAAGAACGTCGCCGATCGCATTGCTATGGTGGATCTGCAAAACTCCGGTGACACCCGCTATTGCAAGGGCGG
CGAATGCCTTGACCAGATATTGGTCGATTATCTGAAAGAGCACCTGAAGAATTTCAACGGGAATGCAACCATCGTGCTCC
ACATGACGGGCAGCCACGGCCCGGCCTATTACCGCCGCTATCCTGCTGAATTCGCCAGATTCCAGCCGGAATGCCGCACC
AGCGAATTCTCCAATTGCACGAACGAGAAGATCGTTAACGCCTATGACAATACGATCCTCTATACGGATCACATCTTGTC
CGAGGTTATCGATCTCTTGAAATCAAACGAAAGCCGCTTCGCCCCAGCCATGATCTATATGTCCGACCATGGCGAATCGC
TGGGCGAAAACGGGCTTTACCTCCATGCCGCGCCCTATTTCATCGCGCCCAACGAACAGACCCATATTCCCTTCATCGCC
TGGTTCTCGCCGGATTACGCATCCGCGACCGGGCTGGACACGGCATGCCTGAAACAGGGGGCTGCATCTCCCGCCTCGCA
CGACAATCTCTTCCACACCGTCCTTGGCATGATGGGCGTGAAAACCTCGGTCTATGATGCAACCCTCGACCGCTTCGCCT
CCTGCCGCAAGGCGGCGGTGGCATCGCAGAGTTAG

Upstream 100 bases:

>100_bases
TTTGCGTTTACAACTTATGATTGCTAGATGAGATCCTCTTGCTGCAAAATTTCGTTTTATTCATAACCACCAAGATCAAC
GCCAGATTGAAAGCCCAACC

Downstream 100 bases:

>100_bases
TGGGCAGAGCTAGTGGGCAGAGTTAGTGGATCGAATTTGACCTTTGAATCCCGGCTGCCACATATGCTGCTTCAAACGTC
AAATCCTAAAAATCCACTAG

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MRFPRPTIASGPLCILTAIYLLAFTNASFWQKLGAYFHDHPQKLIVAGAAILLLHIALLLVFSAKYVIKPVLIFMILVAA
GGSYFTATFGTIITRSVVEATFTTTQAESNQLLTTGFILHMVLYGLIPSLLIVWVRVKHRPLLRKIAVNTVAITACLVGA
VVLIGSDFGSFSSMYREHRSDIMERLEPITPLKSTIQYAASNLKDNRVVMQPLGLDAKQTLAPLPSGKKLLTVIVVGETA
RAKNFSLYGYKQETNPELEKQDIVAFTNTVSCGTSTAVSVPCMFSPFTRAEYSSTKFRSSENLMDVLNHAGVKVAWYENN
TGSKNVADRIAMVDLQNSGDTRYCKGGECLDQILVDYLKEHLKNFNGNATIVLHMTGSHGPAYYRRYPAEFARFQPECRT
SEFSNCTNEKIVNAYDNTILYTDHILSEVIDLLKSNESRFAPAMIYMSDHGESLGENGLYLHAAPYFIAPNEQTHIPFIA
WFSPDYASATGLDTACLKQGAASPASHDNLFHTVLGMMGVKTSVYDATLDRFASCRKAAVASQS

Sequences:

>Translated_544_residues
MRFPRPTIASGPLCILTAIYLLAFTNASFWQKLGAYFHDHPQKLIVAGAAILLLHIALLLVFSAKYVIKPVLIFMILVAA
GGSYFTATFGTIITRSVVEATFTTTQAESNQLLTTGFILHMVLYGLIPSLLIVWVRVKHRPLLRKIAVNTVAITACLVGA
VVLIGSDFGSFSSMYREHRSDIMERLEPITPLKSTIQYAASNLKDNRVVMQPLGLDAKQTLAPLPSGKKLLTVIVVGETA
RAKNFSLYGYKQETNPELEKQDIVAFTNTVSCGTSTAVSVPCMFSPFTRAEYSSTKFRSSENLMDVLNHAGVKVAWYENN
TGSKNVADRIAMVDLQNSGDTRYCKGGECLDQILVDYLKEHLKNFNGNATIVLHMTGSHGPAYYRRYPAEFARFQPECRT
SEFSNCTNEKIVNAYDNTILYTDHILSEVIDLLKSNESRFAPAMIYMSDHGESLGENGLYLHAAPYFIAPNEQTHIPFIA
WFSPDYASATGLDTACLKQGAASPASHDNLFHTVLGMMGVKTSVYDATLDRFASCRKAAVASQS
>Mature_544_residues
MRFPRPTIASGPLCILTAIYLLAFTNASFWQKLGAYFHDHPQKLIVAGAAILLLHIALLLVFSAKYVIKPVLIFMILVAA
GGSYFTATFGTIITRSVVEATFTTTQAESNQLLTTGFILHMVLYGLIPSLLIVWVRVKHRPLLRKIAVNTVAITACLVGA
VVLIGSDFGSFSSMYREHRSDIMERLEPITPLKSTIQYAASNLKDNRVVMQPLGLDAKQTLAPLPSGKKLLTVIVVGETA
RAKNFSLYGYKQETNPELEKQDIVAFTNTVSCGTSTAVSVPCMFSPFTRAEYSSTKFRSSENLMDVLNHAGVKVAWYENN
TGSKNVADRIAMVDLQNSGDTRYCKGGECLDQILVDYLKEHLKNFNGNATIVLHMTGSHGPAYYRRYPAEFARFQPECRT
SEFSNCTNEKIVNAYDNTILYTDHILSEVIDLLKSNESRFAPAMIYMSDHGESLGENGLYLHAAPYFIAPNEQTHIPFIA
WFSPDYASATGLDTACLKQGAASPASHDNLFHTVLGMMGVKTSVYDATLDRFASCRKAAVASQS

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=487, Percent_Identity=38.3983572895277, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI87082286, Length=563, Percent_Identity=27.1758436944938, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87082223, Length=426, Percent_Identity=25.8215962441315, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1790392, Length=509, Percent_Identity=24.75442043222, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 59933; Mature: 59933

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFPRPTIASGPLCILTAIYLLAFTNASFWQKLGAYFHDHPQKLIVAGAAILLLHIALLL
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VFSAKYVIKPVLIFMILVAAGGSYFTATFGTIITRSVVEATFTTTQAESNQLLTTGFILH
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
MVLYGLIPSLLIVWVRVKHRPLLRKIAVNTVAITACLVGAVVLIGSDFGSFSSMYREHRS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
DIMERLEPITPLKSTIQYAASNLKDNRVVMQPLGLDAKQTLAPLPSGKKLLTVIVVGETA
HHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCCEEEEEEEECCCC
RAKNFSLYGYKQETNPELEKQDIVAFTNTVSCGTSTAVSVPCMFSPFTRAEYSSTKFRSS
CCCCCEEEEECCCCCCCCCHHHHEEEECEECCCCCCEEECCEEECCCCCCCCCCHHHCCH
ENLMDVLNHAGVKVAWYENNTGSKNVADRIAMVDLQNSGDTRYCKGGECLDQILVDYLKE
HHHHHHHHHCCEEEEEEECCCCCCHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
HLKNFNGNATIVLHMTGSHGPAYYRRYPAEFARFQPECRTSEFSNCTNEKIVNAYDNTIL
HHHCCCCCEEEEEEEECCCCCHHHHHCCHHHHHCCCCCCCHHHCCCCCHHHHHHCCCEEE
YTDHILSEVIDLLKSNESRFAPAMIYMSDHGESLGENGLYLHAAPYFIAPNEQTHIPFIA
EHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEECCEEECCCCCCCCCEEE
WFSPDYASATGLDTACLKQGAASPASHDNLFHTVLGMMGVKTSVYDATLDRFASCRKAAV
EECCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ASQS
CCCC
>Mature Secondary Structure
MRFPRPTIASGPLCILTAIYLLAFTNASFWQKLGAYFHDHPQKLIVAGAAILLLHIALLL
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VFSAKYVIKPVLIFMILVAAGGSYFTATFGTIITRSVVEATFTTTQAESNQLLTTGFILH
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
MVLYGLIPSLLIVWVRVKHRPLLRKIAVNTVAITACLVGAVVLIGSDFGSFSSMYREHRS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
DIMERLEPITPLKSTIQYAASNLKDNRVVMQPLGLDAKQTLAPLPSGKKLLTVIVVGETA
HHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCCEEEEEEEECCCC
RAKNFSLYGYKQETNPELEKQDIVAFTNTVSCGTSTAVSVPCMFSPFTRAEYSSTKFRSS
CCCCCEEEEECCCCCCCCCHHHHEEEECEECCCCCCEEECCEEECCCCCCCCCCHHHCCH
ENLMDVLNHAGVKVAWYENNTGSKNVADRIAMVDLQNSGDTRYCKGGECLDQILVDYLKE
HHHHHHHHHCCEEEEEEECCCCCCHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
HLKNFNGNATIVLHMTGSHGPAYYRRYPAEFARFQPECRTSEFSNCTNEKIVNAYDNTIL
HHHCCCCCEEEEEEEECCCCCHHHHHCCHHHHHCCCCCCCHHHCCCCCHHHHHHCCCEEE
YTDHILSEVIDLLKSNESRFAPAMIYMSDHGESLGENGLYLHAAPYFIAPNEQTHIPFIA
EHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEECCEEECCCCCCCCCEEE
WFSPDYASATGLDTACLKQGAASPASHDNLFHTVLGMMGVKTSVYDATLDRFASCRKAAV
EECCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ASQS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]